Immune cell profiling of atopic dermatitis patients before and after treatment with halometasone cream wet-wrap therapy by single-cell sequencing
Objectives: Peripheral blood immune cell profiling of atopic dermatitis patients before and after treatment by single-cell RNA sequencing technique has not been reported. To study the immune Cell Profiling of Atopic Dermatitis Patients Before and After Treatment with Halometasone Cream Wet-Wrap Ther...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Indian Journal of Dermatology |
| Subjects: | |
| Online Access: | http://www.e-ijd.org/article.asp?issn=0019-5154;year=2023;volume=68;issue=1;spage=8;epage=14;aulast=Gu |
| _version_ | 1797824337080745984 |
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| author | Xiao-Guang Gu Xin Yu Bo-Yang Zhou Ming Li Wei Xu Yan Li Lin-Feng Li |
| author_facet | Xiao-Guang Gu Xin Yu Bo-Yang Zhou Ming Li Wei Xu Yan Li Lin-Feng Li |
| author_sort | Xiao-Guang Gu |
| collection | DOAJ |
| description | Objectives: Peripheral blood immune cell profiling of atopic dermatitis patients before and after treatment by single-cell RNA sequencing technique has not been reported. To study the immune Cell Profiling of Atopic Dermatitis Patients Before and After Treatment with Halometasone Cream Wet-Wrap Therapy. Methods: We used single cell sequencing to detect the proportion change and gene expression change of immune cells in 2 patients before and after treatment, and then used real-time PCR to confirm the mRNA level of differential genes. Results: In this study, scRNA-seq in two patients with severe AD before and after halometasone cream wet-wrap therapy showed that in the mild severity of AD after treatment, Th2 cells were significantly decreased (41.2% vs 13.4%), Th1 and Th17 cells were increased (23.3% vs 43.7%, 2.3% vs 4.8% respectively). The proportion of Th22 cells did not change much (1.3% vs 1.9%). Tregs were significantly increased also (1.5% vs 5.0%). In the regulatory T cells, the expression of IL-27, PD-1, CD103, CTLA-4, ZNF-66, IL-β, CD7 gene was specifically increased after treatment, and CD39, P21, TOX2, CD151, CD79A, S100A12, TRAP1 gene was specifically decreased after treatment. In the TH2 cells, the expression of CD27, CD68, EZH1, RAD1, EGFR, CCR10, BCL11A, KLF4 gene was specifically increased after treatment and CCL26, CD180, IL-31, CCL22, LEF1, OX40 gene was specifically decreased after treatment. Conclusions: These genes may be new target for further study. |
| first_indexed | 2024-03-13T10:37:30Z |
| format | Article |
| id | doaj.art-d6044d6ce4f6471db4f806d90900084a |
| institution | Directory Open Access Journal |
| issn | 0019-5154 1998-3611 |
| language | English |
| last_indexed | 2024-03-13T10:37:30Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Dermatology |
| spelling | doaj.art-d6044d6ce4f6471db4f806d90900084a2023-05-18T05:27:54ZengWolters Kluwer Medknow PublicationsIndian Journal of Dermatology0019-51541998-36112023-01-0168181410.4103/ijd.ijd_801_22Immune cell profiling of atopic dermatitis patients before and after treatment with halometasone cream wet-wrap therapy by single-cell sequencingXiao-Guang GuXin YuBo-Yang ZhouMing LiWei XuYan LiLin-Feng LiObjectives: Peripheral blood immune cell profiling of atopic dermatitis patients before and after treatment by single-cell RNA sequencing technique has not been reported. To study the immune Cell Profiling of Atopic Dermatitis Patients Before and After Treatment with Halometasone Cream Wet-Wrap Therapy. Methods: We used single cell sequencing to detect the proportion change and gene expression change of immune cells in 2 patients before and after treatment, and then used real-time PCR to confirm the mRNA level of differential genes. Results: In this study, scRNA-seq in two patients with severe AD before and after halometasone cream wet-wrap therapy showed that in the mild severity of AD after treatment, Th2 cells were significantly decreased (41.2% vs 13.4%), Th1 and Th17 cells were increased (23.3% vs 43.7%, 2.3% vs 4.8% respectively). The proportion of Th22 cells did not change much (1.3% vs 1.9%). Tregs were significantly increased also (1.5% vs 5.0%). In the regulatory T cells, the expression of IL-27, PD-1, CD103, CTLA-4, ZNF-66, IL-β, CD7 gene was specifically increased after treatment, and CD39, P21, TOX2, CD151, CD79A, S100A12, TRAP1 gene was specifically decreased after treatment. In the TH2 cells, the expression of CD27, CD68, EZH1, RAD1, EGFR, CCR10, BCL11A, KLF4 gene was specifically increased after treatment and CCL26, CD180, IL-31, CCL22, LEF1, OX40 gene was specifically decreased after treatment. Conclusions: These genes may be new target for further study.http://www.e-ijd.org/article.asp?issn=0019-5154;year=2023;volume=68;issue=1;spage=8;epage=14;aulast=Gucd39single-cell rna sequencing techniqueth2 cellstox2tregss |
| spellingShingle | Xiao-Guang Gu Xin Yu Bo-Yang Zhou Ming Li Wei Xu Yan Li Lin-Feng Li Immune cell profiling of atopic dermatitis patients before and after treatment with halometasone cream wet-wrap therapy by single-cell sequencing Indian Journal of Dermatology cd39 single-cell rna sequencing technique th2 cells tox2 tregss |
| title | Immune cell profiling of atopic dermatitis patients before and after treatment with halometasone cream wet-wrap therapy by single-cell sequencing |
| title_full | Immune cell profiling of atopic dermatitis patients before and after treatment with halometasone cream wet-wrap therapy by single-cell sequencing |
| title_fullStr | Immune cell profiling of atopic dermatitis patients before and after treatment with halometasone cream wet-wrap therapy by single-cell sequencing |
| title_full_unstemmed | Immune cell profiling of atopic dermatitis patients before and after treatment with halometasone cream wet-wrap therapy by single-cell sequencing |
| title_short | Immune cell profiling of atopic dermatitis patients before and after treatment with halometasone cream wet-wrap therapy by single-cell sequencing |
| title_sort | immune cell profiling of atopic dermatitis patients before and after treatment with halometasone cream wet wrap therapy by single cell sequencing |
| topic | cd39 single-cell rna sequencing technique th2 cells tox2 tregss |
| url | http://www.e-ijd.org/article.asp?issn=0019-5154;year=2023;volume=68;issue=1;spage=8;epage=14;aulast=Gu |
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