Susceptibility of larvae and pupae of T. nigritus to entomopathogenic nematode Heterorhabditis indica Poinar

In order to know the susceptibility of larvae and pupae of T.nigritusto entomopathogenic nematode H. indica , the present study is carried out to establish different concentrations of infective juveniles per each 0.25 kg soil (JI.soil-1) and a control treatment to which sterile water was applied....

Full description

Bibliographic Details
Main Authors: María del Carmen Castellón Valdés, Julián González Rodríguez, Edilberto Pozo Velásquez
Format: Article
Language:Spanish
Published: Universidad Central "Marta Abreu" de Las Villas 2014-07-01
Series:Centro Agrícola
Subjects:
Online Access:http://cagricola.uclv.edu.cu/index.php/es/volumen-41-2014/numero-3-2014/92-susceptibilidad-de-larvas-y-pupas-de-typophorus-nigritus-f-al-nematodo-entomopatogeno-heterorhabditis-indica-poinar
_version_ 1818961557524054016
author María del Carmen Castellón Valdés
Julián González Rodríguez
Edilberto Pozo Velásquez
author_facet María del Carmen Castellón Valdés
Julián González Rodríguez
Edilberto Pozo Velásquez
author_sort María del Carmen Castellón Valdés
collection DOAJ
description In order to know the susceptibility of larvae and pupae of T.nigritusto entomopathogenic nematode H. indica , the present study is carried out to establish different concentrations of infective juveniles per each 0.25 kg soil (JI.soil-1) and a control treatment to which sterile water was applied. Observations were made 24 hours after inoculating entomopathogenic nematodes and up to 96 hours, and larvae and pupae mortality was assessed. After ten days, individuals with mortality signs were transferred to Whife traps for removal of JI from insect corpses. This experiment was repeated with the use of JI emerged and harvested to check their effectiveness on T. nigritus . Larvae and pupae of T. nigritus showed susceptibility to entomopathogenic nematode H. indica in laboratory conditions. The mortality percentage for both development stages increased as the concentration of JI. soil-1 increased. Ten days after inoculation, nematodes (JI) emerged from the interior of the bodies, and were observed on their surfaces. At concentrations above 1 200 JI, 100 % mortality was achieved
first_indexed 2024-12-20T12:15:19Z
format Article
id doaj.art-d62fae20268b49b88624d56453272a90
institution Directory Open Access Journal
issn 0253-5785
2072-2001
language Spanish
last_indexed 2024-12-20T12:15:19Z
publishDate 2014-07-01
publisher Universidad Central "Marta Abreu" de Las Villas
record_format Article
series Centro Agrícola
spelling doaj.art-d62fae20268b49b88624d56453272a902022-12-21T19:41:09ZspaUniversidad Central "Marta Abreu" de Las VillasCentro Agrícola0253-57852072-20012014-07-014132328Susceptibility of larvae and pupae of T. nigritus to entomopathogenic nematode Heterorhabditis indica PoinarMaría del Carmen Castellón Valdés0 Julián González Rodríguez1Edilberto Pozo Velásquez2Instituto de Investigaciones de Viandas Tropicales (INIVIT). Apdo #6. Santo Domingo, Villa Clara, CubaInstituto de Investigaciones de Viandas Tropicales (INIVIT). Apdo #6. Santo Domingo, Villa Clara, CubaUniversidad Central “Marta Abreu” de Las Villas. Carretera a Camajuaní km 6½. Santa Clara, Villa Clara, CubaIn order to know the susceptibility of larvae and pupae of T.nigritusto entomopathogenic nematode H. indica , the present study is carried out to establish different concentrations of infective juveniles per each 0.25 kg soil (JI.soil-1) and a control treatment to which sterile water was applied. Observations were made 24 hours after inoculating entomopathogenic nematodes and up to 96 hours, and larvae and pupae mortality was assessed. After ten days, individuals with mortality signs were transferred to Whife traps for removal of JI from insect corpses. This experiment was repeated with the use of JI emerged and harvested to check their effectiveness on T. nigritus . Larvae and pupae of T. nigritus showed susceptibility to entomopathogenic nematode H. indica in laboratory conditions. The mortality percentage for both development stages increased as the concentration of JI. soil-1 increased. Ten days after inoculation, nematodes (JI) emerged from the interior of the bodies, and were observed on their surfaces. At concentrations above 1 200 JI, 100 % mortality was achievedhttp://cagricola.uclv.edu.cu/index.php/es/volumen-41-2014/numero-3-2014/92-susceptibilidad-de-larvas-y-pupas-de-typophorus-nigritus-f-al-nematodo-entomopatogeno-heterorhabditis-indica-poinarTypophorus nigritusHeterorhabditis indica
spellingShingle María del Carmen Castellón Valdés
Julián González Rodríguez
Edilberto Pozo Velásquez
Susceptibility of larvae and pupae of T. nigritus to entomopathogenic nematode Heterorhabditis indica Poinar
Centro Agrícola
Typophorus nigritus
Heterorhabditis indica
title Susceptibility of larvae and pupae of T. nigritus to entomopathogenic nematode Heterorhabditis indica Poinar
title_full Susceptibility of larvae and pupae of T. nigritus to entomopathogenic nematode Heterorhabditis indica Poinar
title_fullStr Susceptibility of larvae and pupae of T. nigritus to entomopathogenic nematode Heterorhabditis indica Poinar
title_full_unstemmed Susceptibility of larvae and pupae of T. nigritus to entomopathogenic nematode Heterorhabditis indica Poinar
title_short Susceptibility of larvae and pupae of T. nigritus to entomopathogenic nematode Heterorhabditis indica Poinar
title_sort susceptibility of larvae and pupae of t nigritus to entomopathogenic nematode heterorhabditis indica poinar
topic Typophorus nigritus
Heterorhabditis indica
url http://cagricola.uclv.edu.cu/index.php/es/volumen-41-2014/numero-3-2014/92-susceptibilidad-de-larvas-y-pupas-de-typophorus-nigritus-f-al-nematodo-entomopatogeno-heterorhabditis-indica-poinar
work_keys_str_mv AT mariadelcarmencastellonvaldes susceptibilityoflarvaeandpupaeoftnigritustoentomopathogenicnematodeheterorhabditisindicapoinar
AT juliangonzalezrodriguez susceptibilityoflarvaeandpupaeoftnigritustoentomopathogenicnematodeheterorhabditisindicapoinar
AT edilbertopozovelasquez susceptibilityoflarvaeandpupaeoftnigritustoentomopathogenicnematodeheterorhabditisindicapoinar