Transcriptome datasets supply basic gene information for RNAi pest management and gene functional studies in Nephotettix cincticeps (Uhler)
RNA interference (RNAi) technology has the potential to be used in pest management in crop production. Here, the transcriptome of Nephotettix cincticeps (Uhler) was deeply sequenced to investigate the systematic RNAi mechanism and candidate genes for dsRNA feeding. In our datasets, a total of 81 225...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-04-01
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| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311915611339 |
| _version_ | 1819069631662391296 |
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| author | Tai-yu CHEN Ji-xiang HOU Yong-jun LIN |
| author_facet | Tai-yu CHEN Ji-xiang HOU Yong-jun LIN |
| author_sort | Tai-yu CHEN |
| collection | DOAJ |
| description | RNA interference (RNAi) technology has the potential to be used in pest management in crop production. Here, the transcriptome of Nephotettix cincticeps (Uhler) was deeply sequenced to investigate the systematic RNAi mechanism and candidate genes for dsRNA feeding. In our datasets, a total of 81 225 transcripts were obtained with the length from 150 bp to about 4.2 kb. Almost all the genes related to the RNAi core pathway were proved to be present in N. cincticeps transcriptome. Two transcripts that respectively encode a systemic interference defective (SID) were identified in our database, indicating that the systematic RNAi pathway can function effectively in N.cincticeps. Our datasets not only supply basic gene information for the studies of gene expression and functions in N. cincticeps, such as the control genes for gene expression analysis, but also provide candidate genes for RNAi pest management, such as the genes that encode P450 monooxygenase, V-ATPase and chitin synthase. |
| first_indexed | 2024-12-21T16:53:07Z |
| format | Article |
| id | doaj.art-d6420395fc71435f874d449d2b581652 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-21T16:53:07Z |
| publishDate | 2016-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-d6420395fc71435f874d449d2b5816522022-12-21T18:56:50ZengElsevierJournal of Integrative Agriculture2095-31192016-04-01154840847Transcriptome datasets supply basic gene information for RNAi pest management and gene functional studies in Nephotettix cincticeps (Uhler)Tai-yu CHEN0Ji-xiang HOU1Yong-jun LIN2National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P.R.China; College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, P.R.China; CHEN Tai-yu, Tel: +86-27-87280516National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P.R.ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P.R.China; Correspondence LIN Yong-jun, Tel: +86-27-87281719RNA interference (RNAi) technology has the potential to be used in pest management in crop production. Here, the transcriptome of Nephotettix cincticeps (Uhler) was deeply sequenced to investigate the systematic RNAi mechanism and candidate genes for dsRNA feeding. In our datasets, a total of 81 225 transcripts were obtained with the length from 150 bp to about 4.2 kb. Almost all the genes related to the RNAi core pathway were proved to be present in N. cincticeps transcriptome. Two transcripts that respectively encode a systemic interference defective (SID) were identified in our database, indicating that the systematic RNAi pathway can function effectively in N.cincticeps. Our datasets not only supply basic gene information for the studies of gene expression and functions in N. cincticeps, such as the control genes for gene expression analysis, but also provide candidate genes for RNAi pest management, such as the genes that encode P450 monooxygenase, V-ATPase and chitin synthase.http://www.sciencedirect.com/science/article/pii/S2095311915611339Nephotettix cincticepstranscriptomeRNA interferencehouse-keeping genes |
| spellingShingle | Tai-yu CHEN Ji-xiang HOU Yong-jun LIN Transcriptome datasets supply basic gene information for RNAi pest management and gene functional studies in Nephotettix cincticeps (Uhler) Journal of Integrative Agriculture Nephotettix cincticeps transcriptome RNA interference house-keeping genes |
| title | Transcriptome datasets supply basic gene information for RNAi pest management and gene functional studies in Nephotettix cincticeps (Uhler) |
| title_full | Transcriptome datasets supply basic gene information for RNAi pest management and gene functional studies in Nephotettix cincticeps (Uhler) |
| title_fullStr | Transcriptome datasets supply basic gene information for RNAi pest management and gene functional studies in Nephotettix cincticeps (Uhler) |
| title_full_unstemmed | Transcriptome datasets supply basic gene information for RNAi pest management and gene functional studies in Nephotettix cincticeps (Uhler) |
| title_short | Transcriptome datasets supply basic gene information for RNAi pest management and gene functional studies in Nephotettix cincticeps (Uhler) |
| title_sort | transcriptome datasets supply basic gene information for rnai pest management and gene functional studies in nephotettix cincticeps uhler |
| topic | Nephotettix cincticeps transcriptome RNA interference house-keeping genes |
| url | http://www.sciencedirect.com/science/article/pii/S2095311915611339 |
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