Western diet-induced ultrastructural changes in mouse pancreatic acinar cells
Mouse models of diet-induced type 2 diabetes mellitus provide powerful tools for studying the structural and physiological changes that are related to the disease progression. In this study, diabetic-like glucose dysregulation was induced in mice by feeding them a western diet, and light and transmi...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-03-01
|
| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2024.1380564/full |
| _version_ | 1797263089358340096 |
|---|---|
| author | Saška Lipovšek Saška Lipovšek Saška Lipovšek Saška Lipovšek Jurij Dolenšek Jurij Dolenšek Barbara Dariš Ismael Valladolid-Acebes Tanja Vajs Gerd Leitinger Andraž Stožer Maša Skelin Klemen |
| author_facet | Saška Lipovšek Saška Lipovšek Saška Lipovšek Saška Lipovšek Jurij Dolenšek Jurij Dolenšek Barbara Dariš Ismael Valladolid-Acebes Tanja Vajs Gerd Leitinger Andraž Stožer Maša Skelin Klemen |
| author_sort | Saška Lipovšek |
| collection | DOAJ |
| description | Mouse models of diet-induced type 2 diabetes mellitus provide powerful tools for studying the structural and physiological changes that are related to the disease progression. In this study, diabetic-like glucose dysregulation was induced in mice by feeding them a western diet, and light and transmission electron microscopy were used to study the ultrastructural changes in the pancreatic acinar cells. Acinar necrosis and vacuolization of the cytoplasm were the most prominent features. Furthermore, we observed intracellular and extracellular accumulation of lipid compounds in the form of lipid droplets, structural enlargement of the cisternae of the rough endoplasmic reticulum (RER), and altered mitochondrial morphology, with mitochondria lacking the typical organization of the inner membrane. Last, autophagic structures, i.e., autophagosomes, autolysosomes, and residual bodies, were abundant within the acinar cells of western diet-fed mice, and the autolysosomes contained lipids and material of varying electron density. While diets inducing obesity and type 2 diabetes are clearly associated with structural changes and dysfunction of the endocrine pancreas, we here demonstrate the strong effect of dietary intervention on the structure of acinar cells in the exocrine part of the organ before detectable changes in plasma amylase activity, which may help us better understand the development of non-alcoholic fatty pancreas disease and its association with endo- and exocrine dysfunction. |
| first_indexed | 2024-04-25T00:07:28Z |
| format | Article |
| id | doaj.art-d63ce1fc53084f619142e10ecca7589b |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-04-25T00:07:28Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-d63ce1fc53084f619142e10ecca7589b2024-03-14T04:51:36ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2024-03-011210.3389/fcell.2024.13805641380564Western diet-induced ultrastructural changes in mouse pancreatic acinar cellsSaška Lipovšek0Saška Lipovšek1Saška Lipovšek2Saška Lipovšek3Jurij Dolenšek4Jurij Dolenšek5Barbara Dariš6Ismael Valladolid-Acebes7Tanja Vajs8Gerd Leitinger9Andraž Stožer10Maša Skelin Klemen11Faculty of Medicine, University of Maribor, Maribor, SloveniaDepartment of Biology, Faculty of Natural Sciences and Mathematics, University of Maribor, Maribor, SloveniaGottfried Schatz Research Center, Division of Cell Biology, Histology and Embryology, Medical University of Graz, Graz, AustriaFaculty of Chemistry and Chemical Engineering, University of Maribor, Maribor, SloveniaFaculty of Medicine, University of Maribor, Maribor, SloveniaDepartment of Biology, Faculty of Natural Sciences and Mathematics, University of Maribor, Maribor, SloveniaFaculty of Medicine, University of Maribor, Maribor, SloveniaThe Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital, Stockholm, SwedenFaculty of Medicine, University of Maribor, Maribor, SloveniaGottfried Schatz Research Center, Division of Cell Biology, Histology and Embryology, Medical University