Quantum dot nanobead immunochromatographic assay based on bispecific monoclonal antibody for the simultaneous detection of aflatoxin B1 and amantadine
A bispecific monoclonal antibody (BsMAb) that can simultaneously recognize aflatoxin B1 (AFB1) and amantadine (AMD) was prepared. Quantum dot nanobead immunochromatographic assay (QB-ICA) based on the BsMAb was developed for the simultaneous detection of AFB1 and AMD in four feed samples (suckling p...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Food and Agricultural Immunology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/09540105.2022.2080188 |
| _version_ | 1811242595774365696 |
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| author | Kezhuo Zhang Huadong Cai Min Lu Daixian Wei Jiaqi Yin Nengshui Ding Weihua Lai Juan Peng |
| author_facet | Kezhuo Zhang Huadong Cai Min Lu Daixian Wei Jiaqi Yin Nengshui Ding Weihua Lai Juan Peng |
| author_sort | Kezhuo Zhang |
| collection | DOAJ |
| description | A bispecific monoclonal antibody (BsMAb) that can simultaneously recognize aflatoxin B1 (AFB1) and amantadine (AMD) was prepared. Quantum dot nanobead immunochromatographic assay (QB-ICA) based on the BsMAb was developed for the simultaneous detection of AFB1 and AMD in four feed samples (suckling pig feed, piglet feed, sow feed, and compound feed for laying ducks). Under optimal experimental conditions, the LOD values of QB-ICA for AFB1 in four feed samples were 0.188, 0.221, 0.233, and 0.306 μg/kg, respectively. The LOD values of QB-ICA for AMD in the same feed samples were 0.125, 0.187, 0.076, and 0.191 μg/kg, respectively. The recovery rates of QB-ICA for AFB1 and AMD ranged from 91.55% to 150.87% and from 91.62% to 138.42%, respectively. The variation coefficients were all less than 15%. The proposed QB-ICA had high reliability and specificity for the simultaneous detection of AFB1 and AMD, providing a platform for simultaneous detection of hazardous substances. |
| first_indexed | 2024-04-12T13:53:28Z |
| format | Article |
| id | doaj.art-d6113eac5c36450989fe3859c596dfff |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-04-12T13:53:28Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-d6113eac5c36450989fe3859c596dfff2022-12-22T03:30:27ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432022-12-0133140341810.1080/09540105.2022.2080188Quantum dot nanobead immunochromatographic assay based on bispecific monoclonal antibody for the simultaneous detection of aflatoxin B1 and amantadineKezhuo Zhang0Huadong Cai1Min Lu2Daixian Wei3Jiaqi Yin4Nengshui Ding5Weihua Lai6Juan Peng7State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, People’s Republic of ChinaGanzhou City Animal Husbandry Development & Animal Epidemic Diseases Prevention and Control Center, Ganzhou, People’s Republic of ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, People’s Republic of ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, People’s Republic of ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, People’s Republic of ChinaState Key Laboratory of Food Safety Technology for Meat Products, Xiamen, People’s Republic of ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, People’s Republic of ChinaSchool of Food Science, Nanchang University, Nanchang, People’s Republic of ChinaA bispecific monoclonal antibody (BsMAb) that can simultaneously recognize aflatoxin B1 (AFB1) and amantadine (AMD) was prepared. Quantum dot nanobead immunochromatographic assay (QB-ICA) based on the BsMAb was developed for the simultaneous detection of AFB1 and AMD in four feed samples (suckling pig feed, piglet feed, sow feed, and compound feed for laying ducks). Under optimal experimental conditions, the LOD values of QB-ICA for AFB1 in four feed samples were 0.188, 0.221, 0.233, and 0.306 μg/kg, respectively. The LOD values of QB-ICA for AMD in the same feed samples were 0.125, 0.187, 0.076, and 0.191 μg/kg, respectively. The recovery rates of QB-ICA for AFB1 and AMD ranged from 91.55% to 150.87% and from 91.62% to 138.42%, respectively. The variation coefficients were all less than 15%. The proposed QB-ICA had high reliability and specificity for the simultaneous detection of AFB1 and AMD, providing a platform for simultaneous detection of hazardous substances.https://www.tandfonline.com/doi/10.1080/09540105.2022.2080188Bispecific monoclonal antibodyaflatoxin B1amantadineimmunochromatographic assayquantum dot fluorescent nanobead |
| spellingShingle | Kezhuo Zhang Huadong Cai Min Lu Daixian Wei Jiaqi Yin Nengshui Ding Weihua Lai Juan Peng Quantum dot nanobead immunochromatographic assay based on bispecific monoclonal antibody for the simultaneous detection of aflatoxin B1 and amantadine Food and Agricultural Immunology Bispecific monoclonal antibody aflatoxin B1 amantadine immunochromatographic assay quantum dot fluorescent nanobead |
| title | Quantum dot nanobead immunochromatographic assay based on bispecific monoclonal antibody for the simultaneous detection of aflatoxin B1 and amantadine |
| title_full | Quantum dot nanobead immunochromatographic assay based on bispecific monoclonal antibody for the simultaneous detection of aflatoxin B1 and amantadine |
| title_fullStr | Quantum dot nanobead immunochromatographic assay based on bispecific monoclonal antibody for the simultaneous detection of aflatoxin B1 and amantadine |
| title_full_unstemmed | Quantum dot nanobead immunochromatographic assay based on bispecific monoclonal antibody for the simultaneous detection of aflatoxin B1 and amantadine |
| title_short | Quantum dot nanobead immunochromatographic assay based on bispecific monoclonal antibody for the simultaneous detection of aflatoxin B1 and amantadine |
| title_sort | quantum dot nanobead immunochromatographic assay based on bispecific monoclonal antibody for the simultaneous detection of aflatoxin b1 and amantadine |
| topic | Bispecific monoclonal antibody aflatoxin B1 amantadine immunochromatographic assay quantum dot fluorescent nanobead |
| url | https://www.tandfonline.com/doi/10.1080/09540105.2022.2080188 |
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