Transmembrane Protein LMxysn_<i>1693</i> of Serovar 4h <i>Listeria monocytogenes</i> Is Associated with Bile Salt Resistance and Intestinal Colonization
<i>Listeria monocytogenes</i> (Lm) is a ubiquitous foodborne pathogen comprising of 14 serotypes, of which serovar 4h isolates belonging to hybrid sub-lineage Ⅱ exhibit hypervirulent features. LMxysn_<i>1693</i> of serovar 4h Lm XYSN, a member of genomic island-7 (GI-7), is p...
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MDPI AG
2022-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/7/1263 |
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| author | Fanxin Jin Youwei Feng Chao Chen Hao Yao Renling Zhang Qin Zhang Fanzeng Meng Xiang Chen Xin’an Jiao Yuelan Yin |
| author_facet | Fanxin Jin Youwei Feng Chao Chen Hao Yao Renling Zhang Qin Zhang Fanzeng Meng Xiang Chen Xin’an Jiao Yuelan Yin |
| author_sort | Fanxin Jin |
| collection | DOAJ |
| description | <i>Listeria monocytogenes</i> (Lm) is a ubiquitous foodborne pathogen comprising of 14 serotypes, of which serovar 4h isolates belonging to hybrid sub-lineage Ⅱ exhibit hypervirulent features. LMxysn_<i>1693</i> of serovar 4h Lm XYSN, a member of genomic island-7 (GI-7), is predicted to a membrane protein with unknown function, which is conserved in serovar 4h <i>Listeria monocytogenes</i>. Under bile salts stress, Lm XYSN strain lacking LMxysn_<i>1693</i> (XYSN∆<i>1693</i>) exhibited a stationary phase growth defect as well as a reduction in biofilm formation and strikingly down-regulated bile-salts-resistant genes and virulent genes. Particularly, LMxysn_1693 protein plays a crucial role in Lm XYSN adhesion and invasion to intestinal epithelial cells, as well as colonization in the ileum of mice. Taken together, these findings indicate that the LMxysn_<i>1693</i> gene encodes a component of the putative ABC transporter system, synthetically interacts with genes involved in bile resistance, biofilm formation and virulence, and thus contributes to <i>Listeria monocytogenes</i> survival within and outside the host. |
| first_indexed | 2024-03-09T06:11:59Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T06:11:59Z |
| publishDate | 2022-06-01 |
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| series | Microorganisms |
| spelling | doaj.art-d61f554938e743ff979e6ab3b2551fe32023-12-03T11:58:26ZengMDPI AGMicroorganisms2076-26072022-06-01107126310.3390/microorganisms10071263Transmembrane Protein LMxysn_<i>1693</i> of Serovar 4h <i>Listeria monocytogenes</i> Is Associated with Bile Salt Resistance and Intestinal ColonizationFanxin Jin0Youwei Feng1Chao Chen2Hao Yao3Renling Zhang4Qin Zhang5Fanzeng Meng6Xiang Chen7Xin’an Jiao8Yuelan Yin9Jiangsu Key Laboratory of Zoonosis, Yangzhou University, 48 East Wenhui Road, Yangzhou 225009, ChinaJiangsu Key Laboratory of Zoonosis, Yangzhou University, 48 East Wenhui Road, Yangzhou 225009, ChinaJiangsu Key Laboratory of Zoonosis, Yangzhou University, 48 East Wenhui Road, Yangzhou 225009, ChinaJiangsu Key Laboratory of Zoonosis, Yangzhou University, 48 East Wenhui Road, Yangzhou 225009, ChinaJiangsu Key Laboratory of Zoonosis, Yangzhou University, 48 East Wenhui Road, Yangzhou 225009, ChinaJiangsu Key Laboratory of Zoonosis, Yangzhou University, 48 East Wenhui Road, Yangzhou 225009, ChinaJiangsu Key Laboratory of Zoonosis, Yangzhou University, 48 East Wenhui Road, Yangzhou 225009, ChinaJiangsu Key Laboratory of Zoonosis, Yangzhou University, 48 East Wenhui Road, Yangzhou 225009, ChinaJiangsu Key Laboratory of Zoonosis, Yangzhou University, 48 East Wenhui Road, Yangzhou 225009, ChinaJiangsu Key Laboratory of Zoonosis, Yangzhou University, 48 East Wenhui Road, Yangzhou 225009, China<i>Listeria monocytogenes</i> (Lm) is a ubiquitous foodborne pathogen comprising of 14 serotypes, of which serovar 4h isolates belonging to hybrid sub-lineage Ⅱ exhibit hypervirulent features. LMxysn_<i>1693</i> of serovar 4h Lm XYSN, a member of genomic island-7 (GI-7), is predicted to a membrane protein with unknown function, which is conserved in serovar 4h <i>Listeria monocytogenes</i>. Under bile salts stress, Lm XYSN strain lacking LMxysn_<i>1693</i> (XYSN∆<i>1693</i>) exhibited a stationary phase growth defect as well as a reduction in biofilm formation and strikingly down-regulated bile-salts-resistant genes and virulent genes. Particularly, LMxysn_1693 protein plays a crucial role in Lm XYSN adhesion and invasion to intestinal epithelial cells, as well as colonization in the ileum of mice. Taken together, these findings indicate that the LMxysn_<i>1693</i> gene encodes a component of the putative ABC transporter system, synthetically interacts with genes involved in bile resistance, biofilm formation and virulence, and thus contributes to <i>Listeria monocytogenes</i> survival within and outside the host.https://www.mdpi.com/2076-2607/10/7/1263<i>Listeria monocytogenes</i>bile saltvirulencebiofilmABC transportergenomic island |
| spellingShingle | Fanxin Jin Youwei Feng Chao Chen Hao Yao Renling Zhang Qin Zhang Fanzeng Meng Xiang Chen Xin’an Jiao Yuelan Yin Transmembrane Protein LMxysn_<i>1693</i> of Serovar 4h <i>Listeria monocytogenes</i> Is Associated with Bile Salt Resistance and Intestinal Colonization Microorganisms <i>Listeria monocytogenes</i> bile salt virulence biofilm ABC transporter genomic island |
| title | Transmembrane Protein LMxysn_<i>1693</i> of Serovar 4h <i>Listeria monocytogenes</i> Is Associated with Bile Salt Resistance and Intestinal Colonization |
| title_full | Transmembrane Protein LMxysn_<i>1693</i> of Serovar 4h <i>Listeria monocytogenes</i> Is Associated with Bile Salt Resistance and Intestinal Colonization |
| title_fullStr | Transmembrane Protein LMxysn_<i>1693</i> of Serovar 4h <i>Listeria monocytogenes</i> Is Associated with Bile Salt Resistance and Intestinal Colonization |
| title_full_unstemmed | Transmembrane Protein LMxysn_<i>1693</i> of Serovar 4h <i>Listeria monocytogenes</i> Is Associated with Bile Salt Resistance and Intestinal Colonization |
| title_short | Transmembrane Protein LMxysn_<i>1693</i> of Serovar 4h <i>Listeria monocytogenes</i> Is Associated with Bile Salt Resistance and Intestinal Colonization |
| title_sort | transmembrane protein lmxysn i 1693 i of serovar 4h i listeria monocytogenes i is associated with bile salt resistance and intestinal colonization |
| topic | <i>Listeria monocytogenes</i> bile salt virulence biofilm ABC transporter genomic island |
| url | https://www.mdpi.com/2076-2607/10/7/1263 |
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