Analysis of the Prognostic Potential of Schlafen 11, Programmed Death Ligand 1, and Redox Status in Colorectal Cancer Patients
The Schlafen 11 (SLFN11) protein has recently emerged as pivotal in DNA damage conditions, with predictive potential for tumor response to cytotoxic chemotherapies. Recent discoveries also showed that the programmed death ligand 1 (PD-L1) protein can be found on malignant cells, providing an immune...
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MDPI AG
2023-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/20/15083 |
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| author | Marko Miladinov Jovana Rosic Katarina Eric Azra Guzonjic Jelenko Jelenkovic Natasa Bogavac-Stanojevic Ivan Dimitrijevic Jelena Kotur-Stevuljevic Goran Barisic |
| author_facet | Marko Miladinov Jovana Rosic Katarina Eric Azra Guzonjic Jelenko Jelenkovic Natasa Bogavac-Stanojevic Ivan Dimitrijevic Jelena Kotur-Stevuljevic Goran Barisic |
| author_sort | Marko Miladinov |
| collection | DOAJ |
| description | The Schlafen 11 (SLFN11) protein has recently emerged as pivotal in DNA damage conditions, with predictive potential for tumor response to cytotoxic chemotherapies. Recent discoveries also showed that the programmed death ligand 1 (PD-L1) protein can be found on malignant cells, providing an immune evasion mechanism exploited by different tumors. Additionally, excessive generation of free radicals, redox imbalance, and consequential DNA damage can affect intestinal cell homeostasis and lead to neoplastic transformation. Therefore, our study aimed to investigate the significance of SLFN11 and PD-L1 proteins and redox status parameters as prognostic biomarkers in CRC patients. This study included a total of 155 CRC patients. SLFN11 and PD-L1 serum levels were measured with ELISA and evaluated based on redox status parameters, sociodemographic and clinical characteristics, and survival. The following redox status parameters were investigated: spectrophotometrically measured superoxide dismutase (SOD), sulfhydryl (SH) groups, advanced oxidation protein products (AOPP), malondialdehyde (MDA), pro-oxidant–antioxidant balance (PAB), and superoxide anion (O<sub>2</sub><sup>•–</sup>). The prooxidative score, antioxidative score, and OXY-SCORE were also calculated. The results showed significantly shorter survival in patients with higher OXY-SCOREs and higher levels of serum SLFN11, while only histopathology-analysis-related factors showed significant prognostic value. OXY-SCORE and SLFN11 levels may harbor prognostic potential in CRC patients. |
| first_indexed | 2024-03-10T21:13:09Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T21:13:09Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d616c099900d4c14b74215cfcff530852023-11-19T16:41:23ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-10-0124201508310.3390/ijms242015083Analysis of the Prognostic Potential of Schlafen 11, Programmed Death Ligand 1, and Redox Status in Colorectal Cancer PatientsMarko Miladinov0Jovana Rosic1Katarina Eric2Azra Guzonjic3Jelenko Jelenkovic4Natasa Bogavac-Stanojevic5Ivan Dimitrijevic6Jelena Kotur-Stevuljevic7Goran Barisic8Clinic for Digestive Surgery—First Surgical Clinic, University Clinical Center of Serbia, 11000 Belgrade, SerbiaFaculty of Medicine, University of Belgrade, 11000 Belgrade, SerbiaDepartment of Pathology, University Clinical Center of Serbia, 11000 Belgrade, SerbiaFaculty of Pharmacy, University of Belgrade, 11000 Belgrade, SerbiaClinic for Digestive Surgery—First Surgical Clinic, University Clinical Center of Serbia, 11000 Belgrade, SerbiaFaculty of Pharmacy, University of Belgrade, 11000 Belgrade, SerbiaClinic for Digestive Surgery—First Surgical Clinic, University Clinical Center of Serbia, 11000 Belgrade, SerbiaFaculty of Pharmacy, University of Belgrade, 11000 Belgrade, SerbiaClinic for Digestive Surgery—First Surgical Clinic, University Clinical Center of Serbia, 11000 Belgrade, SerbiaThe Schlafen 11 (SLFN11) protein has recently emerged as pivotal in DNA damage conditions, with predictive potential for tumor response to cytotoxic chemotherapies. Recent discoveries also showed that the programmed death ligand 1 (PD-L1) protein can be found on malignant cells, providing an immune evasion mechanism exploited by different tumors. Additionally, excessive generation of free radicals, redox imbalance, and consequential DNA damage can affect intestinal cell homeostasis and lead to neoplastic transformation. Therefore, our study aimed to investigate the significance of SLFN11 and PD-L1 proteins and redox status parameters as prognostic biomarkers in CRC patients. This study included a total of 155 CRC patients. SLFN11 and PD-L1 serum levels were measured with ELISA and evaluated based on redox status parameters, sociodemographic and clinical characteristics, and survival. The following redox status parameters were investigated: spectrophotometrically measured superoxide dismutase (SOD), sulfhydryl (SH) groups, advanced oxidation protein products (AOPP), malondialdehyde (MDA), pro-oxidant–antioxidant balance (PAB), and superoxide anion (O<sub>2</sub><sup>•–</sup>). The prooxidative score, antioxidative score, and OXY-SCORE were also calculated. The results showed significantly shorter survival in patients with higher OXY-SCOREs and higher levels of serum SLFN11, while only histopathology-analysis-related factors showed significant prognostic value. OXY-SCORE and SLFN11 levels may harbor prognostic potential in CRC patients.https://www.mdpi.com/1422-0067/24/20/15083colorectal cancerredox statusoxidative stressSLFN11PD-L1biomarkers |
| spellingShingle | Marko Miladinov Jovana Rosic Katarina Eric Azra Guzonjic Jelenko Jelenkovic Natasa Bogavac-Stanojevic Ivan Dimitrijevic Jelena Kotur-Stevuljevic Goran Barisic Analysis of the Prognostic Potential of Schlafen 11, Programmed Death Ligand 1, and Redox Status in Colorectal Cancer Patients International Journal of Molecular Sciences colorectal cancer redox status oxidative stress SLFN11 PD-L1 biomarkers |
| title | Analysis of the Prognostic Potential of Schlafen 11, Programmed Death Ligand 1, and Redox Status in Colorectal Cancer Patients |
| title_full | Analysis of the Prognostic Potential of Schlafen 11, Programmed Death Ligand 1, and Redox Status in Colorectal Cancer Patients |
| title_fullStr | Analysis of the Prognostic Potential of Schlafen 11, Programmed Death Ligand 1, and Redox Status in Colorectal Cancer Patients |
| title_full_unstemmed | Analysis of the Prognostic Potential of Schlafen 11, Programmed Death Ligand 1, and Redox Status in Colorectal Cancer Patients |
| title_short | Analysis of the Prognostic Potential of Schlafen 11, Programmed Death Ligand 1, and Redox Status in Colorectal Cancer Patients |
| title_sort | analysis of the prognostic potential of schlafen 11 programmed death ligand 1 and redox status in colorectal cancer patients |
| topic | colorectal cancer redox status oxidative stress SLFN11 PD-L1 biomarkers |
| url | https://www.mdpi.com/1422-0067/24/20/15083 |
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