Analysis of <i>clasp2</i> Transcription Pattern in Male Germ Cells during Spermatogenesis: A Comparative Study in Zebrafish (<i>Danio rerio)</i> and Guppy (<i>Poecilia reticulata</i>)
Cytoplasmic linker-associated protein-2 (CLASP2) is a member of the CLIP-associating proteins (CLASPs) family involved in the structure and function of microtubules and Golgi apparatus. Several studies performed using different mammalian and non-mammalian model organisms reported that CLASP2 control...
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MDPI AG
2023-11-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/23/3617 |
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| author | Serena Ricci Maurizio Lazzari Maria Gabriella Maurizii Valeria Franceschini Liliana Milani Pietro Cacialli |
| author_facet | Serena Ricci Maurizio Lazzari Maria Gabriella Maurizii Valeria Franceschini Liliana Milani Pietro Cacialli |
| author_sort | Serena Ricci |
| collection | DOAJ |
| description | Cytoplasmic linker-associated protein-2 (CLASP2) is a member of the CLIP-associating proteins (CLASPs) family involved in the structure and function of microtubules and Golgi apparatus. Several studies performed using different mammalian and non-mammalian model organisms reported that CLASP2 controls microtubule dynamics and the organization of microtubule networks. In <i>Drosophila</i> and mice, an important role of CLASP2 during the development of germ cell lines has been uncovered. However, no study has clearly defined its role during fish germ cell differentiation. In the present study, we used two excellent aquatic animal models among teleost fish: zebrafish (<i>Danio rerio</i>) and guppy (<i>Poecilia reticulata</i>). Using qPCR, we found that the <i>clasp2</i> transcript level is significantly high in the testis of both fish. Then, by in situ hybridization, we localized the <i>clasp2</i> transcript in the spermatozoa of zebrafish and the spermatozeugmata of guppy. Our data suggest a potential role for this gene in the last stage of spermiogenesis in fish. |
| first_indexed | 2024-03-09T01:56:36Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T01:56:36Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-d64e95bd95264965b0533024b885269f2023-12-08T15:10:28ZengMDPI AGAnimals2076-26152023-11-011323361710.3390/ani13233617Analysis of <i>clasp2</i> Transcription Pattern in Male Germ Cells during Spermatogenesis: A Comparative Study in Zebrafish (<i>Danio rerio)</i> and Guppy (<i>Poecilia reticulata</i>)Serena Ricci0Maurizio Lazzari1Maria Gabriella Maurizii2Valeria Franceschini3Liliana Milani4Pietro Cacialli5Department of Biology, Geology and Environmental Sciences, University of Bologna, 40126 Bologna, ItalyDepartment of Biology, Geology and Environmental Sciences, University of Bologna, 40126 Bologna, ItalyDepartment of Biology, Geology and Environmental Sciences, University of Bologna, 40126 Bologna, ItalyDepartment of Biology, Geology and Environmental Sciences, University of Bologna, 40126 Bologna, ItalyDepartment of Biology, Geology and Environmental Sciences, University of Bologna, 40126 Bologna, ItalyDepartment of Biology, Geology and Environmental Sciences, University of Bologna, 40126 Bologna, ItalyCytoplasmic linker-associated protein-2 (CLASP2) is a member of the CLIP-associating proteins (CLASPs) family involved in the structure and function of microtubules and Golgi apparatus. Several studies performed using different mammalian and non-mammalian model organisms reported that CLASP2 controls microtubule dynamics and the organization of microtubule networks. In <i>Drosophila</i> and mice, an important role of CLASP2 during the development of germ cell lines has been uncovered. However, no study has clearly defined its role during fish germ cell differentiation. In the present study, we used two excellent aquatic animal models among teleost fish: zebrafish (<i>Danio rerio</i>) and guppy (<i>Poecilia reticulata</i>). Using qPCR, we found that the <i>clasp2</i> transcript level is significantly high in the testis of both fish. Then, by in situ hybridization, we localized the <i>clasp2</i> transcript in the spermatozoa of zebrafish and the spermatozeugmata of guppy. Our data suggest a potential role for this gene in the last stage of spermiogenesis in fish.https://www.mdpi.com/2076-2615/13/23/3617CLASPmicrotubulesspermatogenesiszebrafishguppy |
| spellingShingle | Serena Ricci Maurizio Lazzari Maria Gabriella Maurizii Valeria Franceschini Liliana Milani Pietro Cacialli Analysis of <i>clasp2</i> Transcription Pattern in Male Germ Cells during Spermatogenesis: A Comparative Study in Zebrafish (<i>Danio rerio)</i> and Guppy (<i>Poecilia reticulata</i>) Animals CLASP microtubules spermatogenesis zebrafish guppy |
| title | Analysis of <i>clasp2</i> Transcription Pattern in Male Germ Cells during Spermatogenesis: A Comparative Study in Zebrafish (<i>Danio rerio)</i> and Guppy (<i>Poecilia reticulata</i>) |
| title_full | Analysis of <i>clasp2</i> Transcription Pattern in Male Germ Cells during Spermatogenesis: A Comparative Study in Zebrafish (<i>Danio rerio)</i> and Guppy (<i>Poecilia reticulata</i>) |
| title_fullStr | Analysis of <i>clasp2</i> Transcription Pattern in Male Germ Cells during Spermatogenesis: A Comparative Study in Zebrafish (<i>Danio rerio)</i> and Guppy (<i>Poecilia reticulata</i>) |
| title_full_unstemmed | Analysis of <i>clasp2</i> Transcription Pattern in Male Germ Cells during Spermatogenesis: A Comparative Study in Zebrafish (<i>Danio rerio)</i> and Guppy (<i>Poecilia reticulata</i>) |
| title_short | Analysis of <i>clasp2</i> Transcription Pattern in Male Germ Cells during Spermatogenesis: A Comparative Study in Zebrafish (<i>Danio rerio)</i> and Guppy (<i>Poecilia reticulata</i>) |
| title_sort | analysis of i clasp2 i transcription pattern in male germ cells during spermatogenesis a comparative study in zebrafish i danio rerio i and guppy i poecilia reticulata i |
| topic | CLASP microtubules spermatogenesis zebrafish guppy |
| url | https://www.mdpi.com/2076-2615/13/23/3617 |
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