Techniques of liver decellularization
Background: The growth of the number of people who need the liver transplant and the insufficiency of organ donors, have urged the advancement in bioengineering through the development of new therapeutic strategies which involve generation of functional artificial organs, obtained by the decellulari...
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| Format: | Article |
| Language: | English |
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Scientific Medical Association of Moldova
2018-12-01
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| Series: | The Moldovan Medical Journal |
| Subjects: | |
| Online Access: | http://moldmedjournal.md/wp-content/uploads/2018/12/moldmedjournal-2018-61-4-jian-et-al-full-text.pdf |
| _version_ | 1828845260326830080 |
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| author | Mariana Jian Vitalie Cobzac Ivan Moghildea Victor Popescu Viorel Nacu |
| author_facet | Mariana Jian Vitalie Cobzac Ivan Moghildea Victor Popescu Viorel Nacu |
| author_sort | Mariana Jian |
| collection | DOAJ |
| description | Background: The growth of the number of people who need the liver transplant and the insufficiency of organ donors, have urged the advancement in bioengineering through the development of new therapeutic strategies which involve generation of functional artificial organs, obtained by the decellularization technology and creation of extracellular matrix and their subsequent recellularization. Material and methods: Rat livers (n = 9) served as the object of this study which were subjected to decellularization with sodium dodecyl sulfate (SDS) solution 0.1 and 0.5% and the combination of SDS 0.1 to 0.5% and anticoagulant. Subsequently, the extraction of nucleic acids was performed according to the protocol QIAamp Blood Mini Kit (2003). The histological analysis was performed with haematoxylin-eosin (H-E) and the quantification of hydroxyproline via spectrophotometric method. Results: After the liver tissue decellularization we obtained the matrix of decellularized liver. The genetical, biochemical and histological analysis revealed a better decellularization by the combined method versus the method with SDS solution only. Conclusions: The quantification of nucleic acids content, hydroxyproline and the histological analysis of decellularized matrix with anticoagulant and detergent SDS method, suggested a more efficient decellularization of liver tissue segments and we achieved a decellularized bioconstruction for recellularization. |
| first_indexed | 2024-12-12T21:23:24Z |
| format | Article |
| id | doaj.art-d60d65ea6e3d45d1baec8ccb2ff036cc |
| institution | Directory Open Access Journal |
| issn | 2537-6373 2537-6381 |
| language | English |
| last_indexed | 2024-12-12T21:23:24Z |
| publishDate | 2018-12-01 |
| publisher | Scientific Medical Association of Moldova |
| record_format | Article |
| series | The Moldovan Medical Journal |
| spelling | doaj.art-d60d65ea6e3d45d1baec8ccb2ff036cc2022-12-22T00:11:31ZengScientific Medical Association of MoldovaThe Moldovan Medical Journal2537-63732537-63812018-12-01614212410.5281/zenodo.2222303Techniques of liver decellularizationMariana Jian0Vitalie Cobzac1Ivan Moghildea2Victor Popescu3Viorel Nacu4Tissue Engineering and Cells Cultures Laboratory, Nicolae Testemitsanu State University of Medicine and Pharmacy, Chisinau, the Republic of MoldovaTissue Engineering and Cells Cultures Laboratory, Nicolae Testemitsanu State University of Medicine and Pharmacy, Chisinau, the Republic of MoldovaTissue Engineering and Cells Cultures Laboratory, Nicolae Testemitsanu State University of Medicine and Pharmacy, Chisinau, the Republic of MoldovaTissue Engineering and Cells Cultures Laboratory, Nicolae Testemitsanu State University of Medicine and Pharmacy, Chisinau, the Republic of MoldovaTissue Engineering and Cells Cultures Laboratory, Nicolae Testemitsanu State University of Medicine and Pharmacy, Chisinau, the Republic of MoldovaBackground: The growth of the number of people who need the liver transplant and the insufficiency of organ donors, have urged the advancement in bioengineering through the development of new therapeutic strategies which involve generation of functional artificial organs, obtained by the decellularization technology and creation of extracellular matrix and their subsequent recellularization. Material and methods: Rat livers (n = 9) served as the object of this study which were subjected to decellularization with sodium dodecyl sulfate (SDS) solution 0.1 and 0.5% and the combination of SDS 0.1 to 0.5% and anticoagulant. Subsequently, the extraction of nucleic acids was performed according to the protocol QIAamp Blood Mini Kit (2003). The histological analysis was performed with haematoxylin-eosin (H-E) and the quantification of hydroxyproline via spectrophotometric method. Results: After the liver tissue decellularization we obtained the matrix of decellularized liver. The genetical, biochemical and histological analysis revealed a better decellularization by the combined method versus the method with SDS solution only. Conclusions: The quantification of nucleic acids content, hydroxyproline and the histological analysis of decellularized matrix with anticoagulant and detergent SDS method, suggested a more efficient decellularization of liver tissue segments and we achieved a decellularized bioconstruction for recellularization.http://moldmedjournal.md/wp-content/uploads/2018/12/moldmedjournal-2018-61-4-jian-et-al-full-text.pdfdecellularizationrecellularizationliver matrix |
| spellingShingle | Mariana Jian Vitalie Cobzac Ivan Moghildea Victor Popescu Viorel Nacu Techniques of liver decellularization The Moldovan Medical Journal decellularization recellularization liver matrix |
| title | Techniques of liver decellularization |
| title_full | Techniques of liver decellularization |
| title_fullStr | Techniques of liver decellularization |
| title_full_unstemmed | Techniques of liver decellularization |
| title_short | Techniques of liver decellularization |
| title_sort | techniques of liver decellularization |
| topic | decellularization recellularization liver matrix |
| url | http://moldmedjournal.md/wp-content/uploads/2018/12/moldmedjournal-2018-61-4-jian-et-al-full-text.pdf |
| work_keys_str_mv | AT marianajian techniquesofliverdecellularization AT vitaliecobzac techniquesofliverdecellularization AT ivanmoghildea techniquesofliverdecellularization AT victorpopescu techniquesofliverdecellularization AT viorelnacu techniquesofliverdecellularization |