Morphological and molecular characterization of potato cyst nematode populations from The Nilgiris
Quarantine species of potato cyst nematodes (PCN), Globodera rostochiensis and G. pallida, were reported from Nilgiris during 1961. The studies were carried out at ICAR-CPRS, Muthorai, Ooty and ICAR-CPRI, Shimla during 2015–17. To investigate the distribution of PCN, soil samples were collected fro...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2020-03-01
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| Series: | The Indian Journal of Agricultural Sciences |
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| Online Access: | https://epubs.icar.org.in/index.php/IJAgS/article/view/99000 |
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| author | AARTI BAIRWA VENKATASALAM E P JEEVALATHA A PRIYANK H M TANUJA BUCKSETH JENIFER A SANJEEV SHARMA R K SINGH S K CHAKRABARTI |
| author_facet | AARTI BAIRWA VENKATASALAM E P JEEVALATHA A PRIYANK H M TANUJA BUCKSETH JENIFER A SANJEEV SHARMA R K SINGH S K CHAKRABARTI |
| author_sort | AARTI BAIRWA |
| collection | DOAJ |
| description |
Quarantine species of potato cyst nematodes (PCN), Globodera rostochiensis and G. pallida, were reported from Nilgiris during 1961. The studies were carried out at ICAR-CPRS, Muthorai, Ooty and ICAR-CPRI, Shimla during 2015–17. To investigate the distribution of PCN, soil samples were collected from potato growing areas of Nilgiris and were identified based on morphological criteria and ITS-1 region. Molecular characterization using ITS-1 region specific primers revealed the presence of pure population of G. rostochiensis in 50% of the samples, G. pallida in 10.7% of the samples, mixed population in 28.6% of the samples and absence of both the species in 10.7% of the samples. The phylogenetic analysis inferred by the sequence of the ITS-1 region confirmed 92–100% genetic similarities in Globodera spp. Seventeen isolates of G. rostochiensis showed 92–99% genetic similarity and rest four 92–100% similarities. Whereas, genetic similarity among the ten isolates of G. pallida was 96.1–99.4%. In the morphometric characters J2s of G. rostochiensis exhibited shorter body length (459.8 μm) than G. pallida (493.7 μm). G. rostochiensis and G. pallida had difference in mean stylet length (21.1 μm and 23.4 μm respectively), hyaline tail terminal length (28.3 μm and 24.2 μm respectively) and shape of stylet knob. Highest mean value of vulval basin-anus distance (65.3 μm), number of cuticular ridges between vulval basin-anus (18.4) and Granek’s ratio (4.0 μm) was recorded in G. rostochiensis than G. pallida. Therefore, the present study will help to take appropriate and region specific PCN management decisions according to species dominance in that area.
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| first_indexed | 2024-04-10T15:26:18Z |
| format | Article |
| id | doaj.art-d634a0aa201b4310a6330938a99465ef |
| institution | Directory Open Access Journal |
| issn | 0019-5022 2394-3319 |
| language | English |
| last_indexed | 2024-04-10T15:26:18Z |
| publishDate | 2020-03-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | The Indian Journal of Agricultural Sciences |
| spelling | doaj.art-d634a0aa201b4310a6330938a99465ef2023-02-14T09:14:22ZengIndian Council of Agricultural ResearchThe Indian Journal of Agricultural Sciences0019-50222394-33192020-03-0190210.56093/ijas.v90i2.99000Morphological and molecular characterization of potato cyst nematode populations from The NilgirisAARTI BAIRWA0VENKATASALAM E P1JEEVALATHA A2PRIYANK H M3TANUJA BUCKSETH4JENIFER A5SANJEEV SHARMA6R K SINGH7S K CHAKRABARTI8Scientist, ICAR-Central Potato Research Institute, Shimla, Himachal Pradesh 171 001, IndiaPrincipal Scientist, ICAR-Central Potato Research Institute, Shimla, Himachal Pradesh 171 001, IndiaScientist, ICAR-IISR, Kozhikode, KeralaScientist, ICAR-CPRS, Muthorai, OotyScientist, ICAR-Central Potato Research Institute, Shimla, Himachal Pradesh 171 001, IndiaScientist, ICAR-CPRS, Muthorai, OotyPrincipal Scientist, ICAR-Central Potato Research Institute, Shimla, Himachal Pradesh 171 001, IndiaPrincipal Scientist, ICAR-Central Potato Research Institute, Shimla, Himachal Pradesh 171 001, IndiaPrincipal Scientist, ICAR-Central Potato Research Institute, Shimla, Himachal Pradesh 171 001, India Quarantine species of potato cyst nematodes (PCN), Globodera rostochiensis and G. pallida, were reported from Nilgiris during 1961. The studies were carried out at ICAR-CPRS, Muthorai, Ooty and ICAR-CPRI, Shimla during 2015–17. To investigate the distribution of PCN, soil samples were collected from potato growing areas of Nilgiris and were identified based on morphological criteria and ITS-1 region. Molecular characterization using ITS-1 region specific primers revealed the presence of pure population of G. rostochiensis in 50% of the samples, G. pallida in 10.7% of the samples, mixed population in 28.6% of the samples and absence of both the species in 10.7% of the samples. The phylogenetic analysis inferred by the sequence of the ITS-1 region confirmed 92–100% genetic similarities in Globodera spp. Seventeen isolates of G. rostochiensis showed 92–99% genetic similarity and rest four 92–100% similarities. Whereas, genetic similarity among the ten isolates of G. pallida was 96.1–99.4%. In the morphometric characters J2s of G. rostochiensis exhibited shorter body length (459.8 μm) than G. pallida (493.7 μm). G. rostochiensis and G. pallida had difference in mean stylet length (21.1 μm and 23.4 μm respectively), hyaline tail terminal length (28.3 μm and 24.2 μm respectively) and shape of stylet knob. Highest mean value of vulval basin-anus distance (65.3 μm), number of cuticular ridges between vulval basin-anus (18.4) and Granek’s ratio (4.0 μm) was recorded in G. rostochiensis than G. pallida. Therefore, the present study will help to take appropriate and region specific PCN management decisions according to species dominance in that area. https://epubs.icar.org.in/index.php/IJAgS/article/view/99000G. rostochiensisG. pallidaMolecular characterizationMorphologyITS-1 region |
| spellingShingle | AARTI BAIRWA VENKATASALAM E P JEEVALATHA A PRIYANK H M TANUJA BUCKSETH JENIFER A SANJEEV SHARMA R K SINGH S K CHAKRABARTI Morphological and molecular characterization of potato cyst nematode populations from The Nilgiris The Indian Journal of Agricultural Sciences G. rostochiensis G. pallida Molecular characterization Morphology ITS-1 region |
| title | Morphological and molecular characterization of potato cyst nematode populations from The Nilgiris |
| title_full | Morphological and molecular characterization of potato cyst nematode populations from The Nilgiris |
| title_fullStr | Morphological and molecular characterization of potato cyst nematode populations from The Nilgiris |
| title_full_unstemmed | Morphological and molecular characterization of potato cyst nematode populations from The Nilgiris |
| title_short | Morphological and molecular characterization of potato cyst nematode populations from The Nilgiris |
| title_sort | morphological and molecular characterization of potato cyst nematode populations from the nilgiris |
| topic | G. rostochiensis G. pallida Molecular characterization Morphology ITS-1 region |
| url | https://epubs.icar.org.in/index.php/IJAgS/article/view/99000 |
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