Use of temporal immersion systems for the in vitro multiplication of Anthurium andraeanum Lind. var. Lambada shoots

Two methods to improve the efficiency in the multiplication of shoots of Anthurium andraeanum Lind., they were used to optimize the frequencies of immersion of the explants using a System of Temporary Immersion. The relative efficiency of the method conventional means of culture semisolid for the mu...

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Main Authors: Nydia del Rivero Bautista, Elisa Quiala, Daniel Agramante, Raúl Barbón, Wilder Camacho, Lester Morejón, Marta Pérez
Format: Article
Language:English
Published: Universidad Central Marta Abreu de Las Villas 2004-04-01
Series:Biotecnología Vegetal
Online Access:https://revista.ibp.co.cu/index.php/BV/article/view/393
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author Nydia del Rivero Bautista
Elisa Quiala
Daniel Agramante
Raúl Barbón
Wilder Camacho
Lester Morejón
Marta Pérez
author_facet Nydia del Rivero Bautista
Elisa Quiala
Daniel Agramante
Raúl Barbón
Wilder Camacho
Lester Morejón
Marta Pérez
author_sort Nydia del Rivero Bautista
collection DOAJ
description Two methods to improve the efficiency in the multiplication of shoots of Anthurium andraeanum Lind., they were used to optimize the frequencies of immersion of the explants using a System of Temporary Immersion. The relative efficiency of the method conventional means of culture semisolid for the multiplication of shoots was compared with the method of System of Temporary Immersion. The highest rate of multiplication of shoots was reached using six immersions four times a day during two minutes, controlling the immersion cycles achieves plants better developed and bigger multiplication coefficient. The plantlets multiplied through this method were harvested with 2 to 3 cm of longitude containing from two to five leaves after a period of culture of 50 days. The formation of callus, hiperhidricity and other abnormalities were not observed during the process. The plants obtained by this method were taken to rooting in medium of culture semisólid. This system avoids risks of contamination, it increases yields, it reduces manpower costs and of containers. This technique for its relationship benefit-cost has significant implications for the commercial propagation of the Anthurium. Key words: Culture medium, multiplication rate, plantlets.
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spelling doaj.art-d606f74dceb14e25b2f9f51215c84ecf2022-12-21T20:26:22ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472004-04-0142362Use of temporal immersion systems for the in vitro multiplication of Anthurium andraeanum Lind. var. Lambada shootsNydia del Rivero BautistaElisa QuialaDaniel AgramanteRaúl BarbónWilder CamachoLester MorejónMarta PérezTwo methods to improve the efficiency in the multiplication of shoots of Anthurium andraeanum Lind., they were used to optimize the frequencies of immersion of the explants using a System of Temporary Immersion. The relative efficiency of the method conventional means of culture semisolid for the multiplication of shoots was compared with the method of System of Temporary Immersion. The highest rate of multiplication of shoots was reached using six immersions four times a day during two minutes, controlling the immersion cycles achieves plants better developed and bigger multiplication coefficient. The plantlets multiplied through this method were harvested with 2 to 3 cm of longitude containing from two to five leaves after a period of culture of 50 days. The formation of callus, hiperhidricity and other abnormalities were not observed during the process. The plants obtained by this method were taken to rooting in medium of culture semisólid. This system avoids risks of contamination, it increases yields, it reduces manpower costs and of containers. This technique for its relationship benefit-cost has significant implications for the commercial propagation of the Anthurium. Key words: Culture medium, multiplication rate, plantlets.https://revista.ibp.co.cu/index.php/BV/article/view/393
spellingShingle Nydia del Rivero Bautista
Elisa Quiala
Daniel Agramante
Raúl Barbón
Wilder Camacho
Lester Morejón
Marta Pérez
Use of temporal immersion systems for the in vitro multiplication of Anthurium andraeanum Lind. var. Lambada shoots
Biotecnología Vegetal
title Use of temporal immersion systems for the in vitro multiplication of Anthurium andraeanum Lind. var. Lambada shoots
title_full Use of temporal immersion systems for the in vitro multiplication of Anthurium andraeanum Lind. var. Lambada shoots
title_fullStr Use of temporal immersion systems for the in vitro multiplication of Anthurium andraeanum Lind. var. Lambada shoots
title_full_unstemmed Use of temporal immersion systems for the in vitro multiplication of Anthurium andraeanum Lind. var. Lambada shoots
title_short Use of temporal immersion systems for the in vitro multiplication of Anthurium andraeanum Lind. var. Lambada shoots
title_sort use of temporal immersion systems for the in vitro multiplication of anthurium andraeanum lind var lambada shoots
url https://revista.ibp.co.cu/index.php/BV/article/view/393
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