Cannabinerol (CBNR) Influences Synaptic Genes Associated with Cytoskeleton and Ion Channels in NSC-34 Cell Line: A Transcriptomic Study
Cannabinoids are receiving great attention as a novel approach in the treatment of cognitive and motor disabilities, which characterize neurological disorders. To date, over 100 phytocannabinoids have been extracted from <i>Cannabis sativa</i>, and some of them have shown neuroprotective...
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MDPI AG
2024-01-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/12/1/189 |
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| author | Osvaldo Artimagnella Emanuela Mazzon Stefano Salamone Federica Pollastro Agnese Gugliandolo Luigi Chiricosta |
| author_facet | Osvaldo Artimagnella Emanuela Mazzon Stefano Salamone Federica Pollastro Agnese Gugliandolo Luigi Chiricosta |
| author_sort | Osvaldo Artimagnella |
| collection | DOAJ |
| description | Cannabinoids are receiving great attention as a novel approach in the treatment of cognitive and motor disabilities, which characterize neurological disorders. To date, over 100 phytocannabinoids have been extracted from <i>Cannabis sativa</i>, and some of them have shown neuroprotective properties and the capacity to influence synaptic transmission. In this study, we investigated the effects of a less-known phytocannabinoid, cannabinerol (CBNR), on neuronal physiology. Using the NSC-34 motor-neuron-like cell line and next-generation sequencing analysis, we discovered that CBNR influences synaptic genes associated with synapse organization and specialization, including genes related to the cytoskeleton and ion channels. Specifically, the calcium, sodium, and potassium channel subunits (<i>Cacna1b</i>, <i>Cacna1c</i>, <i>Cacnb1</i>, <i>Grin1</i>, <i>Scn8a</i>, <i>Kcnc1</i>, <i>Kcnj9</i>) were upregulated, along with genes related to NMDAR (<i>Agap3</i>, <i>Syngap1</i>) and calcium (<i>Cabp1</i>, <i>Camkv</i>) signaling. Moreover, cytoskeletal and cytoskeleton-associated genes (<i>Actn2</i>, <i>Ina</i>, <i>Trio</i>, <i>Marcks</i>, <i>Bsn</i>, <i>Rtn4</i>, <i>Dgkz</i>, <i>Htt</i>) were also regulated by CBNR. These findings highlight the important role played by CBNR in the regulation of synaptogenesis and synaptic transmission, suggesting the need for further studies to evaluate the neuroprotective role of CBNR in the treatment of synaptic dysfunctions that characterize motor disabilities in many neurological disorders. |
| first_indexed | 2024-03-08T09:57:16Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-08T09:57:16Z |
| publishDate | 2024-01-01 |
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| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-d60b5fdf24294de3a71f6f508afe88902024-01-29T13:47:47ZengMDPI AGBiomedicines2227-90592024-01-0112118910.3390/biomedicines12010189Cannabinerol (CBNR) Influences Synaptic Genes Associated with Cytoskeleton and Ion Channels in NSC-34 Cell Line: A Transcriptomic StudyOsvaldo Artimagnella0Emanuela Mazzon1Stefano Salamone2Federica Pollastro3Agnese Gugliandolo4Luigi Chiricosta5IRCCS Centro Neurolesi “Bonino-Pulejo”, Via Provinciale Palermo, Contrada Casazza, 98124 Messina, ItalyIRCCS Centro Neurolesi “Bonino-Pulejo”, Via Provinciale Palermo, Contrada Casazza, 98124 Messina, ItalyDepartment of Pharmaceutical Sciences, University of Eastern Piedmont, Largo Donegani 2, 28100 Novara, ItalyDepartment of Pharmaceutical Sciences, University of Eastern Piedmont, Largo Donegani 2, 28100 Novara, ItalyIRCCS Centro Neurolesi “Bonino-Pulejo”, Via Provinciale Palermo, Contrada Casazza, 98124 Messina, ItalyIRCCS Centro Neurolesi “Bonino-Pulejo”, Via Provinciale Palermo, Contrada Casazza, 98124 Messina, ItalyCannabinoids are receiving great attention as a novel approach in the treatment of cognitive and motor disabilities, which characterize neurological disorders. To date, over 100 phytocannabinoids have been extracted from <i>Cannabis sativa</i>, and some of them have shown neuroprotective properties and the capacity to influence synaptic transmission. In this study, we investigated the effects of a less-known phytocannabinoid, cannabinerol (CBNR), on neuronal physiology. Using the NSC-34 motor-neuron-like cell line and next-generation sequencing analysis, we discovered that CBNR influences synaptic genes associated with synapse organization and specialization, including genes related to the cytoskeleton and ion channels. Specifically, the calcium, sodium, and potassium channel subunits (<i>Cacna1b</i>, <i>Cacna1c</i>, <i>Cacnb1</i>, <i>Grin1</i>, <i>Scn8a</i>, <i>Kcnc1</i>, <i>Kcnj9</i>) were upregulated, along with genes related to NMDAR (<i>Agap3</i>, <i>Syngap1</i>) and calcium (<i>Cabp1</i>, <i>Camkv</i>) signaling. Moreover, cytoskeletal and cytoskeleton-associated genes (<i>Actn2</i>, <i>Ina</i>, <i>Trio</i>, <i>Marcks</i>, <i>Bsn</i>, <i>Rtn4</i>, <i>Dgkz</i>, <i>Htt</i>) were also regulated by CBNR. These findings highlight the important role played by CBNR in the regulation of synaptogenesis and synaptic transmission, suggesting the need for further studies to evaluate the neuroprotective role of CBNR in the treatment of synaptic dysfunctions that characterize motor disabilities in many neurological disorders.https://www.mdpi.com/2227-9059/12/1/189cannabinerolsynapsephytocannabinoidscytoskeletonion channelstranscriptomic analysis |
| spellingShingle | Osvaldo Artimagnella Emanuela Mazzon Stefano Salamone Federica Pollastro Agnese Gugliandolo Luigi Chiricosta Cannabinerol (CBNR) Influences Synaptic Genes Associated with Cytoskeleton and Ion Channels in NSC-34 Cell Line: A Transcriptomic Study Biomedicines cannabinerol synapse phytocannabinoids cytoskeleton ion channels transcriptomic analysis |
| title | Cannabinerol (CBNR) Influences Synaptic Genes Associated with Cytoskeleton and Ion Channels in NSC-34 Cell Line: A Transcriptomic Study |
| title_full | Cannabinerol (CBNR) Influences Synaptic Genes Associated with Cytoskeleton and Ion Channels in NSC-34 Cell Line: A Transcriptomic Study |
| title_fullStr | Cannabinerol (CBNR) Influences Synaptic Genes Associated with Cytoskeleton and Ion Channels in NSC-34 Cell Line: A Transcriptomic Study |
| title_full_unstemmed | Cannabinerol (CBNR) Influences Synaptic Genes Associated with Cytoskeleton and Ion Channels in NSC-34 Cell Line: A Transcriptomic Study |
| title_short | Cannabinerol (CBNR) Influences Synaptic Genes Associated with Cytoskeleton and Ion Channels in NSC-34 Cell Line: A Transcriptomic Study |
| title_sort | cannabinerol cbnr influences synaptic genes associated with cytoskeleton and ion channels in nsc 34 cell line a transcriptomic study |
| topic | cannabinerol synapse phytocannabinoids cytoskeleton ion channels transcriptomic analysis |
| url | https://www.mdpi.com/2227-9059/12/1/189 |
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