Using Bacteriophages to Treat Resilient Bacteria Found in Produced Water
Numerous treatment modalities have been employed over the years to eradicate bacterial communities in industrial wastewater. Oxidizing agents and chemical additives, such as ozone, permanganate, glutaraldehyde, and chlorine, are effective in treating microbial contaminants that are typically found i...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | Water |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4441/16/6/797 |
| _version_ | 1797239058169069568 |
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| author | Ramon Sanchez-Rosario Jesus Garcia Vivian Rodriguez Kevin A. Schug Zacariah L. Hildenbrand Ricardo A. Bernal |
| author_facet | Ramon Sanchez-Rosario Jesus Garcia Vivian Rodriguez Kevin A. Schug Zacariah L. Hildenbrand Ricardo A. Bernal |
| author_sort | Ramon Sanchez-Rosario |
| collection | DOAJ |
| description | Numerous treatment modalities have been employed over the years to eradicate bacterial communities in industrial wastewater. Oxidizing agents and chemical additives, such as ozone, permanganate, glutaraldehyde, and chlorine, are effective in treating microbial contaminants that are typically found in domestic wastewater. However, the chemical complexity of water produced from fracking requires novel approaches, because the microbes have developed mechanisms to overcome typical disinfectants. In this work, we test the effectiveness of bacteriophages for the eradication of two model bacteria from produced water: <i>Pseudomonas aeruginosa</i> and <i>Bacillus megaterium</i>. These bacteria were grown in low salinity produced water and exposed to their corresponding phage. Overall, the total inactivation of the <i>P. aeruginosa</i> population was achieved, as well as the inactivation of <i>B. megaterium</i>. These promising results provide a potentially useful tool for bacterial elimination in overall PW treatment, at an industrial scale. Particularly, since phage treatment is a rapid and cost-effective alternative. Moreover, these results fall within the objectives proposed as part of the sustainable development goals adopted worldwide. |
| first_indexed | 2024-04-24T17:45:30Z |
| format | Article |
| id | doaj.art-d64c8374db23401da23105920cbcc42f |
| institution | Directory Open Access Journal |
| issn | 2073-4441 |
| language | English |
| last_indexed | 2024-04-24T17:45:30Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Water |
| spelling | doaj.art-d64c8374db23401da23105920cbcc42f2024-03-27T14:08:09ZengMDPI AGWater2073-44412024-03-0116679710.3390/w16060797Using Bacteriophages to Treat Resilient Bacteria Found in Produced WaterRamon Sanchez-Rosario0Jesus Garcia1Vivian Rodriguez2Kevin A. Schug3Zacariah L. Hildenbrand4Ricardo A. Bernal5Department of Chemistry and Biochemistry, The University of Texas at El Paso, 500 W. University Ave., El Paso, TX 79968, USADepartment of Chemistry and Biochemistry, The University of Texas at El Paso, 500 W. University Ave., El Paso, TX 79968, USADepartment of Chemistry and Biochemistry, The University of Texas at Arlington, 700 Planetarium Place, Arlington, TX 76019, USADepartment of Chemistry and Biochemistry, The University of Texas at Arlington, 700 Planetarium Place, Arlington, TX 76019, USADepartment of Chemistry and Biochemistry, The University of Texas at El Paso, 500 W. University Ave., El Paso, TX 79968, USADepartment of Chemistry and Biochemistry, The University of Texas at El Paso, 500 W. University Ave., El Paso, TX 79968, USANumerous treatment modalities have been employed over the years to eradicate bacterial communities in industrial wastewater. Oxidizing agents and chemical additives, such as ozone, permanganate, glutaraldehyde, and chlorine, are effective in treating microbial contaminants that are typically found in domestic wastewater. However, the chemical complexity of water produced from fracking requires novel approaches, because the microbes have developed mechanisms to overcome typical disinfectants. In this work, we test the effectiveness of bacteriophages for the eradication of two model bacteria from produced water: <i>Pseudomonas aeruginosa</i> and <i>Bacillus megaterium</i>. These bacteria were grown in low salinity produced water and exposed to their corresponding phage. Overall, the total inactivation of the <i>P. aeruginosa</i> population was achieved, as well as the inactivation of <i>B. megaterium</i>. These promising results provide a potentially useful tool for bacterial elimination in overall PW treatment, at an industrial scale. Particularly, since phage treatment is a rapid and cost-effective alternative. Moreover, these results fall within the objectives proposed as part of the sustainable development goals adopted worldwide.https://www.mdpi.com/2073-4441/16/6/797oil and gas productionproduced waterwastewater treatmentbacteriophageshydraulic fracturingbiological treatment |
| spellingShingle | Ramon Sanchez-Rosario Jesus Garcia Vivian Rodriguez Kevin A. Schug Zacariah L. Hildenbrand Ricardo A. Bernal Using Bacteriophages to Treat Resilient Bacteria Found in Produced Water Water oil and gas production produced water wastewater treatment bacteriophages hydraulic fracturing biological treatment |
| title | Using Bacteriophages to Treat Resilient Bacteria Found in Produced Water |
| title_full | Using Bacteriophages to Treat Resilient Bacteria Found in Produced Water |
| title_fullStr | Using Bacteriophages to Treat Resilient Bacteria Found in Produced Water |
| title_full_unstemmed | Using Bacteriophages to Treat Resilient Bacteria Found in Produced Water |
| title_short | Using Bacteriophages to Treat Resilient Bacteria Found in Produced Water |
| title_sort | using bacteriophages to treat resilient bacteria found in produced water |
| topic | oil and gas production produced water wastewater treatment bacteriophages hydraulic fracturing biological treatment |
| url | https://www.mdpi.com/2073-4441/16/6/797 |
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