of Graz, Graz, AustriaFaculty of Medicine, University of Maribor, Maribor, SloveniaFaculty of Medicine, University of Maribor, Maribor, SloveniaMouse models of diet-induced type 2 diabetes mellitus provide powerful tools for studying the structural and physiological changes that are related to the disease progression. In this study, diabetic-like glucose dysregulation was induced in mice by feeding them a western diet, and light and transmission electron microscopy were used to study the ultrastructural changes in the pancreatic acinar cells. Acinar necrosis and vacuolization of the cytoplasm were the most prominent features. Furthermore, we observed intracellular and extracellular accumulation of lipid compounds in the form of lipid droplets, structural enlargement of the cisternae of the rough endoplasmic reticulum (RER), and altered mitochondrial morphology, with mitochondria lacking the typical organization of the inner membrane. Last, autophagic structures, i.e., autophagosomes, autolysosomes, and residual bodies, were abundant within the acinar cells of western diet-fed mice, and the autolysosomes contained lipids and material of varying electron density. While diets inducing obesity and type 2 diabetes are clearly associated with structural changes and dysfunction of the endocrine pancreas, we here demonstrate the strong effect of dietary intervention on the structure of acinar cells in the exocrine part of the organ before detectable changes in plasma amylase activity, which may help us better understand the development of non-alcoholic fatty pancreas disease and its association with endo- and exocrine dysfunction.https://www.frontiersin.org/articles/10.3389/fcell.2024.1380564/fullacinar cellsautophagylipid dropletsmitochondrianecrotic cellsrough endoplasmic reticulum |
| spellingShingle | Saška Lipovšek Saška Lipovšek Saška Lipovšek Saška Lipovšek Jurij Dolenšek Jurij Dolenšek Barbara Dariš Ismael Valladolid-Acebes Tanja Vajs Gerd Leitinger Andraž Stožer Maša Skelin Klemen Western diet-induced ultrastructural changes in mouse pancreatic acinar cells Frontiers in Cell and Developmental Biology acinar cells autophagy lipid droplets mitochondria necrotic cells rough endoplasmic reticulum |
| title | Western diet-induced ultrastructural changes in mouse pancreatic acinar cells |
| title_full | Western diet-induced ultrastructural changes in mouse pancreatic acinar cells |
| title_fullStr | Western diet-induced ultrastructural changes in mouse pancreatic acinar cells |
| title_full_unstemmed | Western diet-induced ultrastructural changes in mouse pancreatic acinar cells |
| title_short | Western diet-induced ultrastructural changes in mouse pancreatic acinar cells |
| title_sort | western diet induced ultrastructural changes in mouse pancreatic acinar cells |
| topic | acinar cells autophagy lipid droplets mitochondria necrotic cells rough endoplasmic reticulum |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2024.1380564/full |
| work_keys_str_mv | AT saskalipovsek westerndietinducedultrastructuralchangesinmousepancreaticacinarcells AT saskalipovsek westerndietinducedultrastructuralchangesinmousepancreaticacinarcells AT saskalipovsek westerndietinducedultrastructuralchangesinmousepancreaticacinarcells AT saskalipovsek westerndietinducedultrastructuralchangesinmousepancreaticacinarcells AT jurijdolensek westerndietinducedultrastructuralchangesinmousepancreaticacinarcells AT jurijdolensek westerndietinducedultrastructuralchangesinmousepancreaticacinarcells AT barbaradaris westerndietinducedultrastructuralchangesinmousepancreaticacinarcells AT ismaelvalladolidacebes westerndietinducedultrastructuralchangesinmousepancreaticacinarcells AT tanjavajs westerndietinducedultrastructuralchangesinmousepancreaticacinarcells AT gerdleitinger westerndietinducedultrastructuralchangesinmousepancreaticacinarcells AT andrazstozer westerndietinducedultrastructuralchangesinmousepancreaticacinarcells AT masaskelinklemen westerndietinducedultrastructuralchangesinmousepancreaticacinarcells |