Inhibition of potato leafroll virus multiplication and systemic translocation by siRNA constructs against putative ATPase fold of movement protein
Abstract Viruses cause many severe plant diseases, resulting in immense losses of crop yield worldwide. Therefore, developing novel approaches to control plant viruses is crucial to meet the demands of a growing world population. Recently, RNA interference (RNAi) has been widely used to develop viru...
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| Formatua: | Artikulua |
| Hizkuntza: | English |
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Nature Portfolio
2020-12-01
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| Saila: | Scientific Reports |
| Sarrera elektronikoa: | https://doi.org/10.1038/s41598-020-78791-4 |
| _version_ | 1828971288624889856 |
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| author | Priyanka Kumari Jitesh Kumar Ravi Ranjan Kumar Mohammad Ansar Kumari Rajani Sunil Kumar Tushar Ranjan |
| author_facet | Priyanka Kumari Jitesh Kumar Ravi Ranjan Kumar Mohammad Ansar Kumari Rajani Sunil Kumar Tushar Ranjan |
| author_sort | Priyanka Kumari |
| collection | DOAJ |
| description | Abstract Viruses cause many severe plant diseases, resulting in immense losses of crop yield worldwide. Therefore, developing novel approaches to control plant viruses is crucial to meet the demands of a growing world population. Recently, RNA interference (RNAi) has been widely used to develop virus-resistant plants. Once genome replication and assembly of virion particles is completed inside the host plant, mature virions or sometimes naked viral genomes spread cell-to-cell through plasmodesmata by interacting with the virus-encoded movement protein (MP). We used the RNAi approach to suppress MP gene expression, which in turn prevented potato leafroll virus (PLRV) systemic infection in Solanum tuberosum cv. Khufri Ashoka. Potato plants agroinfiltrated with MP siRNA constructs exhibited no rolling symptoms upon PLRV infection, indicating that the silencing of MP gene expression is an efficient method for generating PLRV-resistant potato plants. Further, we identified novel ATPase motifs in MP that may be involved in DNA binding and translocation through plasmodesmata. We also showed that the ATPase activity of MP was stimulated in the presence of DNA/RNA. Overall, our findings provide a robust technology to generate PLRV-resistant potato plants, which can be extended to other species. Moreover, this approach also contributes to the study of genome translocation mechanisms of plant viruses. |
| first_indexed | 2024-12-14T13:07:18Z |
| format | Article |
| id | doaj.art-d61cbfd904e647a08b2b4be7cceea8e8 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T13:07:18Z |
| publishDate | 2020-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-d61cbfd904e647a08b2b4be7cceea8e82022-12-21T23:00:17ZengNature PortfolioScientific Reports2045-23222020-12-0110111110.1038/s41598-020-78791-4Inhibition of potato leafroll virus multiplication and systemic translocation by siRNA constructs against putative ATPase fold of movement proteinPriyanka Kumari0Jitesh Kumar1Ravi Ranjan Kumar2Mohammad Ansar3Kumari Rajani4Sunil Kumar5Tushar Ranjan6Department of Molecular Biology and Genetic Engineering, Bihar Agricultural UniversityDepartment of Molecular Biology and Genetic Engineering, Bihar Agricultural UniversityDepartment of Molecular Biology and Genetic Engineering, Bihar Agricultural UniversityDepartment of Plant Pathology, Bihar Agricultural UniversityDepartment of Seed Science and Technology, Bihar Agricultural UniversityKusuma School of Biological Sciences, Indian Institute of Technology DelhiDepartment of Molecular Biology and Genetic Engineering, Bihar Agricultural UniversityAbstract Viruses cause many severe plant diseases, resulting in immense losses of crop yield worldwide. Therefore, developing novel approaches to control plant viruses is crucial to meet the demands of a growing world population. Recently, RNA interference (RNAi) has been widely used to develop virus-resistant plants. Once genome replication and assembly of virion particles is completed inside the host plant, mature virions or sometimes naked viral genomes spread cell-to-cell through plasmodesmata by interacting with the virus-encoded movement protein (MP). We used the RNAi approach to suppress MP gene expression, which in turn prevented potato leafroll virus (PLRV) systemic infection in Solanum tuberosum cv. Khufri Ashoka. Potato plants agroinfiltrated with MP siRNA constructs exhibited no rolling symptoms upon PLRV infection, indicating that the silencing of MP gene expression is an efficient method for generating PLRV-resistant potato plants. Further, we identified novel ATPase motifs in MP that may be involved in DNA binding and translocation through plasmodesmata. We also showed that the ATPase activity of MP was stimulated in the presence of DNA/RNA. Overall, our findings provide a robust technology to generate PLRV-resistant potato plants, which can be extended to other species. Moreover, this approach also contributes to the study of genome translocation mechanisms of plant viruses.https://doi.org/10.1038/s41598-020-78791-4 |
| spellingShingle | Priyanka Kumari Jitesh Kumar Ravi Ranjan Kumar Mohammad Ansar Kumari Rajani Sunil Kumar Tushar Ranjan Inhibition of potato leafroll virus multiplication and systemic translocation by siRNA constructs against putative ATPase fold of movement protein Scientific Reports |
| title | Inhibition of potato leafroll virus multiplication and systemic translocation by siRNA constructs against putative ATPase fold of movement protein |
| title_full | Inhibition of potato leafroll virus multiplication and systemic translocation by siRNA constructs against putative ATPase fold of movement protein |
| title_fullStr | Inhibition of potato leafroll virus multiplication and systemic translocation by siRNA constructs against putative ATPase fold of movement protein |
| title_full_unstemmed | Inhibition of potato leafroll virus multiplication and systemic translocation by siRNA constructs against putative ATPase fold of movement protein |
| title_short | Inhibition of potato leafroll virus multiplication and systemic translocation by siRNA constructs against putative ATPase fold of movement protein |
| title_sort | inhibition of potato leafroll virus multiplication and systemic translocation by sirna constructs against putative atpase fold of movement protein |
| url | https://doi.org/10.1038/s41598-020-78791-4 |
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