Cheap, rapid and efficient DNA extraction method to perform multilocus microsatellite genotyping on all Schistosoma mansoni stages
Schistosomes are endoparasites causing a serious human disease called schistosomiasis. The quantification of parasite genetic diversity is an essential component to understand the schistosomiasis epidemiology and disease transmission patterns. In this paper, we propose a novel assay for a rapid, low...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2008-08-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000500017 |
| _version_ | 1797715811723378688 |
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| author | S Beltran R Galinier JF Allienne J Boissier |
| author_facet | S Beltran R Galinier JF Allienne J Boissier |
| author_sort | S Beltran |
| collection | DOAJ |
| description | Schistosomes are endoparasites causing a serious human disease called schistosomiasis. The quantification of parasite genetic diversity is an essential component to understand the schistosomiasis epidemiology and disease transmission patterns. In this paper, we propose a novel assay for a rapid, low costly and efficient DNA extraction method of egg, larval and adult stages of Schistosoma mansoni. One euro makes possible to perform 60,000 DNA extraction reactions at top speed (only 15 min of incubation and 5 handling steps). |
| first_indexed | 2024-03-12T08:12:17Z |
| format | Article |
| id | doaj.art-d61726b678f14307b5f0378f90e1bb83 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:12:17Z |
| publishDate | 2008-08-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-d61726b678f14307b5f0378f90e1bb832023-09-02T19:06:57ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602008-08-01103550150310.1590/S0074-02762008000500017Cheap, rapid and efficient DNA extraction method to perform multilocus microsatellite genotyping on all Schistosoma mansoni stagesS BeltranR GalinierJF AllienneJ BoissierSchistosomes are endoparasites causing a serious human disease called schistosomiasis. The quantification of parasite genetic diversity is an essential component to understand the schistosomiasis epidemiology and disease transmission patterns. In this paper, we propose a novel assay for a rapid, low costly and efficient DNA extraction method of egg, larval and adult stages of Schistosoma mansoni. One euro makes possible to perform 60,000 DNA extraction reactions at top speed (only 15 min of incubation and 5 handling steps).http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000500017Schistosoma mansoniDNA extractionmicrosatellites |
| spellingShingle | S Beltran R Galinier JF Allienne J Boissier Cheap, rapid and efficient DNA extraction method to perform multilocus microsatellite genotyping on all Schistosoma mansoni stages Memorias do Instituto Oswaldo Cruz Schistosoma mansoni DNA extraction microsatellites |
| title | Cheap, rapid and efficient DNA extraction method to perform multilocus microsatellite genotyping on all Schistosoma mansoni stages |
| title_full | Cheap, rapid and efficient DNA extraction method to perform multilocus microsatellite genotyping on all Schistosoma mansoni stages |
| title_fullStr | Cheap, rapid and efficient DNA extraction method to perform multilocus microsatellite genotyping on all Schistosoma mansoni stages |
| title_full_unstemmed | Cheap, rapid and efficient DNA extraction method to perform multilocus microsatellite genotyping on all Schistosoma mansoni stages |
| title_short | Cheap, rapid and efficient DNA extraction method to perform multilocus microsatellite genotyping on all Schistosoma mansoni stages |
| title_sort | cheap rapid and efficient dna extraction method to perform multilocus microsatellite genotyping on all schistosoma mansoni stages |
| topic | Schistosoma mansoni DNA extraction microsatellites |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000500017 |
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