Inflammasomes in placental explants of women with preeclampsia cultured with monosodium urate may be modulated by vitamin D
Objectives Preeclampsia (PE) is an important syndrome of gestation characterized by placental and systemic inflammation. High plasma concentration of uric acid are frequently associated with inflammation and endothelial dysfunction and may contribute to PE pathogenesis. This study aimed to evaluate...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-04-01
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| Series: | Hypertension in Pregnancy |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/10641955.2022.2063330 |
| _version_ | 1797681548940541952 |
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| author | Priscila Rezeck Nunes Mariana Romao-Veiga Vanessa Rocha Ribeiro Larissa Ragozo Cardoso de Oliveira Igor de Carvalho Depra Leandro Gustavo de Oliveira Jose Carlos Peracoli Maria Terezinha Serrao Peracoli |
| author_facet | Priscila Rezeck Nunes Mariana Romao-Veiga Vanessa Rocha Ribeiro Larissa Ragozo Cardoso de Oliveira Igor de Carvalho Depra Leandro Gustavo de Oliveira Jose Carlos Peracoli Maria Terezinha Serrao Peracoli |
| author_sort | Priscila Rezeck Nunes |
| collection | DOAJ |
| description | Objectives Preeclampsia (PE) is an important syndrome of gestation characterized by placental and systemic inflammation. High plasma concentration of uric acid are frequently associated with inflammation and endothelial dysfunction and may contribute to PE pathogenesis. This study aimed to evaluate the vitamin D (VD) immunomodulatory effect on the NLRP1/NLRP3 inflammasomes in placental explants from preeclamptic (PE) and normotensive (NT) pregnant women. Study design Placental explants from 10 late-onset PE (LOPE), 10 early-onset PE (EOPE), and 10 NT pregnant women were cultured with or without monosodium urate (MSU) and VD. Main outcome measures Gene and protein expression of NLRP1, NLRP3, HMGB1, caspase-1, interleukin-1 beta (IL-1β), and IL-18 were determined by quantitative PCR and Western blotting/ELISA. Statistical significance was accepted at p < 0.05. Results Basal gene and protein expression of NLRP1/NLRP3 and IL-1β, IL-18 and HMGB1 were significantly higher in explants from EOPE compared to LOPE and NT pregnant women. In addition, culture with MSU increased these inflammatory markers, and concomitant treatment with MSU+VD decreased this effect. Conclusions The results demonstrated that NLRP1 and NLRP3 inflammasomes are upregulated in the placental tissue of EOPE women, associated with high production of inflammatory cytokines. The in vitro treatment with VD downregulated placental inflammasomes induced by MSU, suggesting its immunomodulatory role in the systemic inflammation of PE. |
| first_indexed | 2024-03-11T23:46:27Z |
| format | Article |
| id | doaj.art-d60d168ea4dd4d8fba64975bb050d8a2 |
| institution | Directory Open Access Journal |
| issn | 1064-1955 1525-6065 |
| language | English |
| last_indexed | 2024-03-11T23:46:27Z |
| publishDate | 2022-04-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Hypertension in Pregnancy |
| spelling | doaj.art-d60d168ea4dd4d8fba64975bb050d8a22023-09-19T09:24:44ZengTaylor & Francis GroupHypertension in Pregnancy1064-19551525-60652022-04-0141213914810.1080/10641955.2022.20633302063330Inflammasomes in placental explants of women with preeclampsia cultured with monosodium urate may be modulated by vitamin DPriscila Rezeck Nunes0Mariana Romao-Veiga1Vanessa Rocha Ribeiro2Larissa Ragozo Cardoso de Oliveira3Igor de Carvalho Depra4Leandro Gustavo de Oliveira5Jose Carlos Peracoli6Maria Terezinha Serrao Peracoli7Sao Paulo State University (Unesp)Sao Paulo State University (Unesp)Sao Paulo State University (Unesp)Sao Paulo State University (unesp)Sao Paulo State University (Unesp)Sao Paulo State University (Unesp)Sao Paulo State University (Unesp)Sao Paulo State University (unesp)Objectives Preeclampsia (PE) is an important syndrome of gestation characterized by placental and systemic inflammation. High plasma concentration of uric acid are frequently associated with inflammation and endothelial dysfunction and may contribute to PE pathogenesis. This study aimed to evaluate the vitamin D (VD) immunomodulatory effect on the NLRP1/NLRP3 inflammasomes in placental explants from preeclamptic (PE) and normotensive (NT) pregnant women. Study design Placental explants from 10 late-onset PE (LOPE), 10 early-onset PE (EOPE), and 10 NT pregnant women were cultured with or without monosodium urate (MSU) and VD. Main outcome measures Gene and protein expression of NLRP1, NLRP3, HMGB1, caspase-1, interleukin-1 beta (IL-1β), and IL-18 were determined by quantitative PCR and Western blotting/ELISA. Statistical significance was accepted at p < 0.05. Results Basal gene and protein expression of NLRP1/NLRP3 and IL-1β, IL-18 and HMGB1 were significantly higher in explants from EOPE compared to LOPE and NT pregnant women. In addition, culture with MSU increased these inflammatory markers, and concomitant treatment with MSU+VD decreased this effect. Conclusions The results demonstrated that NLRP1 and NLRP3 inflammasomes are upregulated in the placental tissue of EOPE women, associated with high production of inflammatory cytokines. The in vitro treatment with VD downregulated placental inflammasomes induced by MSU, suggesting its immunomodulatory role in the systemic inflammation of PE.http://dx.doi.org/10.1080/10641955.2022.2063330preeclampsiaplacental explantsmsuinflammasomecytokinesvitamin d |
| spellingShingle | Priscila Rezeck Nunes Mariana Romao-Veiga Vanessa Rocha Ribeiro Larissa Ragozo Cardoso de Oliveira Igor de Carvalho Depra Leandro Gustavo de Oliveira Jose Carlos Peracoli Maria Terezinha Serrao Peracoli Inflammasomes in placental explants of women with preeclampsia cultured with monosodium urate may be modulated by vitamin D Hypertension in Pregnancy preeclampsia placental explants msu inflammasome cytokines vitamin d |
| title | Inflammasomes in placental explants of women with preeclampsia cultured with monosodium urate may be modulated by vitamin D |
| title_full | Inflammasomes in placental explants of women with preeclampsia cultured with monosodium urate may be modulated by vitamin D |
| title_fullStr | Inflammasomes in placental explants of women with preeclampsia cultured with monosodium urate may be modulated by vitamin D |
| title_full_unstemmed | Inflammasomes in placental explants of women with preeclampsia cultured with monosodium urate may be modulated by vitamin D |
| title_short | Inflammasomes in placental explants of women with preeclampsia cultured with monosodium urate may be modulated by vitamin D |
| title_sort | inflammasomes in placental explants of women with preeclampsia cultured with monosodium urate may be modulated by vitamin d |
| topic | preeclampsia placental explants msu inflammasome cytokines vitamin d |
| url | http://dx.doi.org/10.1080/10641955.2022.2063330 |
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