Characterisation of chlorinated, brominated and mixed halogenated dioxins, furans and biphenyls as potent and as partial agonists of the Aryl hydrocarbon receptor
The Aryl hydrocarbon receptor (AhR) binds a variety of chlorinated and brominated dioxins, furans and biphenyls. Mixed halogenated variants have been recently identified in food at significant levels but full characterisation requires potency data in order to gauge their impact on risk assessment. R...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-03-01
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| Series: | Environment International |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0160412014003572 |
| _version_ | 1818615289136283648 |
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| author | Richard J. Wall Alwyn Fernandes Martin Rose David R. Bell Ian R. Mellor |
| author_facet | Richard J. Wall Alwyn Fernandes Martin Rose David R. Bell Ian R. Mellor |
| author_sort | Richard J. Wall |
| collection | DOAJ |
| description | The Aryl hydrocarbon receptor (AhR) binds a variety of chlorinated and brominated dioxins, furans and biphenyls. Mixed halogenated variants have been recently identified in food at significant levels but full characterisation requires potency data in order to gauge their impact on risk assessment. Rat H4IIE and human MCF-7 cells were treated with various mixed halogenated ligands. Antagonist properties were measured by treating cells with various concentrations of TCDD in the presence of EC25 of the putative antagonist. Measurement of CYP1A1 RNA was used to quantify the potency of agonism and antagonism. The PXDDs were found to be slightly less potent than the corresponding fully chlorinated congeners with the exception of 2-B,3,7,8-TriCDD which was 2-fold more potent than TCDD. PXDFs and non-ortho-PXBs were found to be more potent than their chlorinated congeners whilst several mono-ortho-substituted PXBs were shown to have partial agonistic properties. REPs were produced for a range of mixed halogenated AhR-activating ligands providing a more accurate estimation of potency for risk assessment. Several environmentally abundant biphenyls were shown to be antagonists and reduce the ability of TCDD to induce CYP1A1. The demonstration of antagonism for AhR ligands represents a challenge for existing REP risk assessment schemes for AhR ligands. Keywords: AhR, Aryl hydrocarbon receptor, Dioxin, Furan, PCB, TCDD, Risk assessment, CYP1A1, Potency |
| first_indexed | 2024-12-16T16:31:32Z |
| format | Article |
| id | doaj.art-d608db2c341b47a1ba80bc327446d548 |
| institution | Directory Open Access Journal |
| issn | 0160-4120 |
| language | English |
| last_indexed | 2024-12-16T16:31:32Z |
| publishDate | 2015-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Environment International |
| spelling | doaj.art-d608db2c341b47a1ba80bc327446d5482022-12-21T22:24:35ZengElsevierEnvironment International0160-41202015-03-01764956Characterisation of chlorinated, brominated and mixed halogenated dioxins, furans and biphenyls as potent and as partial agonists of the Aryl hydrocarbon receptorRichard J. Wall0Alwyn Fernandes1Martin Rose2David R. Bell3Ian R. Mellor4School of Life Sciences, University Park, University of Nottingham, Nottingham, NG7 2RD, UKThe Food and Environment Research Agency, Sand Hutton, York, YO41 1LZ, UKThe Food and Environment Research Agency, Sand Hutton, York, YO41 1LZ, UKEuropean Chemicals Agency, Annankatu 18, P.O. Box 400, FI-00121 Helsinki, FinlandSchool of Life Sciences, University Park, University of Nottingham, Nottingham, NG7 2RD, UK; Corresponding author at: School of Life Sciences, University Park, University of Nottingham, NG7 2RD, UK.The Aryl hydrocarbon receptor (AhR) binds a variety of chlorinated and brominated dioxins, furans and biphenyls. Mixed halogenated variants have been recently identified in food at significant levels but full characterisation requires potency data in order to gauge their impact on risk assessment. Rat H4IIE and human MCF-7 cells were treated with various mixed halogenated ligands. Antagonist properties were measured by treating cells with various concentrations of TCDD in the presence of EC25 of the putative antagonist. Measurement of CYP1A1 RNA was used to quantify the potency of agonism and antagonism. The PXDDs were found to be slightly less potent than the corresponding fully chlorinated congeners with the exception of 2-B,3,7,8-TriCDD which was 2-fold more potent than TCDD. PXDFs and non-ortho-PXBs were found to be more potent than their chlorinated congeners whilst several mono-ortho-substituted PXBs were shown to have partial agonistic properties. REPs were produced for a range of mixed halogenated AhR-activating ligands providing a more accurate estimation of potency for risk assessment. Several environmentally abundant biphenyls were shown to be antagonists and reduce the ability of TCDD to induce CYP1A1. The demonstration of antagonism for AhR ligands represents a challenge for existing REP risk assessment schemes for AhR ligands. Keywords: AhR, Aryl hydrocarbon receptor, Dioxin, Furan, PCB, TCDD, Risk assessment, CYP1A1, Potencyhttp://www.sciencedirect.com/science/article/pii/S0160412014003572 |
| spellingShingle | Richard J. Wall Alwyn Fernandes Martin Rose David R. Bell Ian R. Mellor Characterisation of chlorinated, brominated and mixed halogenated dioxins, furans and biphenyls as potent and as partial agonists of the Aryl hydrocarbon receptor Environment International |
| title | Characterisation of chlorinated, brominated and mixed halogenated dioxins, furans and biphenyls as potent and as partial agonists of the Aryl hydrocarbon receptor |
| title_full | Characterisation of chlorinated, brominated and mixed halogenated dioxins, furans and biphenyls as potent and as partial agonists of the Aryl hydrocarbon receptor |
| title_fullStr | Characterisation of chlorinated, brominated and mixed halogenated dioxins, furans and biphenyls as potent and as partial agonists of the Aryl hydrocarbon receptor |
| title_full_unstemmed | Characterisation of chlorinated, brominated and mixed halogenated dioxins, furans and biphenyls as potent and as partial agonists of the Aryl hydrocarbon receptor |
| title_short | Characterisation of chlorinated, brominated and mixed halogenated dioxins, furans and biphenyls as potent and as partial agonists of the Aryl hydrocarbon receptor |
| title_sort | characterisation of chlorinated brominated and mixed halogenated dioxins furans and biphenyls as potent and as partial agonists of the aryl hydrocarbon receptor |
| url | http://www.sciencedirect.com/science/article/pii/S0160412014003572 |
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