In vitro cytotoxic and genotoxic analysis of Garcinia humilis crude extract
Garcinia humilis, known commonly as achachairú or bacupari, has great medicinal value. Their fruits have pharmacological, antibacterial, antioxidant, and anticancer properties. Therefore, the objective of the present study was to evaluate the cytotoxicity and genotoxicity of G. humilis crude extract...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Uberlândia
2022-12-01
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| Series: | Bioscience Journal |
| Subjects: | |
| Online Access: | https://seer.ufu.br/index.php/biosciencejournal/article/view/59901 |
| _version_ | 1811169836263276544 |
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| author | Alcione da Silva Arruda Pedro Henrique Alves Machado Ricardo Campos Lino Roberta Camargos de Oliveira José Magno Queiroz Luz Robson José Oliveira Júnior |
| author_facet | Alcione da Silva Arruda Pedro Henrique Alves Machado Ricardo Campos Lino Roberta Camargos de Oliveira José Magno Queiroz Luz Robson José Oliveira Júnior |
| author_sort | Alcione da Silva Arruda |
| collection | DOAJ |
| description | Garcinia humilis, known commonly as achachairú or bacupari, has great medicinal value. Their fruits have pharmacological, antibacterial, antioxidant, and anticancer properties. Therefore, the objective of the present study was to evaluate the cytotoxicity and genotoxicity of G. humilis crude extract in breast tumor cells. Cytotoxicity was determined using the Resazurin reduction assay and genotoxicity by the single cell gel electrophoresis assay (Comet assay) on human MCF-7 cells. Crude extract of G. humilis was cytotoxic only when used at high concentrations (IC50 = 5.084 mg mL-1). The Comet assay showed that the crude extract did not induce genotoxicity at 1 and 5 mg mL-1 but did show signs of DNA fragmentation and DNA fragmentation at 10 mg mL-1. The cytotoxic activity against breast adenocarcinoma cells at high concentrations suggests that this medicinal plant could be used with caution and must be further studied to understand better its therapeutic and toxicological potential in the human body. |
| first_indexed | 2024-04-10T16:48:06Z |
| format | Article |
| id | doaj.art-d63efe49729746869085545108a308af |
| institution | Directory Open Access Journal |
| issn | 1981-3163 |
| language | English |
| last_indexed | 2024-04-10T16:48:06Z |
| publishDate | 2022-12-01 |
| publisher | Universidade Federal de Uberlândia |
| record_format | Article |
| series | Bioscience Journal |
| spelling | doaj.art-d63efe49729746869085545108a308af2023-02-07T19:06:26ZengUniversidade Federal de UberlândiaBioscience Journal1981-31632022-12-0138e38097e3809710.14393/BJ-v38n0a2022-5990132762In vitro cytotoxic and genotoxic analysis of Garcinia humilis crude extractAlcione da Silva Arruda0https://orcid.org/0000-0003-1361-5533Pedro Henrique Alves Machado1https://orcid.org/0000-0002-3029-5618Ricardo Campos Lino2https://orcid.org/0000-0002-7667-0671Roberta Camargos de Oliveira3https://orcid.org/0000-0002-2432-0442José Magno Queiroz Luz4https://orcid.org/0000-0002-5712-7710Robson José Oliveira Júnior5https://orcid.org/0000-0001-8453-3194Universidade Estadual de Goiás Universidade Federal de UberlândiaUniversidade Federal de UberlândiaUniversidade Federal de UberlândiaUniversidade Federal de UberlândiaUniversidade Federal de UberlândiaGarcinia humilis, known commonly as achachairú or bacupari, has great medicinal value. Their fruits have pharmacological, antibacterial, antioxidant, and anticancer properties. Therefore, the objective of the present study was to evaluate the cytotoxicity and genotoxicity of G. humilis crude extract in breast tumor cells. Cytotoxicity was determined using the Resazurin reduction assay and genotoxicity by the single cell gel electrophoresis assay (Comet assay) on human MCF-7 cells. Crude extract of G. humilis was cytotoxic only when used at high concentrations (IC50 = 5.084 mg mL-1). The Comet assay showed that the crude extract did not induce genotoxicity at 1 and 5 mg mL-1 but did show signs of DNA fragmentation and DNA fragmentation at 10 mg mL-1. The cytotoxic activity against breast adenocarcinoma cells at high concentrations suggests that this medicinal plant could be used with caution and must be further studied to understand better its therapeutic and toxicological potential in the human body.https://seer.ufu.br/index.php/biosciencejournal/article/view/59901achachairú.anticancer activityantioxidant activitycomet assay |
| spellingShingle | Alcione da Silva Arruda Pedro Henrique Alves Machado Ricardo Campos Lino Roberta Camargos de Oliveira José Magno Queiroz Luz Robson José Oliveira Júnior In vitro cytotoxic and genotoxic analysis of Garcinia humilis crude extract Bioscience Journal achachairú. anticancer activity antioxidant activity comet assay |
| title | In vitro cytotoxic and genotoxic analysis of Garcinia humilis crude extract |
| title_full | In vitro cytotoxic and genotoxic analysis of Garcinia humilis crude extract |
| title_fullStr | In vitro cytotoxic and genotoxic analysis of Garcinia humilis crude extract |
| title_full_unstemmed | In vitro cytotoxic and genotoxic analysis of Garcinia humilis crude extract |
| title_short | In vitro cytotoxic and genotoxic analysis of Garcinia humilis crude extract |
| title_sort | in vitro cytotoxic and genotoxic analysis of garcinia humilis crude extract |
| topic | achachairú. anticancer activity antioxidant activity comet assay |
| url | https://seer.ufu.br/index.php/biosciencejournal/article/view/59901 |
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