Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor
A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration o...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-01-01
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| Series: | MethodsX |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016120303344 |
| _version_ | 1819056805950521344 |
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| author | Wireeya Chawjiraphan Chayachon Apiwat Khoonsake Segkhoonthod Kiatnida Treerattrakoon Preedee Pinpradup Nuankanya Sathirapongsasuti Prapasiri Pongprayoon Patraporn Luksirikul Patcharee Isarankura-Na-Ayudhya Deanpen Japrung |
| author_facet | Wireeya Chawjiraphan Chayachon Apiwat Khoonsake Segkhoonthod Kiatnida Treerattrakoon Preedee Pinpradup Nuankanya Sathirapongsasuti Prapasiri Pongprayoon Patraporn Luksirikul Patcharee Isarankura-Na-Ayudhya Deanpen Japrung |
| author_sort | Wireeya Chawjiraphan |
| collection | DOAJ |
| description | A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of human serum albumin in urine. The limit of detection of the developed platform is 0.05 µg.mL−1 and the detection range is 0.1–600 µg.mL−1, which covers the albuminuria concentration range present in normal human urine and the urine of the patient with chronic kidney disease. This approach can be modified to measure albuminuria using a high-throughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper than those for the standard automated method. Therefore, this aptasensor has significant potential for commercialization and public use. • Our protocol is customized by using the fluorescence quenching property of graphene oxide and specific binding property of human serum albumin aptamer to detect human serum albumin in urine sample • The limit of detection of our developed platform is 0.05 µg.mL−1 • The detection range of our aptasensor is 0.1–600 µg.mL−1 |
| first_indexed | 2024-12-21T13:29:15Z |
| format | Article |
| id | doaj.art-d604f2bf1f5b4a26baeeec4d18b6858f |
| institution | Directory Open Access Journal |
| issn | 2215-0161 |
| language | English |
| last_indexed | 2024-12-21T13:29:15Z |
| publishDate | 2020-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | MethodsX |
| spelling | doaj.art-d604f2bf1f5b4a26baeeec4d18b6858f2022-12-21T19:02:21ZengElsevierMethodsX2215-01612020-01-017101114Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensorWireeya Chawjiraphan0Chayachon Apiwat1Khoonsake Segkhoonthod2Kiatnida Treerattrakoon3Preedee Pinpradup4Nuankanya Sathirapongsasuti5Prapasiri Pongprayoon6Patraporn Luksirikul7Patcharee Isarankura-Na-Ayudhya8Deanpen Japrung9National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani, ThailandNational Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani, Thailand; Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok, ThailandNational Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani, ThailandNational Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani, Thailand; Department of Pure and Applied Chemistry, Technology and Innovation Centre, University of Strathclyde, Glasgow, United KingdomNational Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani, ThailandSection of Translational Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, ThailandDepartment of Chemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand; Center for Advanced Studies in Nanotechnology for Chemical, Food and Agricultural Industries, KU Institute for Advanced Studies, Kasetsart University, Bangkok, Thailand.Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok, ThailandDepartment of Medical Technology, Faculty of Allied Health Science, Thammasat University, Pathumthani, ThailandNational Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani, Thailand; Corresponding authors.A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of human serum albumin in urine. The limit of detection of the developed platform is 0.05 µg.mL−1 and the detection range is 0.1–600 µg.mL−1, which covers the albuminuria concentration range present in normal human urine and the urine of the patient with chronic kidney disease. This approach can be modified to measure albuminuria using a high-throughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper than those for the standard automated method. Therefore, this aptasensor has significant potential for commercialization and public use. • Our protocol is customized by using the fluorescence quenching property of graphene oxide and specific binding property of human serum albumin aptamer to detect human serum albumin in urine sample • The limit of detection of our developed platform is 0.05 µg.mL−1 • The detection range of our aptasensor is 0.1–600 µg.mL−1http://www.sciencedirect.com/science/article/pii/S2215016120303344AlbuminuriaHuman serum albuminChronic kidney diseaseAptasensorGraphene oxideFluorescence quenching |
| spellingShingle | Wireeya Chawjiraphan Chayachon Apiwat Khoonsake Segkhoonthod Kiatnida Treerattrakoon Preedee Pinpradup Nuankanya Sathirapongsasuti Prapasiri Pongprayoon Patraporn Luksirikul Patcharee Isarankura-Na-Ayudhya Deanpen Japrung Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor MethodsX Albuminuria Human serum albumin Chronic kidney disease Aptasensor Graphene oxide Fluorescence quenching |
| title | Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor |
| title_full | Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor |
| title_fullStr | Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor |
| title_full_unstemmed | Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor |
| title_short | Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor |
| title_sort | albuminuria detection using graphene oxide mediated fluorescence quenching aptasensor |
| topic | Albuminuria Human serum albumin Chronic kidney disease Aptasensor Graphene oxide Fluorescence quenching |
| url | http://www.sciencedirect.com/science/article/pii/S2215016120303344 |
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