Core Microbiome and Microbial Community Structure in Coralloid Roots of <i>Cycas</i> in Ex Situ Collection of Kunming Botanical Garden in China
Endophytes are essential in plant succession and evolution, and essential for stress resistance. Coralloid root is a unique root structure found in cycads that has played a role in resisting adverse environments, yet the core taxa and microbial community of different <i>Cycas</i> species...
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MDPI AG
2023-08-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/9/2144 |
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| author | Zhaochun Wang Jian Liu Haiyan Xu Jiating Liu Zhiwei Zhao Xun Gong |
| author_facet | Zhaochun Wang Jian Liu Haiyan Xu Jiating Liu Zhiwei Zhao Xun Gong |
| author_sort | Zhaochun Wang |
| collection | DOAJ |
| description | Endophytes are essential in plant succession and evolution, and essential for stress resistance. Coralloid root is a unique root structure found in cycads that has played a role in resisting adverse environments, yet the core taxa and microbial community of different <i>Cycas</i> species have not been thoroughly investigated. Using amplicon sequencing, we successfully elucidated the microbiomes present in coralloid roots of 10 <i>Cycas</i> species, representing all four sections of <i>Cycas</i> in China. We found that the endophytic bacteria in coralloid roots, i.e., <i>Cyanobacteria</i>, were mainly composed of <i>Desmonostoc</i>_PCC-7422, <i>Nostoc</i>_PCC-73102 and <i>unclassified_f__Nostocaceae</i>. Additionally, the Ascomycota fungi of <i>Exophiala</i>, <i>Paraboeremia</i>, <i>Leptobacillium</i>, <i>Fusarium</i>, <i>Alternaria</i>, and <i>Diaporthe</i> were identified as the core fungi taxa. The Ascomycota fungi of Nectriaceae, Herpotrichiellaceae, Cordycipitaceae, Helotiaceae, Diaporthaceae, Didymellaceae, Clavicipitaceae and Pleosporaceae were identified as the core family taxa in coralloid roots of four sections. High abundance but low diversity of bacterial community was detected in the coralloid roots, but no significant difference among species. The fungal community exhibited much higher complexity compared to bacteria, and diversity was noted among different species or sections. These core taxa, which were a subset of the microbiome that frequently occurred in all, or most, individuals of <i>Cycas</i> species, represent targets for the development of <i>Cycas</i> conservation. |
| first_indexed | 2024-03-10T22:27:28Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T22:27:28Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-d64b21a934354f44ac5f435e1724a6922023-11-19T12:01:11ZengMDPI AGMicroorganisms2076-26072023-08-01119214410.3390/microorganisms11092144Core Microbiome and Microbial Community Structure in Coralloid Roots of <i>Cycas</i> in Ex Situ Collection of Kunming Botanical Garden in ChinaZhaochun Wang0Jian Liu1Haiyan Xu2Jiating Liu3Zhiwei Zhao4Xun Gong5State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming 650091, ChinaKey Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, ChinaState Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing 100193, ChinaKey Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming 650091, ChinaKey Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, ChinaEndophytes are essential in plant succession and evolution, and essential for stress resistance. Coralloid root is a unique root structure found in cycads that has played a role in resisting adverse environments, yet the core taxa and microbial community of different <i>Cycas</i> species have not been thoroughly investigated. Using amplicon sequencing, we successfully elucidated the microbiomes present in coralloid roots of 10 <i>Cycas</i> species, representing all four sections of <i>Cycas</i> in China. We found that the endophytic bacteria in coralloid roots, i.e., <i>Cyanobacteria</i>, were mainly composed of <i>Desmonostoc</i>_PCC-7422, <i>Nostoc</i>_PCC-73102 and <i>unclassified_f__Nostocaceae</i>. Additionally, the Ascomycota fungi of <i>Exophiala</i>, <i>Paraboeremia</i>, <i>Leptobacillium</i>, <i>Fusarium</i>, <i>Alternaria</i>, and <i>Diaporthe</i> were identified as the core fungi taxa. The Ascomycota fungi of Nectriaceae, Herpotrichiellaceae, Cordycipitaceae, Helotiaceae, Diaporthaceae, Didymellaceae, Clavicipitaceae and Pleosporaceae were identified as the core family taxa in coralloid roots of four sections. High abundance but low diversity of bacterial community was detected in the coralloid roots, but no significant difference among species. The fungal community exhibited much higher complexity compared to bacteria, and diversity was noted among different species or sections. These core taxa, which were a subset of the microbiome that frequently occurred in all, or most, individuals of <i>Cycas</i> species, represent targets for the development of <i>Cycas</i> conservation.https://www.mdpi.com/2076-2607/11/9/2144<i>Cycas</i>coralloid rootcore microbiome<i>Desmonostoc</i><i>Exophiala</i> |
| spellingShingle | Zhaochun Wang Jian Liu Haiyan Xu Jiating Liu Zhiwei Zhao Xun Gong Core Microbiome and Microbial Community Structure in Coralloid Roots of <i>Cycas</i> in Ex Situ Collection of Kunming Botanical Garden in China Microorganisms <i>Cycas</i> coralloid root core microbiome <i>Desmonostoc</i> <i>Exophiala</i> |
| title | Core Microbiome and Microbial Community Structure in Coralloid Roots of <i>Cycas</i> in Ex Situ Collection of Kunming Botanical Garden in China |
| title_full | Core Microbiome and Microbial Community Structure in Coralloid Roots of <i>Cycas</i> in Ex Situ Collection of Kunming Botanical Garden in China |
| title_fullStr | Core Microbiome and Microbial Community Structure in Coralloid Roots of <i>Cycas</i> in Ex Situ Collection of Kunming Botanical Garden in China |
| title_full_unstemmed | Core Microbiome and Microbial Community Structure in Coralloid Roots of <i>Cycas</i> in Ex Situ Collection of Kunming Botanical Garden in China |
| title_short | Core Microbiome and Microbial Community Structure in Coralloid Roots of <i>Cycas</i> in Ex Situ Collection of Kunming Botanical Garden in China |
| title_sort | core microbiome and microbial community structure in coralloid roots of i cycas i in ex situ collection of kunming botanical garden in china |
| topic | <i>Cycas</i> coralloid root core microbiome <i>Desmonostoc</i> <i>Exophiala</i> |
| url | https://www.mdpi.com/2076-2607/11/9/2144 |
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