Identification, characterization and expression analysis of wheat RSH family genes under abiotic stress
Guanosine pentaphosphate and guanosine tetraphosphate are collectively called (p)ppGpp (Guanosine tetraphosphate and pentaphosphate). (p)ppGpp content in plants is affected by conditions such as light, salt, pH, UV light, and environmental phytohormones. The synthesis and hydrolysis of (p)ppGpp in p...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1283567/full |
| _version_ | 1827632596499038208 |
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| author | Mengru Wang Wei Hong Youning Wang Xiaowen Han Wang Chen Shuping Wang Yingxin Zhang Wenli Wang |
| author_facet | Mengru Wang Wei Hong Youning Wang Xiaowen Han Wang Chen Shuping Wang Yingxin Zhang Wenli Wang |
| author_sort | Mengru Wang |
| collection | DOAJ |
| description | Guanosine pentaphosphate and guanosine tetraphosphate are collectively called (p)ppGpp (Guanosine tetraphosphate and pentaphosphate). (p)ppGpp content in plants is affected by conditions such as light, salt, pH, UV light, and environmental phytohormones. The synthesis and hydrolysis of (p)ppGpp in plants is accomplished by a class of proteins called RSH (RelA/SpoT homologs). To date, a systematic and comprehensive genome-wide analysis of the RSH gene family in wheat and its closely related species has not been conducted. In this study, 15, 14, 12, and 8 members of RSH were identified in wheat (Triticum aestivum), Triticum dicoccoides, Triticum urartu and Aegilops tauschii respectively. Based on the conserved structural domains of the RSH genes, the TaRSHs have been categorized into TaRSH and TaCRSH. The gene duplications in the TaRSH gene family were all identified as segmental duplications indicating that the TaRSH family plays a significant role in expansion and that segmental duplications maintain a degree of genetic stability. Through the analysis of transcriptome data and RT-qPCR experiments, it was observed that the expression levels of TaRSHs were upregulated in response to abiotic stress. This upregulation suggests that TaRSHs play a crucial role in enhancing the resilience of wheat to adverse environmental conditions during its growth and development. Their increased expression likely contributes to the acquisition of stress tolerance mechanisms in wheat. Especially under NaCl stress, the expression levels increased most significantly. The more detailed systematic analysis provided in this article will help us understand the role of TaRSHs and provide a reference for further research on its molecular biological functions in wheat. |
| first_indexed | 2024-03-09T14:46:52Z |
| format | Article |
| id | doaj.art-d6448cdeaea842ef9bc0188b996cb825 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-09T14:46:52Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-d6448cdeaea842ef9bc0188b996cb8252023-11-27T04:30:16ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-11-011410.3389/fpls.2023.12835671283567Identification, characterization and expression analysis of wheat RSH family genes under abiotic stressMengru Wang0Wei Hong1Youning Wang2Xiaowen Han3Wang Chen4Shuping Wang5Yingxin Zhang6Wenli Wang7Ministry of Agriculture and Rural Affairs (MARA) Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-Construction by Ministry and Province), College of Agriculture, Yangtze University, Jingzhou, ChinaMinistry of Agriculture and Rural Affairs (MARA) Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-Construction by Ministry and Province), College of Agriculture, Yangtze University, Jingzhou, ChinaHubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables, Hubei Engineering University, Xiaogan, Hubei, ChinaMinistry of Agriculture and Rural Affairs (MARA) Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-Construction by Ministry and Province), College of Agriculture, Yangtze University, Jingzhou, ChinaMinistry of Agriculture and Rural Affairs (MARA) Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-Construction by Ministry and Province), College of Agriculture, Yangtze University, Jingzhou, ChinaMinistry of Agriculture and Rural Affairs (MARA) Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-Construction by Ministry and Province), College of Agriculture, Yangtze University, Jingzhou, ChinaMinistry of Agriculture and Rural Affairs (MARA) Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-Construction by Ministry and Province), College of Agriculture, Yangtze University, Jingzhou, ChinaCollege of Plant Protection, North West Agriculture and Forestry (A&F), University, Yangling, Shaanxi, ChinaGuanosine pentaphosphate and guanosine tetraphosphate are collectively called (p)ppGpp (Guanosine tetraphosphate and pentaphosphate). (p)ppGpp content in plants is affected by conditions such as light, salt, pH, UV light, and environmental phytohormones. The synthesis and hydrolysis of (p)ppGpp in plants is accomplished by a class of proteins called RSH (RelA/SpoT homologs). To date, a systematic and comprehensive genome-wide analysis of the RSH gene family in wheat and its closely related species has not been conducted. In this study, 15, 14, 12, and 8 members of RSH were identified in wheat (Triticum aestivum), Triticum dicoccoides, Triticum urartu and Aegilops tauschii respectively. Based on the conserved structural domains of the RSH genes, the TaRSHs have been categorized into TaRSH and TaCRSH. The gene duplications in the TaRSH gene family were all identified as segmental duplications indicating that the TaRSH family plays a significant role in expansion and that segmental duplications maintain a degree of genetic stability. Through the analysis of transcriptome data and RT-qPCR experiments, it was observed that the expression levels of TaRSHs were upregulated in response to abiotic stress. This upregulation suggests that TaRSHs play a crucial role in enhancing the resilience of wheat to adverse environmental conditions during its growth and development. Their increased expression likely contributes to the acquisition of stress tolerance mechanisms in wheat. Especially under NaCl stress, the expression levels increased most significantly. The more detailed systematic analysis provided in this article will help us understand the role of TaRSHs and provide a reference for further research on its molecular biological functions in wheat.https://www.frontiersin.org/articles/10.3389/fpls.2023.1283567/fullRSH genesbioinformaticsRT-qPCRtranscriptome analysisabiotic stress |
| spellingShingle | Mengru Wang Wei Hong Youning Wang Xiaowen Han Wang Chen Shuping Wang Yingxin Zhang Wenli Wang Identification, characterization and expression analysis of wheat RSH family genes under abiotic stress Frontiers in Plant Science RSH genes bioinformatics RT-qPCR transcriptome analysis abiotic stress |
| title | Identification, characterization and expression analysis of wheat RSH family genes under abiotic stress |
| title_full | Identification, characterization and expression analysis of wheat RSH family genes under abiotic stress |
| title_fullStr | Identification, characterization and expression analysis of wheat RSH family genes under abiotic stress |
| title_full_unstemmed | Identification, characterization and expression analysis of wheat RSH family genes under abiotic stress |
| title_short | Identification, characterization and expression analysis of wheat RSH family genes under abiotic stress |
| title_sort | identification characterization and expression analysis of wheat rsh family genes under abiotic stress |
| topic | RSH genes bioinformatics RT-qPCR transcriptome analysis abiotic stress |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1283567/full |
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