Values of alkaline phosphathase and their isoenzyme profiles in patients with cancer in respect to bone and liver metastasis
Background: Alkaline phosphatase is a glycoprotein that catalyzes two kinds of chemical reactions: hydrolysis of phosphorus ester breaking P-O bonds and phospho-transfer reactions in which phosphoric group is transferred to an acceptor molecule. In the human body, ALP exists in multiple molecular...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Institute of Oncology, Sremska Kamenica, Serbia
2013-01-01
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| Series: | Archive of Oncology |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-7310/2013/0354-73101301014D.pdf |
| _version_ | 1818876966417203200 |
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| author | Ðokić-Lišanin Marina Pantović Vesna Jovanović Zorica Samardžić Goran Jurišić Vladimir |
| author_facet | Ðokić-Lišanin Marina Pantović Vesna Jovanović Zorica Samardžić Goran Jurišić Vladimir |
| author_sort | Ðokić-Lišanin Marina |
| collection | DOAJ |
| description | Background: Alkaline phosphatase is a glycoprotein that catalyzes two kinds of chemical reactions: hydrolysis of phosphorus ester breaking P-O bonds and phospho-transfer reactions in which phosphoric group is transferred to an acceptor molecule. In the human body, ALP exists in multiple molecular forms whose heterogeneity is partly due to genetic factors and partly to posttranslational modifications. The aim was to evaluate a total ALP activity and its isoforms in cancer patients with bone and liver metastasis in comparison to healthy controls. Methods: Human serum was collected from 20 healthy individuals, and 20 cancer patients with bone and liver metastases, with metastases confirmed by ultrasound, computerized tomography and a radiology scan. Determination of ALP was done by the endpoint spectrophotometric method. Isoenzymes were determined by heat inactivation method. Results: In cancer patients, the total ALP activity was significantly higher (p< 0.05) compared to healthy controls. In the sera of cancer patients with liver metastases, the remaining ALP activity was two-fold higher in comparison to bone metastases. Conclusion: Determination of ALP isoenzymes is important but a correct clinical interpretation in the context of other analyses is vital for a proper diagnosis of a disease. [Projekat Ministarstva nauke Republike Srbije, br.175056] |
| first_indexed | 2024-12-19T13:50:47Z |
| format | Article |
| id | doaj.art-d6194108f49d4a8d9b480bef80ba7033 |
| institution | Directory Open Access Journal |
| issn | 0354-7310 1450-9520 |
| language | English |
| last_indexed | 2024-12-19T13:50:47Z |
| publishDate | 2013-01-01 |
| publisher | Institute of Oncology, Sremska Kamenica, Serbia |
| record_format | Article |
| series | Archive of Oncology |
| spelling | doaj.art-d6194108f49d4a8d9b480bef80ba70332022-12-21T20:18:45ZengInstitute of Oncology, Sremska Kamenica, SerbiaArchive of Oncology0354-73101450-95202013-01-01211141610.2298/AOO1301014DValues of alkaline phosphathase and their isoenzyme profiles in patients with cancer in respect to bone and liver metastasisÐokić-Lišanin MarinaPantović VesnaJovanović ZoricaSamardžić GoranJurišić VladimirBackground: Alkaline phosphatase is a glycoprotein that catalyzes two kinds of chemical reactions: hydrolysis of phosphorus ester breaking P-O bonds and phospho-transfer reactions in which phosphoric group is transferred to an acceptor molecule. In the human body, ALP exists in multiple molecular forms whose heterogeneity is partly due to genetic factors and partly to posttranslational modifications. The aim was to evaluate a total ALP activity and its isoforms in cancer patients with bone and liver metastasis in comparison to healthy controls. Methods: Human serum was collected from 20 healthy individuals, and 20 cancer patients with bone and liver metastases, with metastases confirmed by ultrasound, computerized tomography and a radiology scan. Determination of ALP was done by the endpoint spectrophotometric method. Isoenzymes were determined by heat inactivation method. Results: In cancer patients, the total ALP activity was significantly higher (p< 0.05) compared to healthy controls. In the sera of cancer patients with liver metastases, the remaining ALP activity was two-fold higher in comparison to bone metastases. Conclusion: Determination of ALP isoenzymes is important but a correct clinical interpretation in the context of other analyses is vital for a proper diagnosis of a disease. [Projekat Ministarstva nauke Republike Srbije, br.175056]http://www.doiserbia.nb.rs/img/doi/0354-7310/2013/0354-73101301014D.pdfAlkaline PhosphataseIsoenzymesNeoplasmsNeoplasm MetastasisLiver NeoplasmsBone Neoplasms |
| spellingShingle | Ðokić-Lišanin Marina Pantović Vesna Jovanović Zorica Samardžić Goran Jurišić Vladimir Values of alkaline phosphathase and their isoenzyme profiles in patients with cancer in respect to bone and liver metastasis Archive of Oncology Alkaline Phosphatase Isoenzymes Neoplasms Neoplasm Metastasis Liver Neoplasms Bone Neoplasms |
| title | Values of alkaline phosphathase and their isoenzyme profiles in patients with cancer in respect to bone and liver metastasis |
| title_full | Values of alkaline phosphathase and their isoenzyme profiles in patients with cancer in respect to bone and liver metastasis |
| title_fullStr | Values of alkaline phosphathase and their isoenzyme profiles in patients with cancer in respect to bone and liver metastasis |
| title_full_unstemmed | Values of alkaline phosphathase and their isoenzyme profiles in patients with cancer in respect to bone and liver metastasis |
| title_short | Values of alkaline phosphathase and their isoenzyme profiles in patients with cancer in respect to bone and liver metastasis |
| title_sort | values of alkaline phosphathase and their isoenzyme profiles in patients with cancer in respect to bone and liver metastasis |
| topic | Alkaline Phosphatase Isoenzymes Neoplasms Neoplasm Metastasis Liver Neoplasms Bone Neoplasms |
| url | http://www.doiserbia.nb.rs/img/doi/0354-7310/2013/0354-73101301014D.pdf |
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