Cytogenetic Characterization and Metabolomic Differences of Full-Sib Progenies of <i>Saccharum</i> spp.
Sugarcane smut is a worldwide fungal disease. Disease resistance breeding is the most economical and effective measure to prevent and control sugarcane smut. The cytogenetic characteristics and metabolomic differences of sugarcane F<sub>1</sub>s are closely related to disease resistance....
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MDPI AG
2023-02-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/4/810 |
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| author | Yi Wang Ru Li Baoshan Chen |
| author_facet | Yi Wang Ru Li Baoshan Chen |
| author_sort | Yi Wang |
| collection | DOAJ |
| description | Sugarcane smut is a worldwide fungal disease. Disease resistance breeding is the most economical and effective measure to prevent and control sugarcane smut. The cytogenetic characteristics and metabolomic differences of sugarcane F<sub>1</sub>s are closely related to disease resistance. Zhongzhe 1 and G160 sugarcane from the same parents (ROC25 and Yunzhe89-7) were used; the plants were grown in accordance with the barrel method. When the seedlings had 4–5 leaves, genomic in situ hybridization (GISH) was performed; digoxigenin (DIG)-labeled female parental (ROC25)DNA and biotin-labeled male parental (Yunzhe89-7) DNA were used as probes, and the karyotypes of two hybrids were analyzed. The new sugarcane smut-resistant variety (Zhongzhe 1) and the susceptible variety (G160) derived from the same parent were analyzed via gas chromatography—mass spectrometry technology (GC–MS) to compare the metabolomic differences between them. GISH analysis revealed that the chromosome ploidy number of Zhongzhe 1 sugarcane and G160 sugarcane were 114 and 110, respectively. However, the two contain different numbers of chromosomes from the female (ROC25) and male (Yunzhe89-7) parents. Moreover, 258 significantly changed metabolites were identified in smut-resistant Zhongzhe 1, as compared with the smut-susceptible G160 sugarcane: 56 flavonoids, 52 phenolic acids, 30 lipids, 26 organic acids, 26 amino acids and derivatives, 19 nucleotides and derivatives, 5 alkaloids, 9 terpenoids, and 35 others. Multivariate statistical analysis revealed a distinct difference in metabolic pathways between Zhongzhe 1 sugarcane and G160, and both of these varieties had unique functional metabolites. Differences in chromosome composition may constitute the genetic basis for the difference in resistance to smut disease between Zhongzhe 1 sugarcane and G160 sugarcane, and a high accumulation of flavonoids, lipids, terpenoids and tannins may constitute the basis of resistance to smut disease for the Zhongzhe 1 variety. |
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| spelling | doaj.art-d63bfc1ec310448b8ecb57c30eb6cc722023-11-16T22:47:47ZengMDPI AGPlants2223-77472023-02-0112481010.3390/plants12040810Cytogenetic Characterization and Metabolomic Differences of Full-Sib Progenies of <i>Saccharum</i> spp.Yi Wang0Ru Li1Baoshan Chen2State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530004, ChinaSugarcane smut is a worldwide fungal disease. Disease resistance breeding is the most economical and effective measure to prevent and control sugarcane smut. The cytogenetic characteristics and metabolomic differences of sugarcane F<sub>1</sub>s are closely related to disease resistance. Zhongzhe 1 and G160 sugarcane from the same parents (ROC25 and Yunzhe89-7) were used; the plants were grown in accordance with the barrel method. When the seedlings had 4–5 leaves, genomic in situ hybridization (GISH) was performed; digoxigenin (DIG)-labeled female parental (ROC25)DNA and biotin-labeled male parental (Yunzhe89-7) DNA were used as probes, and the karyotypes of two hybrids were analyzed. The new sugarcane smut-resistant variety (Zhongzhe 1) and the susceptible variety (G160) derived from the same parent were analyzed via gas chromatography—mass spectrometry technology (GC–MS) to compare the metabolomic differences between them. GISH analysis revealed that the chromosome ploidy number of Zhongzhe 1 sugarcane and G160 sugarcane were 114 and 110, respectively. However, the two contain different numbers of chromosomes from the female (ROC25) and male (Yunzhe89-7) parents. Moreover, 258 significantly changed metabolites were identified in smut-resistant Zhongzhe 1, as compared with the smut-susceptible G160 sugarcane: 56 flavonoids, 52 phenolic acids, 30 lipids, 26 organic acids, 26 amino acids and derivatives, 19 nucleotides and derivatives, 5 alkaloids, 9 terpenoids, and 35 others. Multivariate statistical analysis revealed a distinct difference in metabolic pathways between Zhongzhe 1 sugarcane and G160, and both of these varieties had unique functional metabolites. Differences in chromosome composition may constitute the genetic basis for the difference in resistance to smut disease between Zhongzhe 1 sugarcane and G160 sugarcane, and a high accumulation of flavonoids, lipids, terpenoids and tannins may constitute the basis of resistance to smut disease for the Zhongzhe 1 variety.https://www.mdpi.com/2223-7747/12/4/810<i>Saccharum</i> spp.smut diseasegenomic in situ hybridizationmetabolomicsdisease resistance |
| spellingShingle | Yi Wang Ru Li Baoshan Chen Cytogenetic Characterization and Metabolomic Differences of Full-Sib Progenies of <i>Saccharum</i> spp. Plants <i>Saccharum</i> spp. smut disease genomic in situ hybridization metabolomics disease resistance |
| title | Cytogenetic Characterization and Metabolomic Differences of Full-Sib Progenies of <i>Saccharum</i> spp. |
| title_full | Cytogenetic Characterization and Metabolomic Differences of Full-Sib Progenies of <i>Saccharum</i> spp. |
| title_fullStr | Cytogenetic Characterization and Metabolomic Differences of Full-Sib Progenies of <i>Saccharum</i> spp. |
| title_full_unstemmed | Cytogenetic Characterization and Metabolomic Differences of Full-Sib Progenies of <i>Saccharum</i> spp. |
| title_short | Cytogenetic Characterization and Metabolomic Differences of Full-Sib Progenies of <i>Saccharum</i> spp. |
| title_sort | cytogenetic characterization and metabolomic differences of full sib progenies of i saccharum i spp |
| topic | <i>Saccharum</i> spp. smut disease genomic in situ hybridization metabolomics disease resistance |
| url | https://www.mdpi.com/2223-7747/12/4/810 |
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