In vitro antibacterial activities of silver nanoparticles synthesised using the seed extracts of three varieties of Phoenix dactylifera
Abstract Green synthesis of silver nanoparticles (AgNPs) is an ecofriendly, cost-effective and promising approach for discovery of novel therapeutics. The aim of the current work was to biogenic synthesize, characterize AgNPs using seed extracts of three economically important varieties of date palm...
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| Format: | Article |
| Language: | English |
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Instituto Internacional de Ecologia
2021-07-01
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| Series: | Brazilian Journal of Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842022000100257&tlng=en |
| _version_ | 1830380686401339392 |
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| author | F. M. Aldayel M. S. Alsobeg A. Khalifa |
| author_facet | F. M. Aldayel M. S. Alsobeg A. Khalifa |
| author_sort | F. M. Aldayel |
| collection | DOAJ |
| description | Abstract Green synthesis of silver nanoparticles (AgNPs) is an ecofriendly, cost-effective and promising approach for discovery of novel therapeutics. The aim of the current work was to biogenic synthesize, characterize AgNPs using seed extracts of three economically important varieties of date palm (Iklas, Irziz and Shishi), and assess their anti-pathogenic bacterial activities. AgNPs were synthesised then characterised using electron microscopy and Fourier transform infrared analyses. The bactericidal activities of AgNPs against five different bacterial pathogens, Bacillus subtilis, Escherichia coli, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Streptococcus pneumoniae, were determined in vitro. In particular, changes in membrane integrity of virulent bacterial strains in response to AgNPs were investigated. Results of lactate dehydrogenase, alkaline phosphatase activity assays, and measurement of membrane potential revealed that the cytotoxic effects of the AgNPs were mainly centred on the plasma membrane of bacterial cells, leading to loss of its integrity and eventually cell death. In conclusion, green synthesis of AgNPs is an efficient, cost-effective and promising strategy to combat virulent antibiotic-resistant strains. |
| first_indexed | 2024-12-20T09:35:33Z |
| format | Article |
| id | doaj.art-d62ac44984884e62b2f0cbe5f71a17d4 |
| institution | Directory Open Access Journal |
| issn | 1678-4375 |
| language | English |
| last_indexed | 2024-12-20T09:35:33Z |
| publishDate | 2021-07-01 |
| publisher | Instituto Internacional de Ecologia |
| record_format | Article |
| series | Brazilian Journal of Biology |
| spelling | doaj.art-d62ac44984884e62b2f0cbe5f71a17d42022-12-21T19:45:01ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-43752021-07-018210.1590/1519-6984.242301In vitro antibacterial activities of silver nanoparticles synthesised using the seed extracts of three varieties of Phoenix dactyliferaF. M. Aldayelhttps://orcid.org/0000-0003-2732-6230M. S. Alsobeghttps://orcid.org/0000-0002-4981-8415A. Khalifahttps://orcid.org/0000-0003-4272-929XAbstract Green synthesis of silver nanoparticles (AgNPs) is an ecofriendly, cost-effective and promising approach for discovery of novel therapeutics. The aim of the current work was to biogenic synthesize, characterize AgNPs using seed extracts of three economically important varieties of date palm (Iklas, Irziz and Shishi), and assess their anti-pathogenic bacterial activities. AgNPs were synthesised then characterised using electron microscopy and Fourier transform infrared analyses. The bactericidal activities of AgNPs against five different bacterial pathogens, Bacillus subtilis, Escherichia coli, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Streptococcus pneumoniae, were determined in vitro. In particular, changes in membrane integrity of virulent bacterial strains in response to AgNPs were investigated. Results of lactate dehydrogenase, alkaline phosphatase activity assays, and measurement of membrane potential revealed that the cytotoxic effects of the AgNPs were mainly centred on the plasma membrane of bacterial cells, leading to loss of its integrity and eventually cell death. In conclusion, green synthesis of AgNPs is an efficient, cost-effective and promising strategy to combat virulent antibiotic-resistant strains.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842022000100257&tlng=enIklasIrziz and ShishiAgNPsFTIRantimicrobial activity, |
| spellingShingle | F. M. Aldayel M. S. Alsobeg A. Khalifa In vitro antibacterial activities of silver nanoparticles synthesised using the seed extracts of three varieties of Phoenix dactylifera Brazilian Journal of Biology Iklas Irziz and Shishi AgNPs FTIR antimicrobial activity, |
| title | In vitro antibacterial activities of silver nanoparticles synthesised using the seed extracts of three varieties of Phoenix dactylifera |
| title_full | In vitro antibacterial activities of silver nanoparticles synthesised using the seed extracts of three varieties of Phoenix dactylifera |
| title_fullStr | In vitro antibacterial activities of silver nanoparticles synthesised using the seed extracts of three varieties of Phoenix dactylifera |
| title_full_unstemmed | In vitro antibacterial activities of silver nanoparticles synthesised using the seed extracts of three varieties of Phoenix dactylifera |
| title_short | In vitro antibacterial activities of silver nanoparticles synthesised using the seed extracts of three varieties of Phoenix dactylifera |
| title_sort | in vitro antibacterial activities of silver nanoparticles synthesised using the seed extracts of three varieties of phoenix dactylifera |
| topic | Iklas Irziz and Shishi AgNPs FTIR antimicrobial activity, |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842022000100257&tlng=en |
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