Endothelial identity not found – Beyond passage 38, commercial cardiac microvascular endothelial cells do not express CD31 and VE-cadherin
Few immortalized cardiac microvascular endothelial cell (CMEC) lines are available, particularly mouse lines. We purchased the CLU510 mCMEC line (Cedarlane), isolated by fluorescence-activated cell sorting for CD31 and VE-cadherin. The cell line has been used in previous studies, although none repor...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | Journal of Molecular and Cellular Cardiology Plus |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772976124000114 |
| _version_ | 1797249111621107712 |
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| author | Sarah Hilderink Jolanda van der Velden Diederik W.D. Kuster |
| author_facet | Sarah Hilderink Jolanda van der Velden Diederik W.D. Kuster |
| author_sort | Sarah Hilderink |
| collection | DOAJ |
| description | Few immortalized cardiac microvascular endothelial cell (CMEC) lines are available, particularly mouse lines. We purchased the CLU510 mCMEC line (Cedarlane), isolated by fluorescence-activated cell sorting for CD31 and VE-cadherin. The cell line has been used in previous studies, although none report CD31 or VE-cadherin expression. We analyzed endothelial profile of two vials of passage 38 cells. CD31 and VE-cadherin mRNA were hardly expressed in mCMECs compared to primary mouse lung ECs. CD31 and VE-cadherin protein levels were also negligible compared to multiple EC lines. Thus, CLU510 mCMECs beyond P38 do not harbor an endothelial phenotype. Caution should be warranted when using commercial cells and journals should carefully consider the validity of results when essential characterization of cell lines is omitted. |
| first_indexed | 2024-04-24T20:25:17Z |
| format | Article |
| id | doaj.art-d61490114afc43a083f56b05a38e9934 |
| institution | Directory Open Access Journal |
| issn | 2772-9761 |
| language | English |
| last_indexed | 2024-04-24T20:25:17Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Molecular and Cellular Cardiology Plus |
| spelling | doaj.art-d61490114afc43a083f56b05a38e99342024-03-22T05:41:23ZengElsevierJournal of Molecular and Cellular Cardiology Plus2772-97612024-06-018100071Endothelial identity not found – Beyond passage 38, commercial cardiac microvascular endothelial cells do not express CD31 and VE-cadherinSarah Hilderink0Jolanda van der Velden1Diederik W.D. Kuster2Amsterdam UMC location Vrije Universiteit Amsterdam, Physiology, De Boelelaan 1118, Amsterdam, the Netherlands; Amsterdam Cardiovascular Sciences, Heart Failure & Arrhythmias, Amsterdam, the NetherlandsAmsterdam UMC location Vrije Universiteit Amsterdam, Physiology, De Boelelaan 1118, Amsterdam, the Netherlands; Amsterdam Cardiovascular Sciences, Heart Failure & Arrhythmias, Amsterdam, the NetherlandsCorresponding author at: Physiology, Amsterdam UMC, De Boelelaan 1118, 1081 HZ Amsterdam, the Netherlands.; Amsterdam UMC location Vrije Universiteit Amsterdam, Physiology, De Boelelaan 1118, Amsterdam, the Netherlands; Amsterdam Cardiovascular Sciences, Heart Failure & Arrhythmias, Amsterdam, the NetherlandsFew immortalized cardiac microvascular endothelial cell (CMEC) lines are available, particularly mouse lines. We purchased the CLU510 mCMEC line (Cedarlane), isolated by fluorescence-activated cell sorting for CD31 and VE-cadherin. The cell line has been used in previous studies, although none report CD31 or VE-cadherin expression. We analyzed endothelial profile of two vials of passage 38 cells. CD31 and VE-cadherin mRNA were hardly expressed in mCMECs compared to primary mouse lung ECs. CD31 and VE-cadherin protein levels were also negligible compared to multiple EC lines. Thus, CLU510 mCMECs beyond P38 do not harbor an endothelial phenotype. Caution should be warranted when using commercial cells and journals should carefully consider the validity of results when essential characterization of cell lines is omitted.http://www.sciencedirect.com/science/article/pii/S2772976124000114Cardiac microvascular endothelial cellsValidationCommercial cell linesReproducibility |
| spellingShingle | Sarah Hilderink Jolanda van der Velden Diederik W.D. Kuster Endothelial identity not found – Beyond passage 38, commercial cardiac microvascular endothelial cells do not express CD31 and VE-cadherin Journal of Molecular and Cellular Cardiology Plus Cardiac microvascular endothelial cells Validation Commercial cell lines Reproducibility |
| title | Endothelial identity not found – Beyond passage 38, commercial cardiac microvascular endothelial cells do not express CD31 and VE-cadherin |
| title_full | Endothelial identity not found – Beyond passage 38, commercial cardiac microvascular endothelial cells do not express CD31 and VE-cadherin |
| title_fullStr | Endothelial identity not found – Beyond passage 38, commercial cardiac microvascular endothelial cells do not express CD31 and VE-cadherin |
| title_full_unstemmed | Endothelial identity not found – Beyond passage 38, commercial cardiac microvascular endothelial cells do not express CD31 and VE-cadherin |
| title_short | Endothelial identity not found – Beyond passage 38, commercial cardiac microvascular endothelial cells do not express CD31 and VE-cadherin |
| title_sort | endothelial identity not found beyond passage 38 commercial cardiac microvascular endothelial cells do not express cd31 and ve cadherin |
| topic | Cardiac microvascular endothelial cells Validation Commercial cell lines Reproducibility |
| url | http://www.sciencedirect.com/science/article/pii/S2772976124000114 |
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