Aminophylline Pretreatment Modulates the Nitric Oxide System of the Isolated Rat Diaphragm: the Role of Frequency of the Electrical Stimulation
The effect of different frequencies of direct subtetanic electrical stimulation (14, 20, and 30 Hz) and aminophylline (AMPh) pretreatment on the effect of NG-nitro-L-arginine methyl ester (L-NAME) (1 mM) on isolated rat diaphragm was investigated. L-NAME potentiated tension developed (Td) in the dia...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2005-01-01
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| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861319321401 |
| _version_ | 1818279562589503488 |
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| author | Radan Stojanovi Zoran Todorović Zorica Nešić Sonja Vučković Milica Prostran |
| author_facet | Radan Stojanovi Zoran Todorović Zorica Nešić Sonja Vučković Milica Prostran |
| author_sort | Radan Stojanovi |
| collection | DOAJ |
| description | The effect of different frequencies of direct subtetanic electrical stimulation (14, 20, and 30 Hz) and aminophylline (AMPh) pretreatment on the effect of NG-nitro-L-arginine methyl ester (L-NAME) (1 mM) on isolated rat diaphragm was investigated. L-NAME potentiated tension developed (Td) in the diaphragm pretreated with a single concentration of AMPh (1.08 mM) in a frequency-dependent manner. The effect was significantly different in comparison with muscle incubated 30 min with Tyrode solution only. In the muscle pretreated with cumulative concentrations of AMPh (0.36 – 3.60 mM), the frequency-dependent potentiation of Td induced by L-NAME and the difference between L-NAME-treated and untreated muscle were lost. Keywords:: aminophylline, NG-nitro-L-arginine methyl ester, stimulation frequency |
| first_indexed | 2024-12-12T23:35:18Z |
| format | Article |
| id | doaj.art-d6432adea8be4ccfab1af8f72070a270 |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-12-12T23:35:18Z |
| publishDate | 2005-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-d6432adea8be4ccfab1af8f72070a2702022-12-22T00:07:31ZengElsevierJournal of Pharmacological Sciences1347-86132005-01-01991109112Aminophylline Pretreatment Modulates the Nitric Oxide System of the Isolated Rat Diaphragm: the Role of Frequency of the Electrical StimulationRadan Stojanovi0Zoran Todorović1Zorica Nešić2Sonja Vučković3Milica Prostran4Department of Pharmacology, Clinical Pharmacology and Toxicology, School of Medicine University of Belgrade, Belgrade, P.O.Box 840, 11129 Belgrade, Serbia and MontenegroDepartment of Pharmacology, Clinical Pharmacology and Toxicology, School of Medicine University of Belgrade, Belgrade, P.O.Box 840, 11129 Belgrade, Serbia and MontenegroDepartment of Pharmacology, Clinical Pharmacology and Toxicology, School of Medicine University of Belgrade, Belgrade, P.O.Box 840, 11129 Belgrade, Serbia and MontenegroDepartment of Pharmacology, Clinical Pharmacology and Toxicology, School of Medicine University of Belgrade, Belgrade, P.O.Box 840, 11129 Belgrade, Serbia and MontenegroDepartment of Pharmacology, Clinical Pharmacology and Toxicology, School of Medicine University of Belgrade, Belgrade, P.O.Box 840, 11129 Belgrade, Serbia and Montenegro; Corresponding author. FAX: +381-11-686-025 E-mail: mprostran@doctor.comThe effect of different frequencies of direct subtetanic electrical stimulation (14, 20, and 30 Hz) and aminophylline (AMPh) pretreatment on the effect of NG-nitro-L-arginine methyl ester (L-NAME) (1 mM) on isolated rat diaphragm was investigated. L-NAME potentiated tension developed (Td) in the diaphragm pretreated with a single concentration of AMPh (1.08 mM) in a frequency-dependent manner. The effect was significantly different in comparison with muscle incubated 30 min with Tyrode solution only. In the muscle pretreated with cumulative concentrations of AMPh (0.36 – 3.60 mM), the frequency-dependent potentiation of Td induced by L-NAME and the difference between L-NAME-treated and untreated muscle were lost. Keywords:: aminophylline, NG-nitro-L-arginine methyl ester, stimulation frequencyhttp://www.sciencedirect.com/science/article/pii/S1347861319321401 |
| spellingShingle | Radan Stojanovi Zoran Todorović Zorica Nešić Sonja Vučković Milica Prostran Aminophylline Pretreatment Modulates the Nitric Oxide System of the Isolated Rat Diaphragm: the Role of Frequency of the Electrical Stimulation Journal of Pharmacological Sciences |
| title | Aminophylline Pretreatment Modulates the Nitric Oxide System of the Isolated Rat Diaphragm: the Role of Frequency of the Electrical Stimulation |
| title_full | Aminophylline Pretreatment Modulates the Nitric Oxide System of the Isolated Rat Diaphragm: the Role of Frequency of the Electrical Stimulation |
| title_fullStr | Aminophylline Pretreatment Modulates the Nitric Oxide System of the Isolated Rat Diaphragm: the Role of Frequency of the Electrical Stimulation |
| title_full_unstemmed | Aminophylline Pretreatment Modulates the Nitric Oxide System of the Isolated Rat Diaphragm: the Role of Frequency of the Electrical Stimulation |
| title_short | Aminophylline Pretreatment Modulates the Nitric Oxide System of the Isolated Rat Diaphragm: the Role of Frequency of the Electrical Stimulation |
| title_sort | aminophylline pretreatment modulates the nitric oxide system of the isolated rat diaphragm the role of frequency of the electrical stimulation |
| url | http://www.sciencedirect.com/science/article/pii/S1347861319321401 |
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