Clonal dissemination of the multi-drug resistant <it>Salmonella enterica </it>serovar Braenderup, but not the serovar Bareilly, of prevalent serogroup C1 <it>Salmonella </it>from Taiwan
<p>Abstract</p> <p>Background</p> <p>Nontyphoidal <it>Salmonella </it>is the main cause of human salmonellosis. In order to study the prevalent serogroups and serovars of clinical isolates in Taiwan, 8931 <it>Salmonellae </it>isolates were collec...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-12-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/9/264 |
| _version_ | 1818509158785220608 |
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| author | Tsay Jwu-Guh Weng Bor-Chun Chang Yung-Fu Chen Shu-Wun Chu Chi-Hong Chiu Cheng-Hsun Lin Jui-Ming Chiou Chien-Shun Chen Chyi-Liang Liu Chien-Hsing Chu Chishih |
| author_facet | Tsay Jwu-Guh Weng Bor-Chun Chang Yung-Fu Chen Shu-Wun Chu Chi-Hong Chiu Cheng-Hsun Lin Jui-Ming Chiou Chien-Shun Chen Chyi-Liang Liu Chien-Hsing Chu Chishih |
| author_sort | Tsay Jwu-Guh |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Nontyphoidal <it>Salmonella </it>is the main cause of human salmonellosis. In order to study the prevalent serogroups and serovars of clinical isolates in Taiwan, 8931 <it>Salmonellae </it>isolates were collected from 19 medical centers and district hospitals throughout the country from 2004 to 2007. The pulsed-field eletrophoresis types (PFGE) and antibiotic resistance profiles of <it>Salmonella enterica </it>serovars Bareilly (<it>S</it>. Bareilly) and Braenderup (<it>S</it>. Braenderup) were compared, and multi-drug resistance (MDR) plasmids were characterized.</p> <p>Results</p> <p>Over 95% of human salmonellosis in Taiwan was caused by five <it>Salmonella </it>serogroups: B, C1, C2-C3, D1, and E1. <it>S</it>. Typhymurium, <it>S</it>. Enteritidis, <it>S</it>. Stanley and <it>S</it>. Newport were the four most prevalent serovars, accounting for about 64% of isolates. While only one or two major serovars from four of the most prevalent serogroups were represented, four predominant serovars were found in serogroup C1 <it>Salmonellae</it>. The prevalence was decreasing for <it>S</it>. Choleraeuis and <it>S</it>. Braenderup, and S. Virchow and increasing for <it>S</it>. Bareilly. <it>S</it>. Braenderup mainly caused gastroenteritis in children; in contrast, <it>S</it>. Bareiley infected children and elderly people. Both serovars differed by <it>Xba</it>I-PFGE patterns. Almost all <it>S</it>. Bareilly isolates were susceptible to antibiotics of interest, while all lacked plasmids and belonged to one clone. Two distinct major clones in <it>S</it>. Braenderup were cluster A, mainly including MDR isolates with large MDR plasmid from North Taiwan, and cluster B, mainly containing susceptible isolates without R plasmid from South Taiwan. In cluster A, there were two types of conjugative R plasmids with sizes ranging from 75 to 130 kb. Type 1 plasmids consisted of replicons F1A/F1B, <it>bla</it><sub>TEM</sub>, IS<it>26</it>, and a class 1 integron with the genes <it>dfrA12</it>-<it>orfF</it>-<it>aadA2-qacE</it>Δ1-<it>sulI</it>. Type 2 plasmids belonged to incompatibility group Inc<it>I</it>, contained <it>tnpA</it>-<it>bla</it><sub>CMY-2</sub>-<it>blc</it>-<it>sugE </it>genetic structures and lacked both IS<it>26 </it>and class 1 integrons. Although type 2 plasmids showed higher conjugation capability, type 1 plasmids were the predominant plasmid.</p> <p>Conclusions</p> <p>Serogroups B, C1, C2-C3, D1, and E1 of <it>Salmonella </it>caused over 95% of human salmonellosis. Two prevalent serovars within serogroup C1, <it>S</it>. Bareilly and cluster B of S. Braenderup, were clonal and drug-susceptible. However, cluster A of <it>S</it>. Braenderup was MDR and probably derived from susceptible isolates by acquiring one of two distinct conjugative R plasmids.</p> |
| first_indexed | 2024-12-10T22:41:41Z |
| format | Article |
| id | doaj.art-d604bd7f7bc444259b09ad21c4872853 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-10T22:41:41Z |
