Effects of <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> IU100 on Immunomodulation and Gut Microbiota in Immunosuppressed Mice
Probiotics are live microorganisms with immunomodulatory effects in a strain-specific and dose-dependent manner. <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> IU100 is a new probiotic strain isolated from healthy adults. This study aimed to evaluate the effects of IU10...
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MDPI AG
2024-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/12/3/493 |
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| author | Limian Zhou Xindi Yin Bing Fang Jingjing He Jing Zhan Xiaoxu Zhang Ran Wang |
| author_facet | Limian Zhou Xindi Yin Bing Fang Jingjing He Jing Zhan Xiaoxu Zhang Ran Wang |
| author_sort | Limian Zhou |
| collection | DOAJ |
| description | Probiotics are live microorganisms with immunomodulatory effects in a strain-specific and dose-dependent manner. <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> IU100 is a new probiotic strain isolated from healthy adults. This study aimed to evaluate the effects of IU100 on cyclophosphamide (CTX)-induced immunosuppression in mice. The results showed that IU100 significantly ameliorated CTX-induced decreases in body weight and immune organ indices. The promoted delayed-type hypersensitivity, serum hemolysins and immunoglobulin (IgA, IgG and IgM) levels after IU100 treatment indicated its enhancing role in cellular and humoral immunity. In addition, oral administration of IU100 increased serum cytokine (IL-1β, IL-2, IL-4, IL-6, IFN-γ, TNF-α) levels dose-dependently, which are associated with CTX-induced shifts in the Th1/Th2 balance. The probiotic IU100 also modulated the composition of gut microbiota by reducing the Firmicutes/Bacteroidetes ratio; increasing beneficial <i>Muribaculaceae</i> and the <i>Lachnospiraceae</i> NK4A136 group; and inhibiting harmful <i>Clostridium sensu stricto</i> 1, <i>Faecalibaculum</i> and <i>Staphylococcus</i> at the genus level. The above genera were found to be correlated with serum cytokines and antibody levels. These findings suggest that IU100 effectively enhances the immune function of immunosuppressed mice, induced by CTX, by regulating gut microbiota. |
| first_indexed | 2024-04-24T18:00:54Z |
| format | Article |
| id | doaj.art-d61107ca8b9c4d5a88060ed745cb50c3 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-04-24T18:00:54Z |
| publishDate | 2024-02-01 |
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| series | Microorganisms |
| spelling | doaj.art-d61107ca8b9c4d5a88060ed745cb50c32024-03-27T13:55:37ZengMDPI AGMicroorganisms2076-26072024-02-0112349310.3390/microorganisms12030493Effects of <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> IU100 on Immunomodulation and Gut Microbiota in Immunosuppressed MiceLimian Zhou0Xindi Yin1Bing Fang2Jingjing He3Jing Zhan4Xiaoxu Zhang5Ran Wang6Key Laboratory of Functional Dairy, Department of Nutrition and Health, China Agricultural University, Beijing 100190, ChinaKey Laboratory of Functional Dairy, Department of Nutrition and Health, China Agricultural University, Beijing 100190, ChinaKey Laboratory of Functional Dairy, Department of Nutrition and Health, China Agricultural University, Beijing 100190, ChinaKey Laboratory of Functional Dairy, Department of Nutrition and Health, China Agricultural University, Beijing 100190, ChinaKey Laboratory of Functional Dairy, Department of Nutrition and Health, China Agricultural University, Beijing 100190, ChinaKey Laboratory of Functional Dairy, Department of Nutrition and Health, China Agricultural University, Beijing 100190, ChinaKey Laboratory of Functional Dairy, Department of Nutrition and Health, China Agricultural University, Beijing 100190, ChinaProbiotics are live microorganisms with immunomodulatory effects in a strain-specific and dose-dependent manner. <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> IU100 is a new probiotic strain isolated from healthy adults. This study aimed to evaluate the effects of IU100 on cyclophosphamide (CTX)-induced immunosuppression in mice. The results showed that IU100 significantly ameliorated CTX-induced decreases in body weight and immune organ indices. The promoted delayed-type hypersensitivity, serum hemolysins and immunoglobulin (IgA, IgG and IgM) levels after IU100 treatment indicated its enhancing role in cellular and humoral immunity. In addition, oral administration of IU100 increased serum cytokine (IL-1β, IL-2, IL-4, IL-6, IFN-γ, TNF-α) levels dose-dependently, which are associated with CTX-induced shifts in the Th1/Th2 balance. The probiotic IU100 also modulated the composition of gut microbiota by reducing the Firmicutes/Bacteroidetes ratio; increasing beneficial <i>Muribaculaceae</i> and the <i>Lachnospiraceae</i> NK4A136 group; and inhibiting harmful <i>Clostridium sensu stricto</i> 1, <i>Faecalibaculum</i> and <i>Staphylococcus</i> at the genus level. The above genera were found to be correlated with serum cytokines and antibody levels. These findings suggest that IU100 effectively enhances the immune function of immunosuppressed mice, induced by CTX, by regulating gut microbiota.https://www.mdpi.com/2076-2607/12/3/493<i>Bifidobacterium animalis</i> subsp. <i>lactis</i> IU100cyclophosphamidegut microbiotaimmunosuppressionimmunomodulationprobiotic |
| spellingShingle | Limian Zhou Xindi Yin Bing Fang Jingjing He Jing Zhan Xiaoxu Zhang Ran Wang Effects of <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> IU100 on Immunomodulation and Gut Microbiota in Immunosuppressed Mice Microorganisms <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> IU100 cyclophosphamide gut microbiota immunosuppression immunomodulation probiotic |
| title | Effects of <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> IU100 on Immunomodulation and Gut Microbiota in Immunosuppressed Mice |
| title_full | Effects of <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> IU100 on Immunomodulation and Gut Microbiota in Immunosuppressed Mice |
| title_fullStr | Effects of <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> IU100 on Immunomodulation and Gut Microbiota in Immunosuppressed Mice |
| title_full_unstemmed | Effects of <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> IU100 on Immunomodulation and Gut Microbiota in Immunosuppressed Mice |
| title_short | Effects of <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> IU100 on Immunomodulation and Gut Microbiota in Immunosuppressed Mice |
| title_sort | effects of i bifidobacterium animalis i subsp i lactis i iu100 on immunomodulation and gut microbiota in immunosuppressed mice |
| topic | <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> IU100 cyclophosphamide gut microbiota immunosuppression immunomodulation probiotic |
| url | https://www.mdpi.com/2076-2607/12/3/493 |
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