Promotion effects and mechanisms of molybdenum disulfide on the propagation of antibiotic resistance genes in soil
The rapid development of nanotechnology has aroused considerable attentions toward understanding the effects of engineered nanomaterials (ENMs) on the propagation of antibiotic resistance. Molybdenum disulfide (MoS2) is an extensively used ENM and poses potential risks associated with environmental...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323004177 |
| _version_ | 1797840505367691264 |
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| author | Caixia Jin Jingxin Cao Kai Zhang Xingli Zhang Zhiguo Cao Wei Zou |
| author_facet | Caixia Jin Jingxin Cao Kai Zhang Xingli Zhang Zhiguo Cao Wei Zou |
| author_sort | Caixia Jin |
| collection | DOAJ |
| description | The rapid development of nanotechnology has aroused considerable attentions toward understanding the effects of engineered nanomaterials (ENMs) on the propagation of antibiotic resistance. Molybdenum disulfide (MoS2) is an extensively used ENM and poses potential risks associated with environmental exposure; nevertheless, the role of MoS2 toward antibiotic resistance genes (ARGs) transfer remains largely unknown. Herein, it was discovered that MoS2 nanosheets accelerated the horizontal transfer of RP4 plasmid across Escherichia coli in a dose-dependent manner (0.5–10 mg/L), with the maximum transfer frequency 2.07-fold higher than that of the control. Integration of physiological, transcriptomics, and metabolomics analyses demonstrated that SOS response in bacteria was activated by MoS2 due to the elevation of oxidative damage, accompanied by cell membrane permeabilization. MoS2 promoted bacterial adhesion and intercellular contact via stimulating the secretion of extracellular polysaccharides. The ATP levels were maximally increased by 305.7 % upon exposure to MoS2, and the expression of plasmid transfer genes was up-regulated, contributing to the accelerated plasmid conjugation and increased ARG abundance in soil. Our findings highlight the roles of emerging ENMs (e.g., MoS2) in ARGs dissemination, which is significant for the safe applications and risk management of ENMs under the development scenarios of nanotechnology. |
| first_indexed | 2024-04-09T16:16:26Z |
| format | Article |
| id | doaj.art-d620f3a7483f4d5980c00d297a1a7ba8 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-09T16:16:26Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-d620f3a7483f4d5980c00d297a1a7ba82023-04-24T04:37:44ZengElsevierEcotoxicology and Environmental Safety0147-65132023-05-01256114913Promotion effects and mechanisms of molybdenum disulfide on the propagation of antibiotic resistance genes in soilCaixia Jin0Jingxin Cao1Kai Zhang2Xingli Zhang3Zhiguo Cao4Wei Zou5School of Environment, Key Laboratory for Yellow River and Huai River Water Environment and Pollution Control, Ministry of Education, Henan Key Laboratory for Environmental Pollution Control, International Joint Laboratory on Key Techniques in Water Treatment, Henan Normal University, Xinxiang 453007, ChinaSchool of Environment, Key Laboratory for Yellow River and Huai River Water Environment and Pollution Control, Ministry of Education, Henan Key Laboratory for Environmental Pollution Control, International Joint Laboratory on Key Techniques in Water Treatment, Henan Normal University, Xinxiang 453007, ChinaSchool of Geographic Sciences, Henan Key Laboratory for Synergistic Prevention of Water and Soil Environmental Pollution, Xinyang Normal University, Xinyang 464000, ChinaSchool of Environment, Key Laboratory for Yellow River and Huai River Water Environment and Pollution Control, Ministry of Education, Henan Key Laboratory for Environmental Pollution Control, International Joint Laboratory on Key Techniques in Water Treatment, Henan Normal University, Xinxiang 453007, ChinaSchool of Environment, Key Laboratory for Yellow River and Huai River Water Environment and Pollution Control, Ministry of Education, Henan Key Laboratory for Environmental Pollution Control, International Joint Laboratory on Key Techniques in Water Treatment, Henan Normal University, Xinxiang 453007, ChinaSchool of Environment, Key Laboratory for Yellow River and Huai River Water Environment and Pollution Control, Ministry of Education, Henan Key Laboratory for Environmental Pollution Control, International Joint Laboratory on Key Techniques in Water Treatment, Henan Normal University, Xinxiang 453007, China; Corresponding author.The rapid development of nanotechnology has aroused considerable attentions toward understanding the effects of engineered nanomaterials (ENMs) on the propagation of antibiotic resistance. Molybdenum disulfide (MoS2) is an extensively used ENM and poses potential risks associated with environmental exposure; nevertheless, the role of MoS2 toward antibiotic resistance genes (ARGs) transfer remains largely unknown. Herein, it was discovered that MoS2 nanosheets accelerated the horizontal transfer of RP4 plasmid across Escherichia coli in a dose-dependent manner (0.5–10 mg/L), with the maximum transfer frequency 2.07-fold higher than that of the control. Integration of physiological, transcriptomics, and metabolomics analyses demonstrated that SOS response in bacteria was activated by MoS2 due to the elevation of oxidative damage, accompanied by cell membrane permeabilization. MoS2 promoted bacterial adhesion and intercellular contact via stimulating the secretion of extracellular polysaccharides. The ATP levels were maximally increased by 305.7 % upon exposure to MoS2, and the expression of plasmid transfer genes was up-regulated, contributing to the accelerated plasmid conjugation and increased ARG abundance in soil. Our findings highlight the roles of emerging ENMs (e.g., MoS2) in ARGs dissemination, which is significant for the safe applications and risk management of ENMs under the development scenarios of nanotechnology.http://www.sciencedirect.com/science/article/pii/S0147651323004177Antibiotic resistance geneEngineered nanomaterialsHorizontal transferOxidative stressSoil environment |
| spellingShingle | Caixia Jin Jingxin Cao Kai Zhang Xingli Zhang Zhiguo Cao Wei Zou Promotion effects and mechanisms of molybdenum disulfide on the propagation of antibiotic resistance genes in soil Ecotoxicology and Environmental Safety Antibiotic resistance gene Engineered nanomaterials Horizontal transfer Oxidative stress Soil environment |
| title | Promotion effects and mechanisms of molybdenum disulfide on the propagation of antibiotic resistance genes in soil |
| title_full | Promotion effects and mechanisms of molybdenum disulfide on the propagation of antibiotic resistance genes in soil |
| title_fullStr | Promotion effects and mechanisms of molybdenum disulfide on the propagation of antibiotic resistance genes in soil |
| title_full_unstemmed | Promotion effects and mechanisms of molybdenum disulfide on the propagation of antibiotic resistance genes in soil |
| title_short | Promotion effects and mechanisms of molybdenum disulfide on the propagation of antibiotic resistance genes in soil |
| title_sort | promotion effects and mechanisms of molybdenum disulfide on the propagation of antibiotic resistance genes in soil |
| topic | Antibiotic resistance gene Engineered nanomaterials Horizontal transfer Oxidative stress Soil environment |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323004177 |
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