Rapid detection of antimicrobial resistance in methicillin-resistant Staphylococcus aureus using MALDI-TOF mass spectrometry
Antimicrobial resistance is a growing problem in modern healthcare. Most antimicrobial susceptibility tests (AST) require long culture times which delay diagnosis and effective treatment. Our group has previously reported a proof-of-concept demonstration of a rapid AST in Escherichia coli using deut...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1281155/full |
| _version_ | 1797492858079412224 |
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| author | Josiah J. Rensner Paul Lueth Bryan H. Bellaire Orhan Sahin Young Jin Lee |
| author_facet | Josiah J. Rensner Paul Lueth Bryan H. Bellaire Orhan Sahin Young Jin Lee |
| author_sort | Josiah J. Rensner |
| collection | DOAJ |
| description | Antimicrobial resistance is a growing problem in modern healthcare. Most antimicrobial susceptibility tests (AST) require long culture times which delay diagnosis and effective treatment. Our group has previously reported a proof-of-concept demonstration of a rapid AST in Escherichia coli using deuterium labeling and MALDI mass spectrometry. Culturing bacteria in D2O containing media incorporates deuterium in newly synthesized lipids, resulting in a mass shift that can be easily detected by mass spectrometry. The extent of new growth is measured by the average mass of synthesized lipids that can be correlated with resistance in the presence of antimicrobials. In this work, we adapt this procedure to methicillin-resistant Staphylococcus aureus using the Bruker MALDI-TOF Biotyper, a low-cost instrument commonly available in diagnostic laboratories. The susceptible strain showed a significant decrease in average mass in on-target microdroplet cultures after 3 hours of incubation with 10 µg/mL methicillin, while the resistant strain showed consistent labeling regardless of methicillin concentration. This assay allows us to confidently detect methicillin resistance in S. aureus after only 3 hours of culture time and minimal sample processing, reducing the turn-around-time significantly over conventional assays. The success of this work suggests its potential as a rapid AST widely applicable in many clinical microbiology labs with minimal additional costs. |
| first_indexed | 2024-03-10T01:10:44Z |
| format | Article |
| id | doaj.art-d6073394430e4b48b6c423b2162e4ad7 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-03-10T01:10:44Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-d6073394430e4b48b6c423b2162e4ad72023-11-23T14:18:29ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-11-011310.3389/fcimb.2023.12811551281155Rapid detection of antimicrobial resistance in methicillin-resistant Staphylococcus aureus using MALDI-TOF mass spectrometryJosiah J. Rensner0Paul Lueth1Bryan H. Bellaire2Orhan Sahin3Young Jin Lee4Department of Chemistry, Iowa State University, Ames, IA, United StatesDepartment of Veterinary Microbiology and Preventative Medicine, Iowa State University, Ames, IA, United StatesDepartment of Veterinary Microbiology and Preventative Medicine, Iowa State University, Ames, IA, United StatesDepartment of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, IA, United StatesDepartment of Chemistry, Iowa State University, Ames, IA, United StatesAntimicrobial resistance is a growing problem in modern healthcare. Most antimicrobial susceptibility tests (AST) require long culture times which delay diagnosis and effective treatment. Our group has previously reported a proof-of-concept demonstration of a rapid AST in Escherichia coli using deuterium labeling and MALDI mass spectrometry. Culturing bacteria in D2O containing media incorporates deuterium in newly synthesized lipids, resulting in a mass shift that can be easily detected by mass spectrometry. The extent of new growth is measured by the average mass of synthesized lipids that can be correlated with resistance in the presence of antimicrobials. In this work, we adapt this procedure to methicillin-resistant Staphylococcus aureus using the Bruker MALDI-TOF Biotyper, a low-cost instrument commonly available in diagnostic laboratories. The susceptible strain showed a significant decrease in average mass in on-target microdroplet cultures after 3 hours of incubation with 10 µg/mL methicillin, while the resistant strain showed consistent labeling regardless of methicillin concentration. This assay allows us to confidently detect methicillin resistance in S. aureus after only 3 hours of culture time and minimal sample processing, reducing the turn-around-time significantly over conventional assays. The success of this work suggests its potential as a rapid AST widely applicable in many clinical microbiology labs with minimal additional costs.https://www.frontiersin.org/articles/10.3389/fcimb.2023.1281155/fullMRSAdeuteriumantimicrobial resistanceMALDImass spectrometryBiotyper |
| spellingShingle | Josiah J. Rensner Paul Lueth Bryan H. Bellaire Orhan Sahin Young Jin Lee Rapid detection of antimicrobial resistance in methicillin-resistant Staphylococcus aureus using MALDI-TOF mass spectrometry Frontiers in Cellular and Infection Microbiology MRSA deuterium antimicrobial resistance MALDI mass spectrometry Biotyper |
| title | Rapid detection of antimicrobial resistance in methicillin-resistant Staphylococcus aureus using MALDI-TOF mass spectrometry |
| title_full | Rapid detection of antimicrobial resistance in methicillin-resistant Staphylococcus aureus using MALDI-TOF mass spectrometry |
| title_fullStr | Rapid detection of antimicrobial resistance in methicillin-resistant Staphylococcus aureus using MALDI-TOF mass spectrometry |
| title_full_unstemmed | Rapid detection of antimicrobial resistance in methicillin-resistant Staphylococcus aureus using MALDI-TOF mass spectrometry |
| title_short | Rapid detection of antimicrobial resistance in methicillin-resistant Staphylococcus aureus using MALDI-TOF mass spectrometry |
| title_sort | rapid detection of antimicrobial resistance in methicillin resistant staphylococcus aureus using maldi tof mass spectrometry |
| topic | MRSA deuterium antimicrobial resistance MALDI mass spectrometry Biotyper |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1281155/full |
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