Anaerobic digestion of the microalga Spirulina at extreme alkaline conditions: biogas production, metagenome and metatranscriptome
A haloalkaline anaerobic microbial community obtained from soda lake sediments was used to inoculate anaerobic reactors for the production of methane rich biogas. The microalga Spirulina was successfully digested by the haloalkaline microbial consortium at alkaline conditions (pH 10, 2.0 M Na+). Con...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00597/full |
| _version_ | 1818538429120512000 |
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| author | Vimac eNolla-Ardevol Marc eStrous Marc eStrous Marc eStrous Halina Elisabeth Tegetmeyer Halina Elisabeth Tegetmeyer Halina Elisabeth Tegetmeyer |
| author_facet | Vimac eNolla-Ardevol Marc eStrous Marc eStrous Marc eStrous Halina Elisabeth Tegetmeyer Halina Elisabeth Tegetmeyer Halina Elisabeth Tegetmeyer |
| author_sort | Vimac eNolla-Ardevol |
| collection | DOAJ |
| description | A haloalkaline anaerobic microbial community obtained from soda lake sediments was used to inoculate anaerobic reactors for the production of methane rich biogas. The microalga Spirulina was successfully digested by the haloalkaline microbial consortium at alkaline conditions (pH 10, 2.0 M Na+). Continuous biogas production was observed and the obtained biogas was rich in methane, up to 96 %. Alkaline medium acted as a CO2 scrubber which resulted in low amounts of CO2 and no traces of H2S in the produced biogas. A hydraulic retention time of 15 days and 0.25 g Spirulina L-1 day-1 organic loading rate were identified as the optimal operational parameters. Metagenomics and metatranscriptomics analysis showed that the hydrolysis of the supplied substrate was mainly carried out by Bacteroidetes of the ML635J-40 aquatic group while the hydrogenotrophic pathway was the main producer of methane in a methanogenic community dominated by Methanocalculus. |
| first_indexed | 2024-12-11T21:28:51Z |
| format | Article |
| id | doaj.art-d61c567f841c4df58f00482102a66cf4 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T21:28:51Z |
| publishDate | 2015-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-d61c567f841c4df58f00482102a66cf42022-12-22T00:50:15ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2015-06-01610.3389/fmicb.2015.00597143041Anaerobic digestion of the microalga Spirulina at extreme alkaline conditions: biogas production, metagenome and metatranscriptomeVimac eNolla-Ardevol0Marc eStrous1Marc eStrous2Marc eStrous3Halina Elisabeth Tegetmeyer4Halina Elisabeth Tegetmeyer5Halina Elisabeth Tegetmeyer6Universität BielefeldUniversität BielefeldUniversity of CalgaryMax Planck Institute for Marine MicrobiologyUniversität BielefeldMax Planck Institute for Marine MicrobiologyAlfred Wegener Institute, Helmholtz Centre for Polar and Marine ResearchA haloalkaline anaerobic microbial community obtained from soda lake sediments was used to inoculate anaerobic reactors for the production of methane rich biogas. The microalga Spirulina was successfully digested by the haloalkaline microbial consortium at alkaline conditions (pH 10, 2.0 M Na+). Continuous biogas production was observed and the obtained biogas was rich in methane, up to 96 %. Alkaline medium acted as a CO2 scrubber which resulted in low amounts of CO2 and no traces of H2S in the produced biogas. A hydraulic retention time of 15 days and 0.25 g Spirulina L-1 day-1 organic loading rate were identified as the optimal operational parameters. Metagenomics and metatranscriptomics analysis showed that the hydrolysis of the supplied substrate was mainly carried out by Bacteroidetes of the ML635J-40 aquatic group while the hydrogenotrophic pathway was the main producer of methane in a methanogenic community dominated by Methanocalculus.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00597/fullMicroalgaeSpirulinaBiogasalkaline lakesHaloalkalineMethane rich |
| spellingShingle | Vimac eNolla-Ardevol Marc eStrous Marc eStrous Marc eStrous Halina Elisabeth Tegetmeyer Halina Elisabeth Tegetmeyer Halina Elisabeth Tegetmeyer Anaerobic digestion of the microalga Spirulina at extreme alkaline conditions: biogas production, metagenome and metatranscriptome Frontiers in Microbiology Microalgae Spirulina Biogas alkaline lakes Haloalkaline Methane rich |
| title | Anaerobic digestion of the microalga Spirulina at extreme alkaline conditions: biogas production, metagenome and metatranscriptome |
| title_full | Anaerobic digestion of the microalga Spirulina at extreme alkaline conditions: biogas production, metagenome and metatranscriptome |
| title_fullStr | Anaerobic digestion of the microalga Spirulina at extreme alkaline conditions: biogas production, metagenome and metatranscriptome |
| title_full_unstemmed | Anaerobic digestion of the microalga Spirulina at extreme alkaline conditions: biogas production, metagenome and metatranscriptome |
| title_short | Anaerobic digestion of the microalga Spirulina at extreme alkaline conditions: biogas production, metagenome and metatranscriptome |
| title_sort | anaerobic digestion of the microalga spirulina at extreme alkaline conditions biogas production metagenome and metatranscriptome |
| topic | Microalgae Spirulina Biogas alkaline lakes Haloalkaline Methane rich |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00597/full |
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