Quantitative Proteomics of Urinary Bladder Cancer Cell Lines Identify UAP1 as a Potential Therapeutic Target
Bladder carcinoma (BC) incidence and mortality rates are increasing worldwide. The development of novel therapeutic strategies is required to improve clinical management of this cancer. Aberrant protein expression may lead to cancer initiation and progression. Therefore, the identification of these...
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MDPI AG
2020-07-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/11/7/763 |
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| author | Vinuth N. Puttamallesh Barnali Deb Kirti Gondkar Ankit Jain Bipin Nair Akhilesh Pandey Aditi Chatterjee Harsha Gowda Prashant Kumar |
| author_facet | Vinuth N. Puttamallesh Barnali Deb Kirti Gondkar Ankit Jain Bipin Nair Akhilesh Pandey Aditi Chatterjee Harsha Gowda Prashant Kumar |
| author_sort | Vinuth N. Puttamallesh |
| collection | DOAJ |
| description | Bladder carcinoma (BC) incidence and mortality rates are increasing worldwide. The development of novel therapeutic strategies is required to improve clinical management of this cancer. Aberrant protein expression may lead to cancer initiation and progression. Therefore, the identification of these potential protein targets and limiting their expression levels would provide alternative treatment options. In this study, we utilized a liquid-chromatography tandem mass spectrometry-based global proteomics approach to identify differentially expressed proteins in bladder cancer cell lines. A total of 3913 proteins were identified in this study, of which 479 proteins were overexpressed and 141 proteins were downregulated in 4 out of 6 BC cell lines when compared with normal human urothelial cell line (TERT-NHUC). We evaluated the role of UDP-N-acetylhexosamine pyrophosphorylase (UAP1) in bladder cancer pathogenesis. The silencing of UAP1 led to reduction in proliferation, invasion, colony formation and migration capability of bladder cancer cell lines. Thus, our study reveals UAP1 as a promising therapeutic target for bladder cancer. |
| first_indexed | 2024-03-10T18:37:56Z |
| format | Article |
| id | doaj.art-d60817ba026e4b0b949ca41e6b1276b5 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T18:37:56Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-d60817ba026e4b0b949ca41e6b1276b52023-11-20T06:07:49ZengMDPI AGGenes2073-44252020-07-0111776310.3390/genes11070763Quantitative Proteomics of Urinary Bladder Cancer Cell Lines Identify UAP1 as a Potential Therapeutic TargetVinuth N. Puttamallesh0Barnali Deb1Kirti Gondkar2Ankit Jain3Bipin Nair4Akhilesh Pandey5Aditi Chatterjee6Harsha Gowda7Prashant Kumar8Institute of Bioinformatics, International Technology Park, Bangalore 560066, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore 560066, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore 560066, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore 560066, IndiaAmrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam 690525, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore 560066, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore 560066, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore 560066, IndiaInstitute of Bioinformatics, International Technology Park, Bangalore 560066, IndiaBladder carcinoma (BC) incidence and mortality rates are increasing worldwide. The development of novel therapeutic strategies is required to improve clinical management of this cancer. Aberrant protein expression may lead to cancer initiation and progression. Therefore, the identification of these potential protein targets and limiting their expression levels would provide alternative treatment options. In this study, we utilized a liquid-chromatography tandem mass spectrometry-based global proteomics approach to identify differentially expressed proteins in bladder cancer cell lines. A total of 3913 proteins were identified in this study, of which 479 proteins were overexpressed and 141 proteins were downregulated in 4 out of 6 BC cell lines when compared with normal human urothelial cell line (TERT-NHUC). We evaluated the role of UDP-N-acetylhexosamine pyrophosphorylase (UAP1) in bladder cancer pathogenesis. The silencing of UAP1 led to reduction in proliferation, invasion, colony formation and migration capability of bladder cancer cell lines. Thus, our study reveals UAP1 as a promising therapeutic target for bladder cancer.https://www.mdpi.com/2073-4425/11/7/763quantitative proteomicsurothelial carcinomamolecular subtypestherapeutic target |
| spellingShingle | Vinuth N. Puttamallesh Barnali Deb Kirti Gondkar Ankit Jain Bipin Nair Akhilesh Pandey Aditi Chatterjee Harsha Gowda Prashant Kumar Quantitative Proteomics of Urinary Bladder Cancer Cell Lines Identify UAP1 as a Potential Therapeutic Target Genes quantitative proteomics urothelial carcinoma molecular subtypes therapeutic target |
| title | Quantitative Proteomics of Urinary Bladder Cancer Cell Lines Identify UAP1 as a Potential Therapeutic Target |
| title_full | Quantitative Proteomics of Urinary Bladder Cancer Cell Lines Identify UAP1 as a Potential Therapeutic Target |
| title_fullStr | Quantitative Proteomics of Urinary Bladder Cancer Cell Lines Identify UAP1 as a Potential Therapeutic Target |
| title_full_unstemmed | Quantitative Proteomics of Urinary Bladder Cancer Cell Lines Identify UAP1 as a Potential Therapeutic Target |
| title_short | Quantitative Proteomics of Urinary Bladder Cancer Cell Lines Identify UAP1 as a Potential Therapeutic Target |
| title_sort | quantitative proteomics of urinary bladder cancer cell lines identify uap1 as a potential therapeutic target |
| topic | quantitative proteomics urothelial carcinoma molecular subtypes therapeutic target |
| url | https://www.mdpi.com/2073-4425/11/7/763 |
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