Single-Cell Transcriptomic Analysis of the Mouse Pancreas: Characteristic Features of Pancreatic Ductal Cells in Chronic Pancreatitis
Chronic pancreatitis (CP) is a fibroinflammatory disorder of the pancreas. Our understanding of CP pathogenesis is partly limited by the incomplete characterization of pancreatic cell types. Here, we performed single-cell RNA sequencing on 3825 cells from the pancreas of one control mouse and mice w...
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MDPI AG
2022-06-01
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| Online Access: | https://www.mdpi.com/2073-4425/13/6/1015 |
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| author | Xiaotong Mao Shenghan Mao Lei Wang Hui Jiang Shunjiang Deng Yuanchen Wang Jun Ye Zhaoshen Li Wenbin Zou Zhuan Liao |
| author_facet | Xiaotong Mao Shenghan Mao Lei Wang Hui Jiang Shunjiang Deng Yuanchen Wang Jun Ye Zhaoshen Li Wenbin Zou Zhuan Liao |
| author_sort | Xiaotong Mao |
| collection | DOAJ |
| description | Chronic pancreatitis (CP) is a fibroinflammatory disorder of the pancreas. Our understanding of CP pathogenesis is partly limited by the incomplete characterization of pancreatic cell types. Here, we performed single-cell RNA sequencing on 3825 cells from the pancreas of one control mouse and mice with caerulein-induced CP. An analysis of the single-cell transcriptomes revealed 16 unique clusters and cell type-specific gene expression patterns in the mouse pancreas. Sub-clustering of the pancreatic mesenchymal cells from the control mouse revealed four clusters of cells with specific gene expression profiles (combinatorial expressions of <i>Smoc2</i>, <i>Cxcl14</i>, <i>Tnfaip6</i>, and <i>Fn1</i>). We observed that immune cells in the pancreas of the CP mice were abundant and diverse in cellular type. Compared to the control, 547 upregulated genes (including <i>Mmp7</i>, <i>Ttr</i>, <i>Rgs5</i>, <i>Adh1</i>, and <i>Cldn2</i>) and 257 downregulated genes were identified in ductal cells from the CP group. The elevated expression levels of MMP7 and TTR were further verified in the pancreatic ducts of CP patients. This study provides a preliminary description of the single-cell transcriptome profiles of mouse pancreata and accurately demonstrates the characteristics of pancreatic ductal cells in CP. The findings provide insight into novel disease-specific biomarkers and potential therapeutic targets of CP. |
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| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T23:44:14Z |
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| series | Genes |
| spelling | doaj.art-d646446c2fdb44afbe901405c356f3f42023-11-23T16:47:53ZengMDPI AGGenes2073-44252022-06-01136101510.3390/genes13061015Single-Cell Transcriptomic Analysis of the Mouse Pancreas: Characteristic Features of Pancreatic Ductal Cells in Chronic PancreatitisXiaotong Mao0Shenghan Mao1Lei Wang2Hui Jiang3Shunjiang Deng4Yuanchen Wang5Jun Ye6Zhaoshen Li7Wenbin Zou8Zhuan Liao9Department of Gastroenterology, Changhai Hospital, The Second Military Medical University, Shanghai 200433, ChinaDepartment of Gastroenterology, Changhai Hospital, The Second Military Medical University, Shanghai 200433, ChinaDepartment of Gastroenterology, Changhai Hospital, The Second Military Medical University, Shanghai 200433, ChinaDepartment of Pathology, Changhai Hospital, The Second Military Medical University, Shanghai 200433, ChinaDepartment of Gastroenterology, Changhai Hospital, The Second Military Medical University, Shanghai 200433, ChinaDepartment of Gastroenterology, Changhai Hospital, The Second Military Medical University, Shanghai 200433, ChinaDepartment of Gastroenterology, Changhai Hospital, The Second Military Medical University, Shanghai 200433, ChinaDepartment of Gastroenterology, Changhai Hospital, The Second Military Medical University, Shanghai 200433, ChinaShanghai Institute of Pancreatic Diseases, Shanghai 200433, ChinaDepartment of Gastroenterology, Changhai Hospital, The Second Military Medical University, Shanghai 200433, ChinaChronic pancreatitis (CP) is a fibroinflammatory disorder of the pancreas. Our understanding of CP pathogenesis is partly limited by the incomplete characterization of pancreatic cell types. Here, we performed single-cell RNA sequencing on 3825 cells from the pancreas of one control mouse and mice with caerulein-induced CP. An analysis of the single-cell transcriptomes revealed 16 unique clusters and cell type-specific gene expression patterns in the mouse pancreas. Sub-clustering of the pancreatic mesenchymal cells from the control mouse revealed four clusters of cells with specific gene expression profiles (combinatorial expressions of <i>Smoc2</i>, <i>Cxcl14</i>, <i>Tnfaip6</i>, and <i>Fn1</i>). We observed that immune cells in the pancreas of the CP mice were abundant and diverse in cellular type. Compared to the control, 547 upregulated genes (including <i>Mmp7</i>, <i>Ttr</i>, <i>Rgs5</i>, <i>Adh1</i>, and <i>Cldn2</i>) and 257 downregulated genes were identified in ductal cells from the CP group. The elevated expression levels of MMP7 and TTR were further verified in the pancreatic ducts of CP patients. This study provides a preliminary description of the single-cell transcriptome profiles of mouse pancreata and accurately demonstrates the characteristics of pancreatic ductal cells in CP. The findings provide insight into novel disease-specific biomarkers and potential therapeutic targets of CP.https://www.mdpi.com/2073-4425/13/6/1015chronic pancreatitissingle-cell analysistranscriptomemouse modelcaeruleinmesenchymal cells |
| spellingShingle | Xiaotong Mao Shenghan Mao Lei Wang Hui Jiang Shunjiang Deng Yuanchen Wang Jun Ye Zhaoshen Li Wenbin Zou Zhuan Liao Single-Cell Transcriptomic Analysis of the Mouse Pancreas: Characteristic Features of Pancreatic Ductal Cells in Chronic Pancreatitis Genes chronic pancreatitis single-cell analysis transcriptome mouse model caerulein mesenchymal cells |
| title | Single-Cell Transcriptomic Analysis of the Mouse Pancreas: Characteristic Features of Pancreatic Ductal Cells in Chronic Pancreatitis |
| title_full | Single-Cell Transcriptomic Analysis of the Mouse Pancreas: Characteristic Features of Pancreatic Ductal Cells in Chronic Pancreatitis |
| title_fullStr | Single-Cell Transcriptomic Analysis of the Mouse Pancreas: Characteristic Features of Pancreatic Ductal Cells in Chronic Pancreatitis |
| title_full_unstemmed | Single-Cell Transcriptomic Analysis of the Mouse Pancreas: Characteristic Features of Pancreatic Ductal Cells in Chronic Pancreatitis |
| title_short | Single-Cell Transcriptomic Analysis of the Mouse Pancreas: Characteristic Features of Pancreatic Ductal Cells in Chronic Pancreatitis |
| title_sort | single cell transcriptomic analysis of the mouse pancreas characteristic features of pancreatic ductal cells in chronic pancreatitis |
| topic | chronic pancreatitis single-cell analysis transcriptome mouse model caerulein mesenchymal cells |
| url | https://www.mdpi.com/2073-4425/13/6/1015 |
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