Industrial Hemp Clone Selection Method under LED Smart Farm Condition Based on CBD Production per Cubic Meter

Cannabis breeders are combining several genes to develop economically valuable fiber, seed, and medicinal hemp. This study analyzed the characteristics and selection of traits based on cannabidiol production of medicinal cannabis lines successfully grown under artificial light and nutrient solution...

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Main Authors: Byeong-Ryeol Ryu, Chang-Hyeug Kim, Tae-Hyung Kwon, Joon-Hee Han, Gyeong-Ju Gim, Md Jahirul Islam, Md Obyedul Kalam Azad, Md Hafizur Rahman, Md Soyel Rana, Jung-Dae Lim, Young-Seok Lim
Format: Article
Language:English
Published: MDPI AG 2022-07-01
Series:Agronomy
Subjects:
Online Access:https://www.mdpi.com/2073-4395/12/8/1809
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author Byeong-Ryeol Ryu
Chang-Hyeug Kim
Tae-Hyung Kwon
Joon-Hee Han
Gyeong-Ju Gim
Md Jahirul Islam
Md Obyedul Kalam Azad
Md Hafizur Rahman
Md Soyel Rana
Jung-Dae Lim
Young-Seok Lim
author_facet Byeong-Ryeol Ryu
Chang-Hyeug Kim
Tae-Hyung Kwon
Joon-Hee Han
Gyeong-Ju Gim
Md Jahirul Islam
Md Obyedul Kalam Azad
Md Hafizur Rahman
Md Soyel Rana
Jung-Dae Lim
Young-Seok Lim
author_sort Byeong-Ryeol Ryu
collection DOAJ
description Cannabis breeders are combining several genes to develop economically valuable fiber, seed, and medicinal hemp. This study analyzed the characteristics and selection of traits based on cannabidiol production of medicinal cannabis lines successfully grown under artificial light and nutrient solution cultivation conditions in smart farm conditions. Sixteen female plants were selected by seeding medical hemp F1 hybrid specimens obtained by randomly crossing Cherry Wine and native hemp from each country. The F1 generation was treated with 12 h light to induce flower differentiation. CBD production peaked on day 50 of the treatment, and this was selected as the harvesting day. All F1 hybrids were separated by leaf and inflorescence after collecting morphological data, and fresh and dry weights were measured. The CBD production of leaf and inflorescence per cubic meter was calculated. The CW21-5 line produced a total of 53.002 ± 0.228 g of CBD per cubic meter, the highest CBD producer. In addition, heatmap correlation analysis showed that most morphological data were not related to cannabinoid content. Principal Component Analysis (PCA) and Self-Organizing Map (SOM) analysis showed that CW21-5 is an arbitrary line that does not cluster with other lines, and the reason for its excellent CBD yield per cubic meter is that it has a narrow plant diameter and a high CBD content at the same time.
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spelling doaj.art-d61bb3163f6d400283fad68ea2a98c522023-12-01T23:17:45ZengMDPI AGAgronomy2073-43952022-07-01128180910.3390/agronomy12081809Industrial Hemp Clone Selection Method under LED Smart Farm Condition Based on CBD Production per Cubic MeterByeong-Ryeol Ryu0Chang-Hyeug Kim1Tae-Hyung Kwon2Joon-Hee Han3Gyeong-Ju Gim4Md Jahirul Islam5Md Obyedul Kalam Azad6Md Hafizur Rahman7Md Soyel Rana8Jung-Dae Lim9Young-Seok Lim10Department of Bio-Health Convergence, Kangwon National University, Chuncheon 24341, KoreaDepartment of Research and Development, Chuncheon Bioindustry Foundation, Chuncheon 24232, KoreaDepartment of Research and Development, Chuncheon Bioindustry Foundation, Chuncheon 24232, KoreaDepartment of Research and Development, Chuncheon Bioindustry Foundation, Chuncheon 24232, KoreaDepartment of Bio-Health Convergence, Kangwon National University, Chuncheon 24341, KoreaPhysiology and Sugar Chemistry Division, Bangladesh Sugarcrop Research Institute, Ishurdi, Pabna 6620, BangladeshDepartment of Bio-Health Convergence, Kangwon National University, Chuncheon 24341, KoreaDepartment of Bio-Health Convergence, Kangwon National University, Chuncheon 24341, KoreaDepartment of Bio-Health Convergence, Kangwon National University, Chuncheon 24341, KoreaDepartment of Bio-Health Convergence, Kangwon National University, Chuncheon 24341, KoreaDepartment of Bio-Health Convergence, Kangwon National University, Chuncheon 24341, KoreaCannabis breeders are combining several genes to develop economically valuable fiber, seed, and medicinal hemp. This study analyzed the characteristics and selection of traits based on cannabidiol production of medicinal cannabis lines successfully grown under artificial light and nutrient solution cultivation conditions in smart farm conditions. Sixteen female plants were selected by seeding medical hemp F1 hybrid specimens obtained by randomly crossing Cherry Wine and native hemp from each country. The F1 generation was treated with 12 h light to induce flower differentiation. CBD production peaked on day 50 of the treatment, and this was selected as the harvesting day. All F1 hybrids were separated by leaf and inflorescence after collecting morphological data, and fresh and dry weights were measured. The CBD production of leaf and inflorescence per cubic meter was calculated. The CW21-5 line produced a total of 53.002 ± 0.228 g of CBD per cubic meter, the highest CBD producer. In addition, heatmap correlation analysis showed that most morphological data were not related to cannabinoid content. Principal Component Analysis (PCA) and Self-Organizing Map (SOM) analysis showed that CW21-5 is an arbitrary line that does not cluster with other lines, and the reason for its excellent CBD yield per cubic meter is that it has a narrow plant diameter and a high CBD content at the same time.https://www.mdpi.com/2073-4395/12/8/1809<i>Cannabis sativa</i> L.hemphydroponic systembreedingcannabinoidcannabidiol
spellingShingle Byeong-Ryeol Ryu
Chang-Hyeug Kim
Tae-Hyung Kwon
Joon-Hee Han
Gyeong-Ju Gim
Md Jahirul Islam
Md Obyedul Kalam Azad
Md Hafizur Rahman
Md Soyel Rana
Jung-Dae Lim
Young-Seok Lim
Industrial Hemp Clone Selection Method under LED Smart Farm Condition Based on CBD Production per Cubic Meter
Agronomy
<i>Cannabis sativa</i> L.
hemp
hydroponic system
breeding
cannabinoid
cannabidiol
title Industrial Hemp Clone Selection Method under LED Smart Farm Condition Based on CBD Production per Cubic Meter
title_full Industrial Hemp Clone Selection Method under LED Smart Farm Condition Based on CBD Production per Cubic Meter
title_fullStr Industrial Hemp Clone Selection Method under LED Smart Farm Condition Based on CBD Production per Cubic Meter
title_full_unstemmed Industrial Hemp Clone Selection Method under LED Smart Farm Condition Based on CBD Production per Cubic Meter
title_short Industrial Hemp Clone Selection Method under LED Smart Farm Condition Based on CBD Production per Cubic Meter
title_sort industrial hemp clone selection method under led smart farm condition based on cbd production per cubic meter
topic <i>Cannabis sativa</i> L.
hemp
hydroponic system
breeding
cannabinoid
cannabidiol
url https://www.mdpi.com/2073-4395/12/8/1809
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