Chromosomal Aberrations in <it>ETV6/RUNX1</it>-positive Childhood Acute Lymphoblastic Leukemia using 244K Oligonucleotide Array Comparative Genomic Hybridization
<p>Abstract</p> <p>Background</p> <p>Acute lymphoblastic leukemia (ALL) is a heterogeneous form of hematological cancer consisting of various subtypes. We are interested to study the genetic aberration in precursor B-cell ALL with specific t(12;21) translocation in chil...
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BMC
2012-11-01
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| Series: | Molecular Cytogenetics |
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| Online Access: | http://www.molecularcytogenetics.org/content/5/1/41 |
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| author | Zakaria Zubaidah Ahid Mohd Fadly Md Ismail Azli Keoh Ten Sew Nor Nooraisyah Mohamad Kamaluddin Nor Rizan Esa Ezalia Yuen Lam Kah Rahman Eni Juraida Abdul Osman Raudhawati |
| author_facet | Zakaria Zubaidah Ahid Mohd Fadly Md Ismail Azli Keoh Ten Sew Nor Nooraisyah Mohamad Kamaluddin Nor Rizan Esa Ezalia Yuen Lam Kah Rahman Eni Juraida Abdul Osman Raudhawati |
| author_sort | Zakaria Zubaidah |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Acute lymphoblastic leukemia (ALL) is a heterogeneous form of hematological cancer consisting of various subtypes. We are interested to study the genetic aberration in precursor B-cell ALL with specific t(12;21) translocation in childhood ALL patients. A high resolution 244K array-based Comparative Genomic Hybridization (array-CGH) was used to study eleven <it>ETV6/RUNX1</it>-positive childhood acute lymphoblastic leukemia (ALL) patients.</p> <p>Result</p> <p>155 chromosomal aberrations (119 losses, 36 gains) were reported in the array findings, corresponding to 76.8% deletions and 23.2% amplifications. The <it>ETV6</it> gene deletion occurred in 4 of the patients, corresponding to 45% of the sample. The most common alterations above 1 Mb were deletion 6q (13%), 12p (12%) and 9p (8%), and duplication 4q (6%) and Xq (4%). Other genes important in ALL were also identified in this study including <it>RUNX1, CDKN2A, FHIT</it>, and <it>PAX5</it>. The array-CGH technique was able to detect microdeletion as small as 400 bp.</p> <p>Conclusion</p> <p>The results demonstrate the usefulness of high resolution array-CGH as a complementary tool in the investigation of ALL.</p> |
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| issn | 1755-8166 |
| language | English |
| last_indexed | 2024-04-12T16:43:42Z |
| publishDate | 2012-11-01 |
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| series | Molecular Cytogenetics |
| spelling | doaj.art-d6456f97d2d34060b118eef1cc2a438b2022-12-22T03:24:41ZengBMCMolecular Cytogenetics1755-81662012-11-01514110.1186/1755-8166-5-41Chromosomal Aberrations in <it>ETV6/RUNX1</it>-positive Childhood Acute Lymphoblastic Leukemia using 244K Oligonucleotide Array Comparative Genomic HybridizationZakaria ZubaidahAhid Mohd Fadly MdIsmail AzliKeoh Ten SewNor Nooraisyah MohamadKamaluddin Nor RizanEsa EzaliaYuen Lam KahRahman Eni Juraida AbdulOsman Raudhawati<p>Abstract</p> <p>Background</p> <p>Acute lymphoblastic leukemia (ALL) is a heterogeneous form of hematological cancer consisting of various subtypes. We are interested to study the genetic aberration in precursor B-cell ALL with specific t(12;21) translocation in childhood ALL patients. A high resolution 244K array-based Comparative Genomic Hybridization (array-CGH) was used to study eleven <it>ETV6/RUNX1</it>-positive childhood acute lymphoblastic leukemia (ALL) patients.</p> <p>Result</p> <p>155 chromosomal aberrations (119 losses, 36 gains) were reported in the array findings, corresponding to 76.8% deletions and 23.2% amplifications. The <it>ETV6</it> gene deletion occurred in 4 of the patients, corresponding to 45% of the sample. The most common alterations above 1 Mb were deletion 6q (13%), 12p (12%) and 9p (8%), and duplication 4q (6%) and Xq (4%). Other genes important in ALL were also identified in this study including <it>RUNX1, CDKN2A, FHIT</it>, and <it>PAX5</it>. The array-CGH technique was able to detect microdeletion as small as 400 bp.</p> <p>Conclusion</p> <p>The results demonstrate the usefulness of high resolution array-CGH as a complementary tool in the investigation of ALL.</p>http://www.molecularcytogenetics.org/content/5/1/41Array-based Comparative Genomic HybridizationAcute lymphoblastic leukemia<it>ETV6/RUNX1</it> |
| spellingShingle | Zakaria Zubaidah Ahid Mohd Fadly Md Ismail Azli Keoh Ten Sew Nor Nooraisyah Mohamad Kamaluddin Nor Rizan Esa Ezalia Yuen Lam Kah Rahman Eni Juraida Abdul Osman Raudhawati Chromosomal Aberrations in <it>ETV6/RUNX1</it>-positive Childhood Acute Lymphoblastic Leukemia using 244K Oligonucleotide Array Comparative Genomic Hybridization Molecular Cytogenetics Array-based Comparative Genomic Hybridization Acute lymphoblastic leukemia <it>ETV6/RUNX1</it> |
| title | Chromosomal Aberrations in <it>ETV6/RUNX1</it>-positive Childhood Acute Lymphoblastic Leukemia using 244K Oligonucleotide Array Comparative Genomic Hybridization |
| title_full | Chromosomal Aberrations in <it>ETV6/RUNX1</it>-positive Childhood Acute Lymphoblastic Leukemia using 244K Oligonucleotide Array Comparative Genomic Hybridization |
| title_fullStr | Chromosomal Aberrations in <it>ETV6/RUNX1</it>-positive Childhood Acute Lymphoblastic Leukemia using 244K Oligonucleotide Array Comparative Genomic Hybridization |
| title_full_unstemmed | Chromosomal Aberrations in <it>ETV6/RUNX1</it>-positive Childhood Acute Lymphoblastic Leukemia using 244K Oligonucleotide Array Comparative Genomic Hybridization |
| title_short | Chromosomal Aberrations in <it>ETV6/RUNX1</it>-positive Childhood Acute Lymphoblastic Leukemia using 244K Oligonucleotide Array Comparative Genomic Hybridization |
| title_sort | chromosomal aberrations in it etv6 runx1 it positive childhood acute lymphoblastic leukemia using 244k oligonucleotide array comparative genomic hybridization |
| topic | Array-based Comparative Genomic Hybridization Acute lymphoblastic leukemia <it>ETV6/RUNX1</it> |
| url | http://www.molecularcytogenetics.org/content/5/1/41 |
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