Application of cofactors in the regulation of microbial metabolism: A state of the art review
Cofactors are crucial chemicals that maintain cellular redox balance and drive the cell to do synthetic and catabolic reactions. They are involved in practically all enzymatic activities that occur in live cells. It has been a hot research topic in recent years to manage their concentrations and for...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1145784/full |
| _version_ | 1797849268774502400 |
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| author | Yang Sun Ting Zhang Bingqian Lu Xiangfei Li Ling Jiang |
| author_facet | Yang Sun Ting Zhang Bingqian Lu Xiangfei Li Ling Jiang |
| author_sort | Yang Sun |
| collection | DOAJ |
| description | Cofactors are crucial chemicals that maintain cellular redox balance and drive the cell to do synthetic and catabolic reactions. They are involved in practically all enzymatic activities that occur in live cells. It has been a hot research topic in recent years to manage their concentrations and forms in microbial cells by using appropriate techniques to obtain more high-quality target products. In this review, we first summarize the physiological functions of common cofactors, and give a brief overview of common cofactors acetyl coenzyme A, NAD(P)H/NAD(P)+, and ATP/ADP; then we provide a detailed introduction of intracellular cofactor regeneration pathways, review the regulation of cofactor forms and concentrations by molecular biological means, and review the existing regulatory strategies of microbial cellular cofactors and their application progress, to maximize and rapidly direct the metabolic flux to target metabolites. Finally, we speculate on the future of cofactor engineering applications in cell factories.
Graphical Abstract |
| first_indexed | 2024-04-09T18:42:17Z |
| format | Article |
| id | doaj.art-d60d282bf85940a3bf930b7a2a59c5d0 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-09T18:42:17Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-d60d282bf85940a3bf930b7a2a59c5d02023-04-11T04:32:35ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-04-011410.3389/fmicb.2023.11457841145784Application of cofactors in the regulation of microbial metabolism: A state of the art reviewYang Sun0Ting Zhang1Bingqian Lu2Xiangfei Li3Ling Jiang4State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, Nanjing, ChinaState Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, Nanjing, ChinaState Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, Nanjing, ChinaEngineering Laboratory for Industrial Microbiology Molecular Beeding of Anhui Province, College of Biologic and Food Engineering, Anhui Polytechnic University, Wuhu, ChinaState Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, Nanjing, ChinaCofactors are crucial chemicals that maintain cellular redox balance and drive the cell to do synthetic and catabolic reactions. They are involved in practically all enzymatic activities that occur in live cells. It has been a hot research topic in recent years to manage their concentrations and forms in microbial cells by using appropriate techniques to obtain more high-quality target products. In this review, we first summarize the physiological functions of common cofactors, and give a brief overview of common cofactors acetyl coenzyme A, NAD(P)H/NAD(P)+, and ATP/ADP; then we provide a detailed introduction of intracellular cofactor regeneration pathways, review the regulation of cofactor forms and concentrations by molecular biological means, and review the existing regulatory strategies of microbial cellular cofactors and their application progress, to maximize and rapidly direct the metabolic flux to target metabolites. Finally, we speculate on the future of cofactor engineering applications in cell factories. Graphical Abstracthttps://www.frontiersin.org/articles/10.3389/fmicb.2023.1145784/fullcofactorsmetabolic engineeringregulatory strategiesmicrobial cell factorybiological manufacturing |
| spellingShingle | Yang Sun Ting Zhang Bingqian Lu Xiangfei Li Ling Jiang Application of cofactors in the regulation of microbial metabolism: A state of the art review Frontiers in Microbiology cofactors metabolic engineering regulatory strategies microbial cell factory biological manufacturing |
| title | Application of cofactors in the regulation of microbial metabolism: A state of the art review |
| title_full | Application of cofactors in the regulation of microbial metabolism: A state of the art review |
| title_fullStr | Application of cofactors in the regulation of microbial metabolism: A state of the art review |
| title_full_unstemmed | Application of cofactors in the regulation of microbial metabolism: A state of the art review |
| title_short | Application of cofactors in the regulation of microbial metabolism: A state of the art review |
| title_sort | application of cofactors in the regulation of microbial metabolism a state of the art review |
| topic | cofactors metabolic engineering regulatory strategies microbial cell factory biological manufacturing |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1145784/full |
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