Protective Effect of SGK1 in Rat Hippocampal Neurons Subjected to Ischemia Reperfusion
Background/Aims: To investigate the protective effect of SGK1 (serum- and glucocorticoid-inducible protein kinase 1) in rat hippocampal neurons in vitro and in vivo following ischemia reperfusion (I/R). Methods: Isolated rat hippocampal neurons were subjected to 2 h of oxygen and glucose deprivation...
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| Format: | Article |
| Language: | English |
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Cell Physiol Biochem Press GmbH & Co KG
2014-07-01
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| Series: | Cellular Physiology and Biochemistry |
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| Online Access: | http://www.karger.com/Article/FullText/363000 |
| _version_ | 1818412451849306112 |
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| author | Wei Zhang Chuan yun Qian Shu qing Li |
| author_facet | Wei Zhang Chuan yun Qian Shu qing Li |
| author_sort | Wei Zhang |
| collection | DOAJ |
| description | Background/Aims: To investigate the protective effect of SGK1 (serum- and glucocorticoid-inducible protein kinase 1) in rat hippocampal neurons in vitro and in vivo following ischemia reperfusion (I/R). Methods: Isolated rat hippocampal neurons were subjected to 2 h of oxygen and glucose deprivation (OGD) then returned to normoxic conditions for 10, 30 or 60 min. Cell apoptosis and protein expression of SGK1 were analyzed. To examine SGK1 function, we overexpressed SGK1 in rat hippocampal neurons. Finally we examined the involvement of PI3K/Akt/GSK3β signaling by treating the cells (untransfected or transfected with expression vector encoding SGK1) with the PI3K inhibitor LY294002. Findings were confirmed in vivo in a rat model of middle cerebral artery occlusion. Results: I/R caused a time-dependent increase in apoptosis, both in vitro and in vivo. SGK1 protein levels decreased significantly under the same conditions. Overexpression of SGK1 reduced apoptosis following OGD or I/R compared to cells transfected with empty vector and subjected to the same treatment, or sham-operated animals. Addition of LY294002 revealed that the action of SGK1 in suppressing apoptosis was mediated by the PI3K/Akt/GSK3β pathway. Conclusion: SGK1 plays a protective role in ischemia reperfusion in rat hippocampal neurons, exerting its effects via the PI3K/Akt/GSK3β pathway. |
| first_indexed | 2024-12-14T10:47:31Z |
| format | Article |
| id | doaj.art-d638ae29efcb4aa3b569f5851c4a2796 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-14T10:47:31Z |
| publishDate | 2014-07-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-d638ae29efcb4aa3b569f5851c4a27962022-12-21T23:05:22ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782014-07-0134229931210.1159/000363000363000Protective Effect of SGK1 in Rat Hippocampal Neurons Subjected to Ischemia ReperfusionWei ZhangChuan yun QianShu qing LiBackground/Aims: To investigate the protective effect of SGK1 (serum- and glucocorticoid-inducible protein kinase 1) in rat hippocampal neurons in vitro and in vivo following ischemia reperfusion (I/R). Methods: Isolated rat hippocampal neurons were subjected to 2 h of oxygen and glucose deprivation (OGD) then returned to normoxic conditions for 10, 30 or 60 min. Cell apoptosis and protein expression of SGK1 were analyzed. To examine SGK1 function, we overexpressed SGK1 in rat hippocampal neurons. Finally we examined the involvement of PI3K/Akt/GSK3β signaling by treating the cells (untransfected or transfected with expression vector encoding SGK1) with the PI3K inhibitor LY294002. Findings were confirmed in vivo in a rat model of middle cerebral artery occlusion. Results: I/R caused a time-dependent increase in apoptosis, both in vitro and in vivo. SGK1 protein levels decreased significantly under the same conditions. Overexpression of SGK1 reduced apoptosis following OGD or I/R compared to cells transfected with empty vector and subjected to the same treatment, or sham-operated animals. Addition of LY294002 revealed that the action of SGK1 in suppressing apoptosis was mediated by the PI3K/Akt/GSK3β pathway. Conclusion: SGK1 plays a protective role in ischemia reperfusion in rat hippocampal neurons, exerting its effects via the PI3K/Akt/GSK3β pathway.http://www.karger.com/Article/FullText/363000SGK1ApoptosisNecrosisNeuronsHippocampusIschemia reperfusion |
| spellingShingle | Wei Zhang Chuan yun Qian Shu qing Li Protective Effect of SGK1 in Rat Hippocampal Neurons Subjected to Ischemia Reperfusion Cellular Physiology and Biochemistry SGK1 Apoptosis Necrosis Neurons Hippocampus Ischemia reperfusion |
| title | Protective Effect of SGK1 in Rat Hippocampal Neurons Subjected to Ischemia Reperfusion |
| title_full | Protective Effect of SGK1 in Rat Hippocampal Neurons Subjected to Ischemia Reperfusion |
| title_fullStr | Protective Effect of SGK1 in Rat Hippocampal Neurons Subjected to Ischemia Reperfusion |
| title_full_unstemmed | Protective Effect of SGK1 in Rat Hippocampal Neurons Subjected to Ischemia Reperfusion |
| title_short | Protective Effect of SGK1 in Rat Hippocampal Neurons Subjected to Ischemia Reperfusion |
| title_sort | protective effect of sgk1 in rat hippocampal neurons subjected to ischemia reperfusion |
| topic | SGK1 Apoptosis Necrosis Neurons Hippocampus Ischemia reperfusion |
| url | http://www.karger.com/Article/FullText/363000 |
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