Novel Blotting Method for Mass Spectrometry Imaging of Metabolites in Strawberry Fruit by Desorption/Ionization Using Through Hole Alumina Membrane
Mass spectrometry imaging (MSI) using matrix-assisted laser desorption/ionization (MALDI) is a powerful technique for visualizing metabolites in the strawberry fruit. During sample preparation for MALDI-MSI, sectioning of the samples is usually required. In general, MALDI-MSI analysis of strawberry...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/9/4/408 |
| _version_ | 1797571773478207488 |
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| author | Hirofumi Enomoto Masahiro Kotani Takayuki Ohmura |
| author_facet | Hirofumi Enomoto Masahiro Kotani Takayuki Ohmura |
| author_sort | Hirofumi Enomoto |
| collection | DOAJ |
| description | Mass spectrometry imaging (MSI) using matrix-assisted laser desorption/ionization (MALDI) is a powerful technique for visualizing metabolites in the strawberry fruit. During sample preparation for MALDI-MSI, sectioning of the samples is usually required. In general, MALDI-MSI analysis of strawberry fruits that are larger than a single glass slide is difficult because thin sections cannot be prepared. In this study, we attempted to visualize metabolites in large strawberry fruits by MSI, employing a blotting method that uses desorption ionization using a through-hole alumina membrane (DIUTHAME) chip. Large strawberry fruits were cut and a DIUTHAME chip was set on the cross-section to blot the metabolites. After drying the DIUTHAME chip, the metabolites were measured in positive and negative ion modes using a commercial MALDI-type mass spectrometer. Several peaks were detected in both the ion modes. Various metabolites related to food quality, such as sugars, organic acids, and anthocyanins, were detected and successfully visualized by blotting on a DIUTHAME chip in MSI. These results suggest that blotting using a DIUTHAME chip in MSI is useful for visualizing the metabolites present in the strawberry fruit. |
| first_indexed | 2024-03-10T20:45:21Z |
| format | Article |
| id | doaj.art-d60becef5ae443aca7a52be2d5f19c1f |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T20:45:21Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-d60becef5ae443aca7a52be2d5f19c1f2023-11-19T20:23:11ZengMDPI AGFoods2304-81582020-04-019440810.3390/foods9040408Novel Blotting Method for Mass Spectrometry Imaging of Metabolites in Strawberry Fruit by Desorption/Ionization Using Through Hole Alumina MembraneHirofumi Enomoto0Masahiro Kotani1Takayuki Ohmura2Department of Biosciences, Faculty of Science and Engineering, Teikyo University, Utsunomiya 320-8551, JapanHamamatsu Photonics K.K., 314-5 Shimokanzo, Iwata 438-0193, JapanHamamatsu Photonics K.K., 314-5 Shimokanzo, Iwata 438-0193, JapanMass spectrometry imaging (MSI) using matrix-assisted laser desorption/ionization (MALDI) is a powerful technique for visualizing metabolites in the strawberry fruit. During sample preparation for MALDI-MSI, sectioning of the samples is usually required. In general, MALDI-MSI analysis of strawberry fruits that are larger than a single glass slide is difficult because thin sections cannot be prepared. In this study, we attempted to visualize metabolites in large strawberry fruits by MSI, employing a blotting method that uses desorption ionization using a through-hole alumina membrane (DIUTHAME) chip. Large strawberry fruits were cut and a DIUTHAME chip was set on the cross-section to blot the metabolites. After drying the DIUTHAME chip, the metabolites were measured in positive and negative ion modes using a commercial MALDI-type mass spectrometer. Several peaks were detected in both the ion modes. Various metabolites related to food quality, such as sugars, organic acids, and anthocyanins, were detected and successfully visualized by blotting on a DIUTHAME chip in MSI. These results suggest that blotting using a DIUTHAME chip in MSI is useful for visualizing the metabolites present in the strawberry fruit.https://www.mdpi.com/2304-8158/9/4/408strawberrymetabolitesmass spectrometry imagingdesorption ionization using through hole alumina membrane (DIUTHAME)blotting |
| spellingShingle | Hirofumi Enomoto Masahiro Kotani Takayuki Ohmura Novel Blotting Method for Mass Spectrometry Imaging of Metabolites in Strawberry Fruit by Desorption/Ionization Using Through Hole Alumina Membrane Foods strawberry metabolites mass spectrometry imaging desorption ionization using through hole alumina membrane (DIUTHAME) blotting |
| title | Novel Blotting Method for Mass Spectrometry Imaging of Metabolites in Strawberry Fruit by Desorption/Ionization Using Through Hole Alumina Membrane |
| title_full | Novel Blotting Method for Mass Spectrometry Imaging of Metabolites in Strawberry Fruit by Desorption/Ionization Using Through Hole Alumina Membrane |
| title_fullStr | Novel Blotting Method for Mass Spectrometry Imaging of Metabolites in Strawberry Fruit by Desorption/Ionization Using Through Hole Alumina Membrane |
| title_full_unstemmed | Novel Blotting Method for Mass Spectrometry Imaging of Metabolites in Strawberry Fruit by Desorption/Ionization Using Through Hole Alumina Membrane |
| title_short | Novel Blotting Method for Mass Spectrometry Imaging of Metabolites in Strawberry Fruit by Desorption/Ionization Using Through Hole Alumina Membrane |
| title_sort | novel blotting method for mass spectrometry imaging of metabolites in strawberry fruit by desorption ionization using through hole alumina membrane |
| topic | strawberry metabolites mass spectrometry imaging desorption ionization using through hole alumina membrane (DIUTHAME) blotting |
| url | https://www.mdpi.com/2304-8158/9/4/408 |
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