Wound healing approach based on excretory-secretory product and lysate of liver flukes
Abstract Exogenous bioactive peptides are considered promising for the wound healing therapy in humans. In this regard, parasitic trematodes proteins may potentially become a new perspective agents. Foodborne trematode Opisthorchis felineus is widespread in Europe and has the ability to stimulate pr...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-26275-y |
| _version_ | 1811292441396903936 |
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| author | Anna V. Kovner Alena A. Tarasenko Oxana Zaparina Olga V. Tikhonova Maria Y. Pakharukova Viatcheslav A. Mordvinov |
| author_facet | Anna V. Kovner Alena A. Tarasenko Oxana Zaparina Olga V. Tikhonova Maria Y. Pakharukova Viatcheslav A. Mordvinov |
| author_sort | Anna V. Kovner |
| collection | DOAJ |
| description | Abstract Exogenous bioactive peptides are considered promising for the wound healing therapy in humans. In this regard, parasitic trematodes proteins may potentially become a new perspective agents. Foodborne trematode Opisthorchis felineus is widespread in Europe and has the ability to stimulate proliferation of bile duct epithelium. In this study, we investigated skin wound healing potential of O. felineus proteins in mouse model. C57Bl/6 mice were inflicted with superficial wounds with 8 mm diameter. Experimental groups included several non-specific controls and specific treatment groups (excretory-secretory product and lysate). After 10 days of the experiment, the percentage of wound healing in the specific treatment groups significantly exceeded the control values. We also found that wound treatment with excretory-secretory product and worm lysate resulted in: (i) inflammation reducing, (ii) vascular response modulating, (iii) type 1 collagen deposition promoting dermal ECM remodeling. An additional proteomic analysis of excretory-secretory product and worm lysate samples was revealed 111 common proteins. The obtained data indicate a high wound-healing potential of liver fluke proteins and open prospects for further research as new therapeutic approaches. |
| first_indexed | 2024-04-13T04:45:27Z |
| format | Article |
| id | doaj.art-d6406bbbbca74d2396efb94dfb12bf2a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T04:45:27Z |
| publishDate | 2022-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-d6406bbbbca74d2396efb94dfb12bf2a2022-12-22T03:01:51ZengNature PortfolioScientific Reports2045-23222022-12-0112111510.1038/s41598-022-26275-yWound healing approach based on excretory-secretory product and lysate of liver flukesAnna V. Kovner0Alena A. Tarasenko1Oxana Zaparina2Olga V. Tikhonova3Maria Y. Pakharukova4Viatcheslav A. Mordvinov5Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG SB RAS)Department of Natural Sciences, Novosibirsk State UniversityInstitute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG SB RAS)Institute of Biomedical Chemistry (IBMC) RUInstitute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG SB RAS)Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG SB RAS)Abstract Exogenous bioactive peptides are considered promising for the wound healing therapy in humans. In this regard, parasitic trematodes proteins may potentially become a new perspective agents. Foodborne trematode Opisthorchis felineus is widespread in Europe and has the ability to stimulate proliferation of bile duct epithelium. In this study, we investigated skin wound healing potential of O. felineus proteins in mouse model. C57Bl/6 mice were inflicted with superficial wounds with 8 mm diameter. Experimental groups included several non-specific controls and specific treatment groups (excretory-secretory product and lysate). After 10 days of the experiment, the percentage of wound healing in the specific treatment groups significantly exceeded the control values. We also found that wound treatment with excretory-secretory product and worm lysate resulted in: (i) inflammation reducing, (ii) vascular response modulating, (iii) type 1 collagen deposition promoting dermal ECM remodeling. An additional proteomic analysis of excretory-secretory product and worm lysate samples was revealed 111 common proteins. The obtained data indicate a high wound-healing potential of liver fluke proteins and open prospects for further research as new therapeutic approaches.https://doi.org/10.1038/s41598-022-26275-y |
| spellingShingle | Anna V. Kovner Alena A. Tarasenko Oxana Zaparina Olga V. Tikhonova Maria Y. Pakharukova Viatcheslav A. Mordvinov Wound healing approach based on excretory-secretory product and lysate of liver flukes Scientific Reports |
| title | Wound healing approach based on excretory-secretory product and lysate of liver flukes |
| title_full | Wound healing approach based on excretory-secretory product and lysate of liver flukes |
| title_fullStr | Wound healing approach based on excretory-secretory product and lysate of liver flukes |
| title_full_unstemmed | Wound healing approach based on excretory-secretory product and lysate of liver flukes |
| title_short | Wound healing approach based on excretory-secretory product and lysate of liver flukes |
| title_sort | wound healing approach based on excretory secretory product and lysate of liver flukes |
| url | https://doi.org/10.1038/s41598-022-26275-y |
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