Sexual Differentiation and Primordial Germ Cell Distribution in the Early Horse Fetus
It was the aim of this study to characterize the development of the gonads and genital ducts in the equine fetus around the time of sexual differentiation. This included the identification and localization of the primordial germ cell population. Equine fetuses between 45 and 60 days of gestation wer...
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MDPI AG
2021-08-01
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| Online Access: | https://www.mdpi.com/2076-2615/11/8/2422 |
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| author | Dragos Scarlet Stephan Handschuh Ursula Reichart Giorgia Podico Robyn E. Ellerbrock Sebastián Demyda-Peyrás Igor F. Canisso Ingrid Walter Christine Aurich |
| author_facet | Dragos Scarlet Stephan Handschuh Ursula Reichart Giorgia Podico Robyn E. Ellerbrock Sebastián Demyda-Peyrás Igor F. Canisso Ingrid Walter Christine Aurich |
| author_sort | Dragos Scarlet |
| collection | DOAJ |
| description | It was the aim of this study to characterize the development of the gonads and genital ducts in the equine fetus around the time of sexual differentiation. This included the identification and localization of the primordial germ cell population. Equine fetuses between 45 and 60 days of gestation were evaluated using a combination of micro-computed tomography scanning, immunohistochemistry, and multiplex immunofluorescence. Fetal gonads increased in size 23-fold from 45 to 60 days of gestation, and an even greater increase was observed in the metanephros volume. Signs of mesonephros atrophy were detected during this time. Tubular structures of the fetal testes were present from day 50 onwards, whereas cell clusters dominated in the fetal ovary. The genital ducts were well-differentiated and presented a lumen in all samples. No sign of mesonephric or paramesonephric duct degeneration was detected. Expression of AMH was strong in the fetal testes but absent in ovaries. Irrespective of sex, primordial germ cells selectively expressed LIN28. Migration of primordial germ cells from the mesonephros to the gonad was detected at 45 days, but not at 60 days of development. Their number and distribution within the gonad were influenced (<i>p</i> < 0.05) by fetal sex. Most primordial germ cells (86.8 ± 3.2% in females and 84.6 ± 4.7% in males) were characterized as pluripotent according to co-localization with CD117. However, only a very small percentage of primordial germ cells were proliferating (7.5 ± 1.7% in females and 3.2 ± 1.2% in males) based on co-localization with Ki67. It can be concluded that gonadal sexual differentiation in the horse occurs asynchronously with regard to sex but already before 45 days of gestation. |
| first_indexed | 2024-03-10T09:04:45Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T09:04:45Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-d64309056c5f4766806a9500527f5e8a2023-11-22T06:31:53ZengMDPI AGAnimals2076-26152021-08-01118242210.3390/ani11082422Sexual Differentiation and Primordial Germ Cell Distribution in the Early Horse FetusDragos Scarlet0Stephan Handschuh1Ursula Reichart2Giorgia Podico3Robyn E. Ellerbrock4Sebastián Demyda-Peyrás5Igor F. Canisso6Ingrid Walter7Christine Aurich8Obstetrics, Gynecology and Andrology, Department for Small Animals and Horses, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, AustriaVetcore Facility for Research, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, AustriaVetcore Facility for Research, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, AustriaDepartment of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana-Champaign, Urbana, IL 61802, USADepartment of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana-Champaign, Urbana, IL 61802, USADepartment of Animal Production, School of Veterinary Sciences, National University of La Plata and CONICET CCT-La Plata, Calle 60 and 118 S/N, 1900 La Plata, ArgentinaDepartment of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana-Champaign, Urbana, IL 61802, USAVetcore Facility for Research, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, AustriaCenter for Artificial Insemination and Embryo Transfer, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, AustriaIt was the aim of this study to characterize the development of the gonads and genital ducts in the equine fetus around the time of sexual differentiation. This included the identification and localization of the primordial germ cell population. Equine fetuses between 45 and 60 days of gestation were evaluated using a combination of micro-computed tomography scanning, immunohistochemistry, and multiplex immunofluorescence. Fetal gonads increased in size 23-fold from 45 to 60 days of gestation, and an even greater increase was observed in the metanephros volume. Signs of mesonephros atrophy were detected during this time. Tubular structures of the fetal testes were present from day 50 onwards, whereas cell clusters dominated in the fetal ovary. The genital ducts were well-differentiated and presented a lumen in all samples. No sign of mesonephric or paramesonephric duct degeneration was detected. Expression of AMH was strong in the fetal testes but absent in ovaries. Irrespective of sex, primordial germ cells selectively expressed LIN28. Migration of primordial germ cells from the mesonephros to the gonad was detected at 45 days, but not at 60 days of development. Their number and distribution within the gonad were influenced (<i>p</i> < 0.05) by fetal sex. Most primordial germ cells (86.8 ± 3.2% in females and 84.6 ± 4.7% in males) were characterized as pluripotent according to co-localization with CD117. However, only a very small percentage of primordial germ cells were proliferating (7.5 ± 1.7% in females and 3.2 ± 1.2% in males) based on co-localization with Ki67. It can be concluded that gonadal sexual differentiation in the horse occurs asynchronously with regard to sex but already before 45 days of gestation.https://www.mdpi.com/2076-2615/11/8/2422gonadgerm cellgenital ductfetushorse |
| spellingShingle | Dragos Scarlet Stephan Handschuh Ursula Reichart Giorgia Podico Robyn E. Ellerbrock Sebastián Demyda-Peyrás Igor F. Canisso Ingrid Walter Christine Aurich Sexual Differentiation and Primordial Germ Cell Distribution in the Early Horse Fetus Animals gonad germ cell genital duct fetus horse |
| title | Sexual Differentiation and Primordial Germ Cell Distribution in the Early Horse Fetus |
| title_full | Sexual Differentiation and Primordial Germ Cell Distribution in the Early Horse Fetus |
| title_fullStr | Sexual Differentiation and Primordial Germ Cell Distribution in the Early Horse Fetus |
| title_full_unstemmed | Sexual Differentiation and Primordial Germ Cell Distribution in the Early Horse Fetus |
| title_short | Sexual Differentiation and Primordial Germ Cell Distribution in the Early Horse Fetus |
| title_sort | sexual differentiation and primordial germ cell distribution in the early horse fetus |
| topic | gonad germ cell genital duct fetus horse |
| url | https://www.mdpi.com/2076-2615/11/8/2422 |
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