Bacterial Detection and Recovery From Poultry Litter
The level of pathogens in poultry litter used for raising broiler chickens is critical to the overall health of a broiler chicken flock and food safety. Therefore, it is imperative that methods used for determining bacterial concentration in litter are accurate and reproducible across studies. In th...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.803150/full |
| _version_ | 1828147461395316736 |
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| author | Jodie R. Plumblee Lawrence Denice Cudnik Adelumola Oladeinde |
| author_facet | Jodie R. Plumblee Lawrence Denice Cudnik Adelumola Oladeinde |
| author_sort | Jodie R. Plumblee Lawrence |
| collection | DOAJ |
| description | The level of pathogens in poultry litter used for raising broiler chickens is critical to the overall health of a broiler chicken flock and food safety. Therefore, it is imperative that methods used for determining bacterial concentration in litter are accurate and reproducible across studies. In this perspective, we discuss the shortcomings associated with current methods used for bacterial quantification and detection from litter and assess the efficacy of one method for pathogen and commensal (Campylobacter, Salmonella, Escherichia coli, and Enterococcus spp.) recovery. The limit of quantitation and detection for this method differed between pathogens, and the recovery rate (∼138–208%) was higher for Salmonella, E. coli, and Enterococcus compared to Campylobacter (24%). Our results suggest that pathogen recovery from litter is highly variable and pathogen concentrations need to be reported in dry weight before comparisons can be made between studies. |
| first_indexed | 2024-04-11T21:01:25Z |
| format | Article |
| id | doaj.art-d62335b1809e4a89a86f044cd6e25bbd |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T21:01:25Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-d62335b1809e4a89a86f044cd6e25bbd2022-12-22T04:03:30ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-01-011210.3389/fmicb.2021.803150803150Bacterial Detection and Recovery From Poultry LitterJodie R. Plumblee LawrenceDenice CudnikAdelumola OladeindeThe level of pathogens in poultry litter used for raising broiler chickens is critical to the overall health of a broiler chicken flock and food safety. Therefore, it is imperative that methods used for determining bacterial concentration in litter are accurate and reproducible across studies. In this perspective, we discuss the shortcomings associated with current methods used for bacterial quantification and detection from litter and assess the efficacy of one method for pathogen and commensal (Campylobacter, Salmonella, Escherichia coli, and Enterococcus spp.) recovery. The limit of quantitation and detection for this method differed between pathogens, and the recovery rate (∼138–208%) was higher for Salmonella, E. coli, and Enterococcus compared to Campylobacter (24%). Our results suggest that pathogen recovery from litter is highly variable and pathogen concentrations need to be reported in dry weight before comparisons can be made between studies.https://www.frontiersin.org/articles/10.3389/fmicb.2021.803150/fullpoultry litterfood-borne pathogenslimit of detectionlimit of quantitationfood safetymethod validation |
| spellingShingle | Jodie R. Plumblee Lawrence Denice Cudnik Adelumola Oladeinde Bacterial Detection and Recovery From Poultry Litter Frontiers in Microbiology poultry litter food-borne pathogens limit of detection limit of quantitation food safety method validation |
| title | Bacterial Detection and Recovery From Poultry Litter |
| title_full | Bacterial Detection and Recovery From Poultry Litter |
| title_fullStr | Bacterial Detection and Recovery From Poultry Litter |
| title_full_unstemmed | Bacterial Detection and Recovery From Poultry Litter |
| title_short | Bacterial Detection and Recovery From Poultry Litter |
| title_sort | bacterial detection and recovery from poultry litter |
| topic | poultry litter food-borne pathogens limit of detection limit of quantitation food safety method validation |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.803150/full |
| work_keys_str_mv | AT jodierplumbleelawrence bacterialdetectionandrecoveryfrompoultrylitter AT denicecudnik bacterialdetectionandrecoveryfrompoultrylitter AT adelumolaoladeinde bacterialdetectionandrecoveryfrompoultrylitter |