Evaluation of Green Super Rice Lines for Agronomic and Physiological Traits under Salinity Stress
Rice (<i>Oryza sativa</i>) is an important staple food crop worldwide, especially in east and southeast Asia. About one-third of rice cultivated area is under saline soil, either natural saline soils or irrigation with brackish water. Salinity stress is among the devastating abiotic stre...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/11/1461 |
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| author | Muhammad Ammar Amanat Muhammad Kashif Naeem Hussah I. M. Algwaiz Muhammad Uzair Kotb A. Attia Muneera D. F. AlKathani Imdad Ulah Zaid Syed Adeel Zafar Safeena Inam Sajid Fiaz Muhammad Hamza Arif Daniyal Ahmad Nageen Zahra Bilal Saleem Muhammad Ramzan Khan |
| author_facet | Muhammad Ammar Amanat Muhammad Kashif Naeem Hussah I. M. Algwaiz Muhammad Uzair Kotb A. Attia Muneera D. F. AlKathani Imdad Ulah Zaid Syed Adeel Zafar Safeena Inam Sajid Fiaz Muhammad Hamza Arif Daniyal Ahmad Nageen Zahra Bilal Saleem Muhammad Ramzan Khan |
| author_sort | Muhammad Ammar Amanat |
| collection | DOAJ |
| description | Rice (<i>Oryza sativa</i>) is an important staple food crop worldwide, especially in east and southeast Asia. About one-third of rice cultivated area is under saline soil, either natural saline soils or irrigation with brackish water. Salinity stress is among the devastating abiotic stresses that not only affect rice growth and crop productivity but also limit its cultivation area globally. Plants adopt multiple tolerance mechanisms at the morphological, physiological, and biochemical levels to tackle salinity stress. To identify these tolerance mechanisms, this study was carried out under both a controlled glass house as well as natural saline field conditions using 22 green super rice (GSR) lines along with two local varieties (“IRRI 6 and Kissan Basmati”). Several morpho-physiological and biochemical parameters along with stress-responsive genes were used as evaluation criteria under normal and salinity stress conditions. Correlation and Principal Component Analysis (PCA) suggested that shoot-related parameters and the salt susceptible index (SSI) can be used for the identification of salt-tolerant genotypes. Based on Agglomerative Hierarchical Cluster (AHC) analysis, two saline-tolerant (“S19 and S20”) and saline-susceptible (“S3 and S24”) lines were selected for further molecular evaluation. Quantitative RT-PCR was performed, and results showed that expression of 1-5-phosphoribosyl -5-5-phosphoribosyl amino methylidene amino imidazole-4-carboxamide isomerase, DNA repair protein recA, and peptide transporter PTR2 related genes were upregulated in salt-tolerant genotypes, suggesting their potential role in salinity tolerance. However, additional validation using reverse genetics approaches will further confirm their specific role in salt tolerance. Identified saline-tolerant lines in this study will be useful genetic resources for future salinity breeding programs. |
| first_indexed | 2024-03-10T00:59:05Z |
| format | Article |
| id | doaj.art-d633e584edd442cbb2e44f259e67b69f |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T00:59:05Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-d633e584edd442cbb2e44f259e67b69f2023-11-23T14:39:02ZengMDPI AGPlants2223-77472022-05-011111146110.3390/plants11111461Evaluation of Green Super Rice Lines for Agronomic and Physiological Traits under Salinity StressMuhammad Ammar Amanat0Muhammad Kashif Naeem1Hussah I. M. Algwaiz2Muhammad Uzair3Kotb A. Attia4Muneera D. F. AlKathani5Imdad Ulah Zaid6Syed Adeel Zafar7Safeena Inam8Sajid Fiaz9Muhammad Hamza Arif10Daniyal Ahmad11Nageen Zahra12Bilal Saleem13Muhammad Ramzan Khan14National Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre (NARC), Park Road, Islamabad 45500, PakistanNational Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre (NARC), Park Road, Islamabad 45500, PakistanDepartment of Biology, College of Science, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi ArabiaNational Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre (NARC), Park Road, Islamabad 45500, PakistanCenter of Excellence in Biotechnology Research, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Biology, College of Science, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi