An Emulsion System Based on a Chip Polymerase Chain Reaction
In this paper we describe a novel method for detecting many DNA fragments through efficient amplification by using an emulsion system based on “on-chip†PCR instead of conventional multiplex polymerase chain reaction (PCR). During the preparation of on-chip PCR, a set of primers were immobi...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2008-12-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/13/12/3057/ |
| _version_ | 1811254387147800576 |
|---|---|
| author | Pinfei Yu Qinyu Ge Yunfei Bai Zuhong Lu |
| author_facet | Pinfei Yu Qinyu Ge Yunfei Bai Zuhong Lu |
| author_sort | Pinfei Yu |
| collection | DOAJ |
| description | In this paper we describe a novel method for detecting many DNA fragments through efficient amplification by using an emulsion system based on “on-chip†PCR instead of conventional multiplex polymerase chain reaction (PCR). During the preparation of on-chip PCR, a set of primers were immobilized on a slide and other sets were in an emulsion system. Different emulsion phase primers and other related PCR components were dispersed in different droplets of the emulsion system, and then, due to the thermal instability of emulsion droplets, they would be released onto the surface of the slide after preheating in the first PCR step. To test the above method, we used plasma DNAs from pregnant women who was carrying a male fetus for gender identification. Four different Y chromosome DNA fragments were selected. Results showed that different DNA fragments could be simultaneously amplified with satisfactory results. It is suggested that a simple, convenient and inexpensive on-chip PCR method has been developed. |
| first_indexed | 2024-04-12T17:06:41Z |
| format | Article |
| id | doaj.art-d650aa302d45446ea9a670d6380bfc2b |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-12T17:06:41Z |
| publishDate | 2008-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-d650aa302d45446ea9a670d6380bfc2b2022-12-22T03:23:55ZengMDPI AGMolecules1420-30492008-12-0113123057306810.3390/molecules13123057An Emulsion System Based on a Chip Polymerase Chain ReactionPinfei YuQinyu GeYunfei BaiZuhong LuIn this paper we describe a novel method for detecting many DNA fragments through efficient amplification by using an emulsion system based on “on-chip†PCR instead of conventional multiplex polymerase chain reaction (PCR). During the preparation of on-chip PCR, a set of primers were immobilized on a slide and other sets were in an emulsion system. Different emulsion phase primers and other related PCR components were dispersed in different droplets of the emulsion system, and then, due to the thermal instability of emulsion droplets, they would be released onto the surface of the slide after preheating in the first PCR step. To test the above method, we used plasma DNAs from pregnant women who was carrying a male fetus for gender identification. Four different Y chromosome DNA fragments were selected. Results showed that different DNA fragments could be simultaneously amplified with satisfactory results. It is suggested that a simple, convenient and inexpensive on-chip PCR method has been developed.http://www.mdpi.com/1420-3049/13/12/3057/PCR amplificationEmulsionImmobilized primers |
| spellingShingle | Pinfei Yu Qinyu Ge Yunfei Bai Zuhong Lu An Emulsion System Based on a Chip Polymerase Chain Reaction Molecules PCR amplification Emulsion Immobilized primers |
| title | An Emulsion System Based on a Chip Polymerase Chain Reaction |
| title_full | An Emulsion System Based on a Chip Polymerase Chain Reaction |
| title_fullStr | An Emulsion System Based on a Chip Polymerase Chain Reaction |
| title_full_unstemmed | An Emulsion System Based on a Chip Polymerase Chain Reaction |
| title_short | An Emulsion System Based on a Chip Polymerase Chain Reaction |
| title_sort | emulsion system based on a chip polymerase chain reaction |
| topic | PCR amplification Emulsion Immobilized primers |
| url | http://www.mdpi.com/1420-3049/13/12/3057/ |
| work_keys_str_mv | AT pinfeiyu anemulsionsystembasedonachippolymerasechainreaction AT qinyuge anemulsionsystembasedonachippolymerasechainreaction AT yunfeibai anemulsionsystembasedonachippolymerasechainreaction AT zuhonglu anemulsionsystembasedonachippolymerasechainreaction AT pinfeiyu emulsionsystembasedonachippolymerasechainreaction AT qinyuge emulsionsystembasedonachippolymerasechainreaction AT yunfeibai emulsionsystembasedonachippolymerasechainreaction AT zuhonglu emulsionsystembasedonachippolymerasechainreaction |