| publishDate | 2009-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-d604bd7f7bc444259b09ad21c48728532022-12-22T01:30:42ZengBMCBMC Microbiology1471-21802009-12-019126410.1186/1471-2180-9-264Clonal dissemination of the multi-drug resistant <it>Salmonella enterica </it>serovar Braenderup, but not the serovar Bareilly, of prevalent serogroup C1 <it>Salmonella </it>from TaiwanTsay Jwu-GuhWeng Bor-ChunChang Yung-FuChen Shu-WunChu Chi-HongChiu Cheng-HsunLin Jui-MingChiou Chien-ShunChen Chyi-LiangLiu Chien-HsingChu Chishih<p>Abstract</p> <p>Background</p> <p>Nontyphoidal <it>Salmonella </it>is the main cause of human salmonellosis. In order to study the prevalent serogroups and serovars of clinical isolates in Taiwan, 8931 <it>Salmonellae </it>isolates were collected from 19 medical centers and district hospitals throughout the country from 2004 to 2007. The pulsed-field eletrophoresis types (PFGE) and antibiotic resistance profiles of <it>Salmonella enterica </it>serovars Bareilly (<it>S</it>. Bareilly) and Braenderup (<it>S</it>. Braenderup) were compared, and multi-drug resistance (MDR) plasmids were characterized.</p> <p>Results</p> <p>Over 95% of human salmonellosis in Taiwan was caused by five <it>Salmonella </it>serogroups: B, C1, C2-C3, D1, and E1. <it>S</it>. Typhymurium, <it>S</it>. Enteritidis, <it>S</it>. Stanley and <it>S</it>. Newport were the four most prevalent serovars, accounting for about 64% of isolates. While only one or two major serovars from four of the most prevalent serogroups were represented, four predominant serovars were found in serogroup C1 <it>Salmonellae</it>. The prevalence was decreasing for <it>S</it>. Choleraeuis and <it>S</it>. Braenderup, and S. Virchow and increasing for <it>S</it>. Bareilly. <it>S</it>. Braenderup mainly caused gastroenteritis in children; in contrast, <it>S</it>. Bareiley infected children and elderly people. Both serovars differed by <it>Xba</it>I-PFGE patterns. Almost all <it>S</it>. Bareilly isolates were susceptible to antibiotics of interest, while all lacked plasmids and belonged to one clone. Two distinct major clones in <it>S</it>. Braenderup were cluster A, mainly including MDR isolates with large MDR plasmid from North Taiwan, and cluster B, mainly containing susceptible isolates without R plasmid from South Taiwan. In cluster A, there were two types of conjugative R plasmids with sizes ranging from 75 to 130 kb. Type 1 plasmids consisted of replicons F1A/F1B, <it>bla</it><sub>TEM</sub>, IS<it>26</it>, and a class 1 integron with the genes <it>dfrA12</it>-<it>orfF</it>-<it>aadA2-qacE</it>Δ1-<it>sulI</it>. Type 2 plasmids belonged to incompatibility group Inc<it>I</it>, contained <it>tnpA</it>-<it>bla</it><sub>CMY-2</sub>-<it>blc</it>-<it>sugE </it>genetic structures and lacked both IS<it>26 </it>and class 1 integrons. Although type 2 plasmids showed higher conjugation capability, type 1 plasmids were the predominant plasmid.</p> <p>Conclusions</p> <p>Serogroups B, C1, C2-C3, D1, and E1 of <it>Salmonella </it>caused over 95% of human salmonellosis. Two prevalent serovars within serogroup C1, <it>S</it>. Bareilly and cluster B of S. Braenderup, were clonal and drug-susceptible. However, cluster A of <it>S</it>. Braenderup was MDR and probably derived from susceptible isolates by acquiring one of two distinct conjugative R plasmids.</p>http://www.biomedcentral.com/1471-2180/9/264 |
| spellingShingle | Tsay Jwu-Guh Weng Bor-Chun Chang Yung-Fu Chen Shu-Wun Chu Chi-Hong Chiu Cheng-Hsun Lin Jui-Ming Chiou Chien-Shun Chen Chyi-Liang Liu Chien-Hsing Chu Chishih Clonal dissemination of the multi-drug resistant <it>Salmonella enterica </it>serovar Braenderup, but not the serovar Bareilly, of prevalent serogroup C1 <it>Salmonella </it>from Taiwan BMC Microbiology |
| title | Clonal dissemination of the multi-drug resistant <it>Salmonella enterica </it>serovar Braenderup, but not the serovar Bareilly, of prevalent serogroup C1 <it>Salmonella </it>from Taiwan |
| title_full | Clonal dissemination of the multi-drug resistant <it>Salmonella enterica </it>serovar Braenderup, but not the serovar Bareilly, of prevalent serogroup C1 <it>Salmonella </it>from Taiwan |
| title_fullStr | Clonal dissemination of the multi-drug resistant <it>Salmonella enterica </it>serovar Braenderup, but not the serovar Bareilly, of prevalent serogroup C1 <it>Salmonella </it>from Taiwan |
| title_full_unstemmed | Clonal dissemination of the multi-drug resistant <it>Salmonella enterica </it>serovar Braenderup, but not the serovar Bareilly, of prevalent serogroup C1 <it>Salmonella </it>from Taiwan |
| title_short | Clonal dissemination of the multi-drug resistant <it>Salmonella enterica </it>serovar Braenderup, but not the serovar Bareilly, of prevalent serogroup C1 <it>Salmonella </it>from Taiwan |
| title_sort | clonal dissemination of the multi drug resistant it salmonella enterica it serovar braenderup but not the serovar bareilly of prevalent serogroup c1 it salmonella it from taiwan |
| url | http://www.biomedcentral.com/1471-2180/9/264 |
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