Nerve growth factor signalling in pathology and regeneration of human teeth
Abstract Nerve growth factor (NGF) is a key regulator of the development and differentiation of neuronal and non-neuronal cells. In the present study we examined the distribution of NGF and its low and high-affinity receptors, p75NTR and TrkA respectively, in permanent human teeth under normal and p...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-01455-3 |
| _version_ | 1818994271376637952 |
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| author | Thimios A. Mitsiadis Henry Magloire Pierfrancesco Pagella |
| author_facet | Thimios A. Mitsiadis Henry Magloire Pierfrancesco Pagella |
| author_sort | Thimios A. Mitsiadis |
| collection | DOAJ |
| description | Abstract Nerve growth factor (NGF) is a key regulator of the development and differentiation of neuronal and non-neuronal cells. In the present study we examined the distribution of NGF and its low and high-affinity receptors, p75NTR and TrkA respectively, in permanent human teeth under normal and pathological conditions. In intact functional teeth, NGF, p75NTR and TrkA are weakly expressed in dental pulp fibroblasts and odontoblasts that are responsible for dentine formation, while the NGF and p75NTR molecules are strongly expressed in nerve fibres innervating the dental pulp. In carious and injured teeth NGF and TrkA expression is upregulated in a selective manner in odontoblasts surrounding the injury sites, indicating a link between NGF signalling and dental tissue repair events. Accordingly, NGF and TrkA expression is strongly upregulated in cultured primary human dental mesenchymal cells during their differentiation into odontoblasts. Targeted release of NGF in cultured human tooth slices induced extensive axonal growth and migration of Schwann cells towards the NGF administration site. These results show that NGF signalling is strongly linked to pathological and regenerative processes in human teeth and suggest a potential role for this neurotrophic molecule in pulp regeneration. |
| first_indexed | 2024-12-20T20:55:18Z |
| format | Article |
| id | doaj.art-d617576779d349e08e7809a0c032b151 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-20T20:55:18Z |
| publishDate | 2017-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-d617576779d349e08e7809a0c032b1512022-12-21T19:26:51ZengNature PortfolioScientific Reports2045-23222017-05-017111410.1038/s41598-017-01455-3Nerve growth factor signalling in pathology and regeneration of human teethThimios A. Mitsiadis0Henry Magloire1Pierfrancesco Pagella2Orofacial Development and Regeneration, Institute of Oral Biology, Centre for Dental Medicine, Medical Faculty, University of ZurichInstitut de Génomique Fonctionnelle de Lyon, Ecole Normale Supérieure (ENS)Orofacial Development and Regeneration, Institute of Oral Biology, Centre for Dental Medicine, Medical Faculty, University of ZurichAbstract Nerve growth factor (NGF) is a key regulator of the development and differentiation of neuronal and non-neuronal cells. In the present study we examined the distribution of NGF and its low and high-affinity receptors, p75NTR and TrkA respectively, in permanent human teeth under normal and pathological conditions. In intact functional teeth, NGF, p75NTR and TrkA are weakly expressed in dental pulp fibroblasts and odontoblasts that are responsible for dentine formation, while the NGF and p75NTR molecules are strongly expressed in nerve fibres innervating the dental pulp. In carious and injured teeth NGF and TrkA expression is upregulated in a selective manner in odontoblasts surrounding the injury sites, indicating a link between NGF signalling and dental tissue repair events. Accordingly, NGF and TrkA expression is strongly upregulated in cultured primary human dental mesenchymal cells during their differentiation into odontoblasts. Targeted release of NGF in cultured human tooth slices induced extensive axonal growth and migration of Schwann cells towards the NGF administration site. These results show that NGF signalling is strongly linked to pathological and regenerative processes in human teeth and suggest a potential role for this neurotrophic molecule in pulp regeneration.https://doi.org/10.1038/s41598-017-01455-3 |
| spellingShingle | Thimios A. Mitsiadis Henry Magloire Pierfrancesco Pagella Nerve growth factor signalling in pathology and regeneration of human teeth Scientific Reports |
| title | Nerve growth factor signalling in pathology and regeneration of human teeth |
| title_full | Nerve growth factor signalling in pathology and regeneration of human teeth |
| title_fullStr | Nerve growth factor signalling in pathology and regeneration of human teeth |
| title_full_unstemmed | Nerve growth factor signalling in pathology and regeneration of human teeth |
| title_short | Nerve growth factor signalling in pathology and regeneration of human teeth |
| title_sort | nerve growth factor signalling in pathology and regeneration of human teeth |
| url | https://doi.org/10.1038/s41598-017-01455-3 |
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