Comparisons in temperature and photoperiodic-dependent diapause induction between domestic and wild mulberry silkworms
Abstract The bivoltine strain of the domestic silkworm, Bombyx mori, has two generations per year. It shows a facultative diapause phenotype determined by environmental conditions, including photoperiod and temperature, and nutrient conditions during embryonic and larval development of the mother. H...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-87590-4 |
| _version_ | 1818684994936111104 |
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| author | Takeshi Yokoyama Shigeru Saito Misato Shimoda Masakazu Kobayashi Yoko Takasu Hideki Sezutsu Yoshiomi Kato Makoto Tominaga Akira Mizoguchi Kunihiro Shiomi |
| author_facet | Takeshi Yokoyama Shigeru Saito Misato Shimoda Masakazu Kobayashi Yoko Takasu Hideki Sezutsu Yoshiomi Kato Makoto Tominaga Akira Mizoguchi Kunihiro Shiomi |
| author_sort | Takeshi Yokoyama |
| collection | DOAJ |
| description | Abstract The bivoltine strain of the domestic silkworm, Bombyx mori, has two generations per year. It shows a facultative diapause phenotype determined by environmental conditions, including photoperiod and temperature, and nutrient conditions during embryonic and larval development of the mother. However, it remains unclear how the environmental signals received during development are selectively utilized as cues to determine alternative diapause phenotypes. We performed a comparative analysis between the Kosetsu strain of B. mori and a Japanese population of the wild mulberry silkworm B. mandarina concerning the hierarchical molecular mechanisms in diapause induction. Our results showed that for the Kosetsu, temperature signals during the mother’s embryonic development predominantly affected diapause determination through the thermosensitive transient receptor potential ankyrin 1 (TRPA1) and diapause hormone (DH) signaling pathways. However, embryonic diapause in B. mandarina was photoperiod-dependent, although the DH signaling pathway and thermal sensitivity of TRPA1 were conserved within both species. Based on these findings, we hypothesize that TRPA1-activated signals are strongly linked to the signaling pathway participating in diapause induction in Kosetsu to selectively utilize the temperature information as the cue because temperature-dependent induction was replaced by photoperiodic induction in the TRPA1 knockout mutant. |
| first_indexed | 2024-12-17T10:59:29Z |
| format | Article |
| id | doaj.art-d61d33c5a0534531aacecc95e9577350 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-17T10:59:29Z |
| publishDate | 2021-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-d61d33c5a0534531aacecc95e95773502022-12-21T21:51:44ZengNature PortfolioScientific Reports2045-23222021-04-011111910.1038/s41598-021-87590-4Comparisons in temperature and photoperiodic-dependent diapause induction between domestic and wild mulberry silkwormsTakeshi Yokoyama0Shigeru Saito1Misato Shimoda2Masakazu Kobayashi3Yoko Takasu4Hideki Sezutsu5Yoshiomi Kato6Makoto Tominaga7Akira Mizoguchi8Kunihiro Shiomi9Department of United Graduate School of Agricultural Science, Tokyo University of Agriculture and TechnologyDivision of Cell Signaling, National Institute for Physiological Sciences, National Institutes of Natural SciencesGunma Sericultural Technology CenterFaculty of Textile Science and Technology, Shinshu UniversityNational Institute of Agrobiological Sciences (NIAS)National Institute of Agrobiological Sciences (NIAS)International Christian UniversityDivision of Cell Signaling, National Institute for Physiological Sciences, National Institutes of Natural SciencesDivision of Liberal Arts and Sciences, Aichi Gakuin UniversityFaculty of Textile Science and Technology, Shinshu UniversityAbstract The bivoltine strain of the domestic silkworm, Bombyx mori, has two generations per year. It shows a facultative diapause phenotype determined by environmental conditions, including photoperiod and temperature, and nutrient conditions during embryonic and larval development of the mother. However, it remains unclear how the environmental signals received during development are selectively utilized as cues to determine alternative diapause phenotypes. We performed a comparative analysis between the Kosetsu strain of B. mori and a Japanese population of the wild mulberry silkworm B. mandarina concerning the hierarchical molecular mechanisms in diapause induction. Our results showed that for the Kosetsu, temperature signals during the mother’s embryonic development predominantly affected diapause determination through the thermosensitive transient receptor potential ankyrin 1 (TRPA1) and diapause hormone (DH) signaling pathways. However, embryonic diapause in B. mandarina was photoperiod-dependent, although the DH signaling pathway and thermal sensitivity of TRPA1 were conserved within both species. Based on these findings, we hypothesize that TRPA1-activated signals are strongly linked to the signaling pathway participating in diapause induction in Kosetsu to selectively utilize the temperature information as the cue because temperature-dependent induction was replaced by photoperiodic induction in the TRPA1 knockout mutant.https://doi.org/10.1038/s41598-021-87590-4 |
| spellingShingle | Takeshi Yokoyama Shigeru Saito Misato Shimoda Masakazu Kobayashi Yoko Takasu Hideki Sezutsu Yoshiomi Kato Makoto Tominaga Akira Mizoguchi Kunihiro Shiomi Comparisons in temperature and photoperiodic-dependent diapause induction between domestic and wild mulberry silkworms Scientific Reports |
| title | Comparisons in temperature and photoperiodic-dependent diapause induction between domestic and wild mulberry silkworms |
| title_full | Comparisons in temperature and photoperiodic-dependent diapause induction between domestic and wild mulberry silkworms |
| title_fullStr | Comparisons in temperature and photoperiodic-dependent diapause induction between domestic and wild mulberry silkworms |
| title_full_unstemmed | Comparisons in temperature and photoperiodic-dependent diapause induction between domestic and wild mulberry silkworms |
| title_short | Comparisons in temperature and photoperiodic-dependent diapause induction between domestic and wild mulberry silkworms |
| title_sort | comparisons in temperature and photoperiodic dependent diapause induction between domestic and wild mulberry silkworms |
| url | https://doi.org/10.1038/s41598-021-87590-4 |
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