Dietary Cholesterol Differentially Regulates the Muscle Lipidomics of Farmed Turbot and Tiger Puffer
Exogenous cholesterol has been supplemented into aqua-feeds due to the reduced proportions of fishmeal and fish oil. This study aimed to investigate the effects of dietary cholesterol supplementation on the muscle lipidomics of two marine fish species, turbot and tiger puffer. A 70-day feeding trial...
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MDPI AG
2023-05-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/10/1632 |
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| author | Xiaoxue Meng Qingzhu Bi Qiang Ma Yuliang Wei Yanlu Li Mengqing Liang Houguo Xu |
| author_facet | Xiaoxue Meng Qingzhu Bi Qiang Ma Yuliang Wei Yanlu Li Mengqing Liang Houguo Xu |
| author_sort | Xiaoxue Meng |
| collection | DOAJ |
| description | Exogenous cholesterol has been supplemented into aqua-feeds due to the reduced proportions of fishmeal and fish oil. This study aimed to investigate the effects of dietary cholesterol supplementation on the muscle lipidomics of two marine fish species, turbot and tiger puffer. A 70-day feeding trial was conducted, where two low-fishmeal diets supplemented with 0 or 1% cholesterol were used. The lipidomic analysis with targeted tandem mass spectrometry showed that, in turbot, a total of 49 individual lipids exhibited significant differences in their abundance in response to dietary cholesterol, whereas the number was 30 for tiger puffer. Dietary cholesterol up-regulated the abundance of cholesterol and cholesterol ester in both species. In turbot, the dietary cholesterol also increased the abundance of triacylglycerol and acylcarnitine, whereas in tiger puffer, it primarily regulated the abundance of phospholipids and BMP. This was the first time the responses of marine fish muscle lipidomics to dietary cholesterol supplementation have been investigated. |
| first_indexed | 2024-03-11T04:01:01Z |
| format | Article |
| id | doaj.art-d62d02776d6b4d4bb4565271993da30b |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T04:01:01Z |
| publishDate | 2023-05-01 |
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| record_format | Article |
| series | Animals |
| spelling | doaj.art-d62d02776d6b4d4bb4565271993da30b2023-11-18T00:09:56ZengMDPI AGAnimals2076-26152023-05-011310163210.3390/ani13101632Dietary Cholesterol Differentially Regulates the Muscle Lipidomics of Farmed Turbot and Tiger PufferXiaoxue Meng0Qingzhu Bi1Qiang Ma2Yuliang Wei3Yanlu Li4Mengqing Liang5Houguo Xu6Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, ChinaExogenous cholesterol has been supplemented into aqua-feeds due to the reduced proportions of fishmeal and fish oil. This study aimed to investigate the effects of dietary cholesterol supplementation on the muscle lipidomics of two marine fish species, turbot and tiger puffer. A 70-day feeding trial was conducted, where two low-fishmeal diets supplemented with 0 or 1% cholesterol were used. The lipidomic analysis with targeted tandem mass spectrometry showed that, in turbot, a total of 49 individual lipids exhibited significant differences in their abundance in response to dietary cholesterol, whereas the number was 30 for tiger puffer. Dietary cholesterol up-regulated the abundance of cholesterol and cholesterol ester in both species. In turbot, the dietary cholesterol also increased the abundance of triacylglycerol and acylcarnitine, whereas in tiger puffer, it primarily regulated the abundance of phospholipids and BMP. This was the first time the responses of marine fish muscle lipidomics to dietary cholesterol supplementation have been investigated.https://www.mdpi.com/2076-2615/13/10/1632flatfishpufferfishcholesterolmusclelipidomics |
| spellingShingle | Xiaoxue Meng Qingzhu Bi Qiang Ma Yuliang Wei Yanlu Li Mengqing Liang Houguo Xu Dietary Cholesterol Differentially Regulates the Muscle Lipidomics of Farmed Turbot and Tiger Puffer Animals flatfish pufferfish cholesterol muscle lipidomics |
| title | Dietary Cholesterol Differentially Regulates the Muscle Lipidomics of Farmed Turbot and Tiger Puffer |
| title_full | Dietary Cholesterol Differentially Regulates the Muscle Lipidomics of Farmed Turbot and Tiger Puffer |
| title_fullStr | Dietary Cholesterol Differentially Regulates the Muscle Lipidomics of Farmed Turbot and Tiger Puffer |
| title_full_unstemmed | Dietary Cholesterol Differentially Regulates the Muscle Lipidomics of Farmed Turbot and Tiger Puffer |
| title_short | Dietary Cholesterol Differentially Regulates the Muscle Lipidomics of Farmed Turbot and Tiger Puffer |
| title_sort | dietary cholesterol differentially regulates the muscle lipidomics of farmed turbot and tiger puffer |
| topic | flatfish pufferfish cholesterol muscle lipidomics |
| url | https://www.mdpi.com/2076-2615/13/10/1632 |
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