ArabiaNational Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre (NARC), Park Road, Islamabad 45500, PakistanNational Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre (NARC), Park Road, Islamabad 45500, PakistanNational Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre (NARC), Park Road, Islamabad 45500, PakistanDepartment of Plant Breeding and Genetics, The University of Haripur, Haripur 22620, PakistanNational Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre (NARC), Park Road, Islamabad 45500, PakistanNational Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre (NARC), Park Road, Islamabad 45500, PakistanNational Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre (NARC), Park Road, Islamabad 45500, PakistanNational Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre (NARC), Park Road, Islamabad 45500, PakistanNational Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre (NARC), Park Road, Islamabad 45500, PakistanRice (<i>Oryza sativa</i>) is an important staple food crop worldwide, especially in east and southeast Asia. About one-third of rice cultivated area is under saline soil, either natural saline soils or irrigation with brackish water. Salinity stress is among the devastating abiotic stresses that not only affect rice growth and crop productivity but also limit its cultivation area globally. Plants adopt multiple tolerance mechanisms at the morphological, physiological, and biochemical levels to tackle salinity stress. To identify these tolerance mechanisms, this study was carried out under both a controlled glass house as well as natural saline field conditions using 22 green super rice (GSR) lines along with two local varieties (“IRRI 6 and Kissan Basmati”). Several morpho-physiological and biochemical parameters along with stress-responsive genes were used as evaluation criteria under normal and salinity stress conditions. Correlation and Principal Component Analysis (PCA) suggested that shoot-related parameters and the salt susceptible index (SSI) can be used for the identification of salt-tolerant genotypes. Based on Agglomerative Hierarchical Cluster (AHC) analysis, two saline-tolerant (“S19 and S20”) and saline-susceptible (“S3 and S24”) lines were selected for further molecular evaluation. Quantitative RT-PCR was performed, and results showed that expression of 1-5-phosphoribosyl -5-5-phosphoribosyl amino methylidene amino imidazole-4-carboxamide isomerase, DNA repair protein recA, and peptide transporter PTR2 related genes were upregulated in salt-tolerant genotypes, suggesting their potential role in salinity tolerance. However, additional validation using reverse genetics approaches will further confirm their specific role in salt tolerance. Identified saline-tolerant lines in this study will be useful genetic resources for future salinity breeding programs.https://www.mdpi.com/2223-7747/11/11/1461green super ricesalinity stresssalinity stressfield evaluationgene expression |
| spellingShingle | Muhammad Ammar Amanat Muhammad Kashif Naeem Hussah I. M. Algwaiz Muhammad Uzair Kotb A. Attia Muneera D. F. AlKathani Imdad Ulah Zaid Syed Adeel Zafar Safeena Inam Sajid Fiaz Muhammad Hamza Arif Daniyal Ahmad Nageen Zahra Bilal Saleem Muhammad Ramzan Khan Evaluation of Green Super Rice Lines for Agronomic and Physiological Traits under Salinity Stress Plants green super rice salinity stress salinity stress field evaluation gene expression |
| title | Evaluation of Green Super Rice Lines for Agronomic and Physiological Traits under Salinity Stress |
| title_full | Evaluation of Green Super Rice Lines for Agronomic and Physiological Traits under Salinity Stress |
| title_fullStr | Evaluation of Green Super Rice Lines for Agronomic and Physiological Traits under Salinity Stress |
| title_full_unstemmed | Evaluation of Green Super Rice Lines for Agronomic and Physiological Traits under Salinity Stress |
| title_short | Evaluation of Green Super Rice Lines for Agronomic and Physiological Traits under Salinity Stress |
| title_sort | evaluation of green super rice lines for agronomic and physiological traits under salinity stress |
| topic | green super rice salinity stress salinity stress field evaluation gene expression |
| url | https://www.mdpi.com/2223-7747/11/11/1461 |
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