TranSPHIRE: automated and feedback-optimized on-the-fly processing for cryo-EM
High-throughput single particle cryo-EM, for instance in drug research, requires the automation of the single particle analysis workflow. Here, the authors present TranSPHIRE, a software package that allows the fully-automated, feedback-driven processing of cryo-EM datasets during data acquisition.
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2020-11-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-19513-2 |
| _version_ | 1818851527125630976 |
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| author | Markus Stabrin Fabian Schoenfeld Thorsten Wagner Sabrina Pospich Christos Gatsogiannis Stefan Raunser |
| author_facet | Markus Stabrin Fabian Schoenfeld Thorsten Wagner Sabrina Pospich Christos Gatsogiannis Stefan Raunser |
| author_sort | Markus Stabrin |
| collection | DOAJ |
| description | High-throughput single particle cryo-EM, for instance in drug research, requires the automation of the single particle analysis workflow. Here, the authors present TranSPHIRE, a software package that allows the fully-automated, feedback-driven processing of cryo-EM datasets during data acquisition. |
| first_indexed | 2024-12-19T07:06:26Z |
| format | Article |
| id | doaj.art-d60f1db43f614ca5b692b67d9a2a71c0 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-19T07:06:26Z |
| publishDate | 2020-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-d60f1db43f614ca5b692b67d9a2a71c02022-12-21T20:31:17ZengNature PortfolioNature Communications2041-17232020-11-0111111410.1038/s41467-020-19513-2TranSPHIRE: automated and feedback-optimized on-the-fly processing for cryo-EMMarkus Stabrin0Fabian Schoenfeld1Thorsten Wagner2Sabrina Pospich3Christos Gatsogiannis4Stefan Raunser5Department of Structural Biochemistry, Max Planck Institute of Molecular PhysiologyDepartment of Structural Biochemistry, Max Planck Institute of Molecular PhysiologyDepartment of Structural Biochemistry, Max Planck Institute of Molecular PhysiologyDepartment of Structural Biochemistry, Max Planck Institute of Molecular PhysiologyDepartment of Structural Biochemistry, Max Planck Institute of Molecular PhysiologyDepartment of Structural Biochemistry, Max Planck Institute of Molecular PhysiologyHigh-throughput single particle cryo-EM, for instance in drug research, requires the automation of the single particle analysis workflow. Here, the authors present TranSPHIRE, a software package that allows the fully-automated, feedback-driven processing of cryo-EM datasets during data acquisition.https://doi.org/10.1038/s41467-020-19513-2 |
| spellingShingle | Markus Stabrin Fabian Schoenfeld Thorsten Wagner Sabrina Pospich Christos Gatsogiannis Stefan Raunser TranSPHIRE: automated and feedback-optimized on-the-fly processing for cryo-EM Nature Communications |
| title | TranSPHIRE: automated and feedback-optimized on-the-fly processing for cryo-EM |
| title_full | TranSPHIRE: automated and feedback-optimized on-the-fly processing for cryo-EM |
| title_fullStr | TranSPHIRE: automated and feedback-optimized on-the-fly processing for cryo-EM |
| title_full_unstemmed | TranSPHIRE: automated and feedback-optimized on-the-fly processing for cryo-EM |
| title_short | TranSPHIRE: automated and feedback-optimized on-the-fly processing for cryo-EM |
| title_sort | transphire automated and feedback optimized on the fly processing for cryo em |
| url | https://doi.org/10.1038/s41467-020-19513-2 |
| work_keys_str_mv | AT markusstabrin transphireautomatedandfeedbackoptimizedontheflyprocessingforcryoem AT fabianschoenfeld transphireautomatedandfeedbackoptimizedontheflyprocessingforcryoem AT thorstenwagner transphireautomatedandfeedbackoptimizedontheflyprocessingforcryoem AT sabrinapospich transphireautomatedandfeedbackoptimizedontheflyprocessingforcryoem AT christosgatsogiannis transphireautomatedandfeedbackoptimizedontheflyprocessingforcryoem AT stefanraunser transphireautomatedandfeedbackoptimizedontheflyprocessingforcryoem |