Calycosin Orchestrates Osteogenesis of Danggui Buxue Tang in Cultured Osteoblasts: Evaluating the Mechanism of Action by Omics and Chemical Knock-out Methodologies
Danggui Buxue Tang (DBT), an ancient Chinese herbal decoction commonly used to mitigate menopausal osteoporosis, contains two herbs: Astragali Radix (AR) and Angelicae Sinensis Radix (ASR). The exact efficacy of individual chemical(s) within DBT, or in any herbal mixture, is hard to be revealed. Cal...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-02-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fphar.2018.00036/full |
| _version_ | 1818306947040935936 |
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| author | Amy G. W. Gong Amy G. W. Gong Ran Duan Ran Duan Huai Y. Wang Huai Y. Wang Tina T. X. Dong Tina T. X. Dong Karl W. K. Tsim Karl W. K. Tsim |
| author_facet | Amy G. W. Gong Amy G. W. Gong Ran Duan Ran Duan Huai Y. Wang Huai Y. Wang Tina T. X. Dong Tina T. X. Dong Karl W. K. Tsim Karl W. K. Tsim |
| author_sort | Amy G. W. Gong |
| collection | DOAJ |
| description | Danggui Buxue Tang (DBT), an ancient Chinese herbal decoction commonly used to mitigate menopausal osteoporosis, contains two herbs: Astragali Radix (AR) and Angelicae Sinensis Radix (ASR). The exact efficacy of individual chemical(s) within DBT, or in any herbal mixture, is hard to be revealed. Calycosin and ferulic acid have been reported to be the predominant chemicals found within DBT, and its roles in regulating osteoblastic differentiation have been proposed here. To probe the roles of calycosin and ferulic acid, these chemicals were specifically depleted from the DBT extracts. Here, calycosin-depleted DBT (DBTΔcal) and ferulic acid-depleted DBT (DBTΔfa), generated by semi-preparative HPLC, were coupled with RNA-seq and metabolomics analyses to reveal the synergistic functions of individual chemicals within a complex herbal mixture. The expressions of osteogenic differentiation markers were significantly increased under the treatments of DBT and DBTΔfa. The DBT-induced genes were markedly reduced in the absent of calycosin, i.e., DBTΔcal. In cultured osteoblasts, the DBT-activated Wnt/β-catenin and MAPK/Erk and signaling pathways were greatly affected when calycosin was depleted. By metabolomics analysis in DBT-treated osteoblasts, the profile of metabolites triggered by DBTΔcal showed distinction to that of DBT and/or DBTΔfa. Thus, our findings indicated that calycosin, rather than ferulic acid, could be an indispensable chemical in DBT to orchestrate multi-components of DBT in achieving maximal osteogenic properties. |
| first_indexed | 2024-12-13T06:50:34Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-13T06:50:34Z |
| publishDate | 2018-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-d63b27b208f14949bbf853cacf40caec2022-12-21T23:56:09ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122018-02-01910.3389/fphar.2018.00036315538Calycosin Orchestrates Osteogenesis of Danggui Buxue Tang in Cultured Osteoblasts: Evaluating the Mechanism of Action by Omics and Chemical Knock-out MethodologiesAmy G. W. Gong0Amy G. W. Gong1Ran Duan2Ran Duan3Huai Y. Wang4Huai Y. Wang5Tina T. X. Dong6Tina T. X. Dong7Karl W. K. Tsim8Karl W. K. Tsim9HKUST Shenzhen Research Institute, Shenzhen, ChinaDivision of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, Hong KongHKUST Shenzhen Research Institute, Shenzhen, ChinaDivision of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, Hong KongHKUST Shenzhen Research Institute, Shenzhen, ChinaDivision of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, Hong KongHKUST Shenzhen Research Institute, Shenzhen, ChinaDivision of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, Hong KongHKUST Shenzhen Research Institute, Shenzhen, ChinaDivision of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, Hong KongDanggui Buxue Tang (DBT), an ancient Chinese herbal decoction commonly used to mitigate menopausal osteoporosis, contains two herbs: Astragali Radix (AR) and Angelicae Sinensis Radix (ASR). The exact efficacy of individual chemical(s) within DBT, or in any herbal mixture, is hard to be revealed. Calycosin and ferulic acid have been reported to be the predominant chemicals found within DBT, and its roles in regulating osteoblastic differentiation have been proposed here. To probe the roles of calycosin and ferulic acid, these chemicals were specifically depleted from the DBT extracts. Here, calycosin-depleted DBT (DBTΔcal) and ferulic acid-depleted DBT (DBTΔfa), generated by semi-preparative HPLC, were coupled with RNA-seq and metabolomics analyses to reveal the synergistic functions of individual chemicals within a complex herbal mixture. The expressions of osteogenic differentiation markers were significantly increased under the treatments of DBT and DBTΔfa. The DBT-induced genes were markedly reduced in the absent of calycosin, i.e., DBTΔcal. In cultured osteoblasts, the DBT-activated Wnt/β-catenin and MAPK/Erk and signaling pathways were greatly affected when calycosin was depleted. By metabolomics analysis in DBT-treated osteoblasts, the profile of metabolites triggered by DBTΔcal showed distinction to that of DBT and/or DBTΔfa. Thus, our findings indicated that calycosin, rather than ferulic acid, could be an indispensable chemical in DBT to orchestrate multi-components of DBT in achieving maximal osteogenic properties.http://journal.frontiersin.org/article/10.3389/fphar.2018.00036/fullAstragali RadixAngelicae Sinensis RadixDanggui Buxue TangRNA-seqmetabolomicsosteoblast |
| spellingShingle | Amy G. W. Gong Amy G. W. Gong Ran Duan Ran Duan Huai Y. Wang Huai Y. Wang Tina T. X. Dong Tina T. X. Dong Karl W. K. Tsim Karl W. K. Tsim Calycosin Orchestrates Osteogenesis of Danggui Buxue Tang in Cultured Osteoblasts: Evaluating the Mechanism of Action by Omics and Chemical Knock-out Methodologies Frontiers in Pharmacology Astragali Radix Angelicae Sinensis Radix Danggui Buxue Tang RNA-seq metabolomics osteoblast |
| title | Calycosin Orchestrates Osteogenesis of Danggui Buxue Tang in Cultured Osteoblasts: Evaluating the Mechanism of Action by Omics and Chemical Knock-out Methodologies |
| title_full | Calycosin Orchestrates Osteogenesis of Danggui Buxue Tang in Cultured Osteoblasts: Evaluating the Mechanism of Action by Omics and Chemical Knock-out Methodologies |
| title_fullStr | Calycosin Orchestrates Osteogenesis of Danggui Buxue Tang in Cultured Osteoblasts: Evaluating the Mechanism of Action by Omics and Chemical Knock-out Methodologies |
| title_full_unstemmed | Calycosin Orchestrates Osteogenesis of Danggui Buxue Tang in Cultured Osteoblasts: Evaluating the Mechanism of Action by Omics and Chemical Knock-out Methodologies |
| title_short | Calycosin Orchestrates Osteogenesis of Danggui Buxue Tang in Cultured Osteoblasts: Evaluating the Mechanism of Action by Omics and Chemical Knock-out Methodologies |
| title_sort | calycosin orchestrates osteogenesis of danggui buxue tang in cultured osteoblasts evaluating the mechanism of action by omics and chemical knock out methodologies |
| topic | Astragali Radix Angelicae Sinensis Radix Danggui Buxue Tang RNA-seq metabolomics osteoblast |
| url | http://journal.frontiersin.org/article/10.3389/fphar.2018.00036/full |
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