Secondary Somatic Embryogenesis in <i>Centaurium erythraea</i> Rafn
Somatic embryogenesis (SE) is a developmental process during which plant somatic cells, under suitable conditions, produce embryogenic cells that develop into somatic embryos (<b>se</b>). SE is the most important method for plant propagation in vitro, having both fundamental and applicat...
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MDPI AG
2021-01-01
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| Accès en ligne: | https://www.mdpi.com/2223-7747/10/2/199 |
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| author | Milica D. Bogdanović Katarina B. Ćuković Angelina R. Subotić Milan B. Dragićević Ana D. Simonović Biljana K. Filipović Slađana I. Todorović |
| author_facet | Milica D. Bogdanović Katarina B. Ćuković Angelina R. Subotić Milan B. Dragićević Ana D. Simonović Biljana K. Filipović Slađana I. Todorović |
| author_sort | Milica D. Bogdanović |
| collection | DOAJ |
| description | Somatic embryogenesis (SE) is a developmental process during which plant somatic cells, under suitable conditions, produce embryogenic cells that develop into somatic embryos (<b>se</b>). SE is the most important method for plant propagation in vitro, having both fundamental and applicative significance. SE can be induced from different tissues and organs, but when <b>se</b> are used as explants, the process is recognized as secondary or cyclic SE. We induced secondary SE in <i>Centaurium erythraea</i> by application of 2,4-dichlorophenoxyacetic acid (2,4-D) and <i>N</i>-(2-chloro-4-pyridyl)-<i>N</i>′-phenylurea (CPPU). A medium containing 0.1 mgL<sup>−1</sup> 2,4-D and 0.25 mgL<sup>−1</sup> CPPU was optimal in terms of the number of primary SE explants forming <b>se</b>, the number of well-developed <b>se</b> per explant, and morphological appearance of the obtained <b>se</b>. These concentrations allowed SE to progress through three cycles, whereas at higher concentrations of 0.2 mgL<sup>−1</sup> 2,4-D and 0.5 mgL<sup>−1</sup> CPPU, only two cycles were achieved. Histological analysis revealed that secondary <b>se</b> are formed both directly and indirectly. Secondary SE readily germinated and converted into plantlets. Induction of cyclic SE contributes to the conservation efforts of this endangered medicinal plant and expands the spectrum of in vitro developmental pathways described in centaury—an emerging model in developmental biology. |
| first_indexed | 2024-03-09T04:02:58Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T04:02:58Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-d6161fb1c03b47fd87a410ca330ce6aa2023-12-03T14:10:55ZengMDPI AGPlants2223-77472021-01-0110219910.3390/plants10020199Secondary Somatic Embryogenesis in <i>Centaurium erythraea</i> RafnMilica D. Bogdanović0Katarina B. Ćuković1Angelina R. Subotić2Milan B. Dragićević3Ana D. Simonović4Biljana K. Filipović5Slađana I. Todorović6Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”-National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, SerbiaDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”-National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, SerbiaDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”-National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, SerbiaDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”-National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, SerbiaDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”-National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, SerbiaDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”-National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, SerbiaDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković”-National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, SerbiaSomatic embryogenesis (SE) is a developmental process during which plant somatic cells, under suitable conditions, produce embryogenic cells that develop into somatic embryos (<b>se</b>). SE is the most important method for plant propagation in vitro, having both fundamental and applicative significance. SE can be induced from different tissues and organs, but when <b>se</b> are used as explants, the process is recognized as secondary or cyclic SE. We induced secondary SE in <i>Centaurium erythraea</i> by application of 2,4-dichlorophenoxyacetic acid (2,4-D) and <i>N</i>-(2-chloro-4-pyridyl)-<i>N</i>′-phenylurea (CPPU). A medium containing 0.1 mgL<sup>−1</sup> 2,4-D and 0.25 mgL<sup>−1</sup> CPPU was optimal in terms of the number of primary SE explants forming <b>se</b>, the number of well-developed <b>se</b> per explant, and morphological appearance of the obtained <b>se</b>. These concentrations allowed SE to progress through three cycles, whereas at higher concentrations of 0.2 mgL<sup>−1</sup> 2,4-D and 0.5 mgL<sup>−1</sup> CPPU, only two cycles were achieved. Histological analysis revealed that secondary <b>se</b> are formed both directly and indirectly. Secondary SE readily germinated and converted into plantlets. Induction of cyclic SE contributes to the conservation efforts of this endangered medicinal plant and expands the spectrum of in vitro developmental pathways described in centaury—an emerging model in developmental biology.https://www.mdpi.com/2223-7747/10/2/199cyclic somatic embryogenesisdirect somatic embryogenesisindirect somatic embryogenesisleaf explanthistology2,4-D |
| spellingShingle | Milica D. Bogdanović Katarina B. Ćuković Angelina R. Subotić Milan B. Dragićević Ana D. Simonović Biljana K. Filipović Slađana I. Todorović Secondary Somatic Embryogenesis in <i>Centaurium erythraea</i> Rafn Plants cyclic somatic embryogenesis direct somatic embryogenesis indirect somatic embryogenesis leaf explant histology 2,4-D |
| title | Secondary Somatic Embryogenesis in <i>Centaurium erythraea</i> Rafn |
| title_full | Secondary Somatic Embryogenesis in <i>Centaurium erythraea</i> Rafn |
| title_fullStr | Secondary Somatic Embryogenesis in <i>Centaurium erythraea</i> Rafn |
| title_full_unstemmed | Secondary Somatic Embryogenesis in <i>Centaurium erythraea</i> Rafn |
| title_short | Secondary Somatic Embryogenesis in <i>Centaurium erythraea</i> Rafn |
| title_sort | secondary somatic embryogenesis in i centaurium erythraea i rafn |
| topic | cyclic somatic embryogenesis direct somatic embryogenesis indirect somatic embryogenesis leaf explant histology 2,4-D |
| url | https://www.mdpi.com/2223-7747/10/2/199 |
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