Tracking Carbapenem-Producing Klebsiella pneumoniae Outbreak in an Intensive Care Unit by Whole Genome Sequencing
The presence of carbapenem-producing Klebsiella pneumoniae (CP-Kp) is a serious threat to the control of nosocomial infections. Plasmid-mediated horizontal transfer of the resistance gene makes it difficult to control hospital-acquired CP- Kp infections. Nine CP- Kp strains were isolated during an o...
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| Language: | English |
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Frontiers Media S.A.
2019-08-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fcimb.2019.00281/full |
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| author | Chen Chen Yi Zhang Sheng-Lei Yu Yang Zhou Si-Yu Yang Jia-Lin Jin Shu Chen Peng Cui Jing Wu Ning Jiang Wen-Hong Zhang Wen-Hong Zhang |
| author_facet | Chen Chen Yi Zhang Sheng-Lei Yu Yang Zhou Si-Yu Yang Jia-Lin Jin Shu Chen Peng Cui Jing Wu Ning Jiang Wen-Hong Zhang Wen-Hong Zhang |
| author_sort | Chen Chen |
| collection | DOAJ |
| description | The presence of carbapenem-producing Klebsiella pneumoniae (CP-Kp) is a serious threat to the control of nosocomial infections. Plasmid-mediated horizontal transfer of the resistance gene makes it difficult to control hospital-acquired CP- Kp infections. Nine CP- Kp strains were isolated during an outbreak in the intensive care unit of Shanghai Huashan hospital in east China. We conducted a retrospective study to identify the origin and route of transmission of this CP-Kp outbreak. Whole-genome sequencing (WGS) analysis was performed on 9 clinical isolates obtained from 8 patients, and the results were compared to clinical and epidemiological records. All isolates were ST11 CP-Kp. Single-nucleotide polymorphisms and the presence and structure of plasmids indicated that this CP-Kp outbreak had different origins. These 9 isolates were partitioned into two clades according to genetic distance. Four plasmids, CP002474.1, CP006799.1, CP018455.1, and CP025459.1, were detected among the 9 isolates. The plasmid phylogeny and antibiotic resistance (AR) gene profile results were consistent with the sequencing results. We found that two clades of CP-Kp were responsible for this nosocomial outbreak and demonstrated the transmission route from two index patients. Plasmid carriage and phylogeny are a useful tool for identifying clades involved in disease transmission. |
| first_indexed | 2024-04-14T07:38:53Z |
| format | Article |
| id | doaj.art-d623ddf0468a4d738514a32a8b20bace |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-14T07:38:53Z |
| publishDate | 2019-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-d623ddf0468a4d738514a32a8b20bace2022-12-22T02:05:36ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882019-08-01910.3389/fcimb.2019.00281472219Tracking Carbapenem-Producing Klebsiella pneumoniae Outbreak in an Intensive Care Unit by Whole Genome SequencingChen Chen0Yi Zhang1Sheng-Lei Yu2Yang Zhou3Si-Yu Yang4Jia-Lin Jin5Shu Chen6Peng Cui7Jing Wu8Ning Jiang9Wen-Hong Zhang10Wen-Hong Zhang11Department of Infectious Disease, Huashan Hospital of Fudan University, Shanghai, ChinaDepartment of Infectious Disease, Huashan Hospital of Fudan University, Shanghai, ChinaDepartment of Infectious Disease, Huashan Hospital of Fudan University, Shanghai, ChinaDepartment of Infectious Disease, Huashan Hospital of Fudan University, Shanghai, ChinaDepartment of Infectious Disease, Huashan Hospital of Fudan University, Shanghai, ChinaDepartment of Infectious Disease, Huashan Hospital of Fudan University, Shanghai, ChinaDepartment of Infectious Disease, Huashan Hospital of Fudan University, Shanghai, ChinaDepartment of Infectious Disease, Huashan Hospital of Fudan University, Shanghai, ChinaDepartment of Infectious Disease, Huashan Hospital of Fudan University, Shanghai, ChinaState Key Laboratory of Genetic Engineering and Institute of Biostatistics, School of Life Sciences, Fudan University, Shanghai, ChinaDepartment of Infectious Disease, Huashan Hospital of Fudan University, Shanghai, ChinaNational Clinical Research Center for Aging and Medicine, Huashan Hospital of Fudan University, Shanghai, ChinaThe presence of carbapenem-producing Klebsiella pneumoniae (CP-Kp) is a serious threat to the control of nosocomial infections. Plasmid-mediated horizontal transfer of the resistance gene makes it difficult to control hospital-acquired CP- Kp infections. Nine CP- Kp strains were isolated during an outbreak in the intensive care unit of Shanghai Huashan hospital in east China. We conducted a retrospective study to identify the origin and route of transmission of this CP-Kp outbreak. Whole-genome sequencing (WGS) analysis was performed on 9 clinical isolates obtained from 8 patients, and the results were compared to clinical and epidemiological records. All isolates were ST11 CP-Kp. Single-nucleotide polymorphisms and the presence and structure of plasmids indicated that this CP-Kp outbreak had different origins. These 9 isolates were partitioned into two clades according to genetic distance. Four plasmids, CP002474.1, CP006799.1, CP018455.1, and CP025459.1, were detected among the 9 isolates. The plasmid phylogeny and antibiotic resistance (AR) gene profile results were consistent with the sequencing results. We found that two clades of CP-Kp were responsible for this nosocomial outbreak and demonstrated the transmission route from two index patients. Plasmid carriage and phylogeny are a useful tool for identifying clades involved in disease transmission.https://www.frontiersin.org/article/10.3389/fcimb.2019.00281/fullcarbapenemase-producingKlebsiella pneumoniaewhole genome sequencingoutbreaktransmission |
| spellingShingle | Chen Chen Yi Zhang Sheng-Lei Yu Yang Zhou Si-Yu Yang Jia-Lin Jin Shu Chen Peng Cui Jing Wu Ning Jiang Wen-Hong Zhang Wen-Hong Zhang Tracking Carbapenem-Producing Klebsiella pneumoniae Outbreak in an Intensive Care Unit by Whole Genome Sequencing Frontiers in Cellular and Infection Microbiology carbapenemase-producing Klebsiella pneumoniae whole genome sequencing outbreak transmission |
| title | Tracking Carbapenem-Producing Klebsiella pneumoniae Outbreak in an Intensive Care Unit by Whole Genome Sequencing |
| title_full | Tracking Carbapenem-Producing Klebsiella pneumoniae Outbreak in an Intensive Care Unit by Whole Genome Sequencing |
| title_fullStr | Tracking Carbapenem-Producing Klebsiella pneumoniae Outbreak in an Intensive Care Unit by Whole Genome Sequencing |
| title_full_unstemmed | Tracking Carbapenem-Producing Klebsiella pneumoniae Outbreak in an Intensive Care Unit by Whole Genome Sequencing |
| title_short | Tracking Carbapenem-Producing Klebsiella pneumoniae Outbreak in an Intensive Care Unit by Whole Genome Sequencing |
| title_sort | tracking carbapenem producing klebsiella pneumoniae outbreak in an intensive care unit by whole genome sequencing |
| topic | carbapenemase-producing Klebsiella pneumoniae whole genome sequencing outbreak transmission |
| url | https://www.frontiersin.org/article/10.3389/fcimb.2019.00281/full |
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